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Virtual Reality Augmented Hands-on Cryo-EM Training

虚拟现实增强冷冻电镜实践培训

基本信息

批准号：
10633101
负责人：
Yingjie Chen
金额：
$ 12.25万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10633101
关键词：
3-Dimensional Address Adopted Area Biological Biomedical Research Biophysics COVID-19 pandemic Categories Cell physiology Chemistry Clip Collaborations Color Communities Companions Complement Complex Computer Graphics Computer software Cryo-electron tomography Cryoelectron Microscopy Data Collection Development Plans Devices Disease Outbreaks Education Educational Curriculum Electron Microscope Electron Microscopy Electron Microscopy Facility Environment Equipment Evaluation Event Exercise Feedback Freezing GP2 gene General Population Gestures Goals Hand Health Human Impairment In Situ International Interview Knowledge Learning Macromolecular Complexes Methods Modeling Modernization Nobel Prize Online Systems Outcome Preparation Procedures Process Protocols documentation Research Research Institute Research Personnel Resolution Risk SARS-CoV-2 spike protein Safety Sampling Science Scientist Services Site Specimen Speed Structure Study Subject Supervision Surveys Sustainable Development System Technology Thinness Time Tissue Sample Training Training Activity Universities Vendor Virtual and Augmented reality Virus Virus Diseases Writing Zika Virus cost design experience eye hand coordination fluorescence microscope hazard human subject improved in vivo innovation insight instrument instrumentation learning materials lectures light microscopy multimodality operation outreach particle physical process receptor reconstruction simulation structural biology theories three dimensional structure tool virtual virtual reality web site

项目摘要

Project Summary Cryo-electron microscopy (cryoEM) revolutionized structural biology, providing a powerful method to study biological specimens in their native environment. However, a major bottleneck hindering the wider use of cryoEM is the lack of trained professionals capable of proficiently operating the instruments. CryoEM is complex and involves, in addition to an extensive biophysical theoretical background, operation of multiple devices that requires strict procedures, as well as accurate hand-eye coordination as mistakes in operation can damage expensive instrumentation. Current best-practices for user training involve repeated on-site hands-on practice and training with one-on-one supervision by experts. The high demand for instrument usage and staff assistance for cryoEM data collection often limits the opportunities for hands-on training needed by new cryoEM users. The goal of this project is to develop a virtual reality (VR) augmented interactive training system, CryoVR, to provide virtual hands-on, engaging, and self-paced cryoEM training to familiarize novice users with operational procedures, thereby reducing the need for one-on-one training and on-site practice on costly, in-demand cryoEM devices. Additionally, we see the benefit of this training platform as a review exercise for users who may have gone through long periods between instrument usage. We pursue this goal with three aims: Aim 1) Expanding the current CryoVR project to cover a wider range of instruments in single particle cryoEM, cryo-electron tomography, and cryo-correlative light and electron microscopy. This will address the need for orienting new users to cryoEM instruments, developing virtual mastery over a sequence of operations to perform specific key tasks from plunge-freezing of sample grids to TEM operation, and providing sufficient review for returning non- expert users. It is designed to be an integrated part of the cryoEM education curriculum as a pre-training before onsite hands-on training on the physical instruments; Aim 2) Improving users’ learning experience through new interactive features, such as guided, multimodal learning embedded in VR, enabling lab layout customization, personalized customization to enhance inclusion and accessibility, hazard simulation for safety training, and refining hand-gestures and reinforcing constraints for more faithful simulation of the physical actions; and Aim 3) Dissemination, evaluation, and sustainable development. We will evaluate CryoVR’s instructional materials, their impact, and outcomes through voluntary human subject studies and expert interviews. We will establish distribution channels and sustainable development plans to maximize the long-term impact of CryoVR on the community and public. The proposed approach is innovative in cryoEM training and will be effective, efficient, and engaging due to the applicability of modern VR technologies and education theories. This project is significant as it will greatly reduce the time, cost, and risk of cryoEM training, which in turn will lead to increased capacity for facilities to train new users. The low-cost VR hardware and easily accessible CryoVR software will benefit all cryoEM facilities and their users on a national and international scale.

项目摘要冷冻电子显微镜（cryoEM）彻底改变了结构生物学，提供了一个强大的方法来研究自然环境中的生物标本。然而，阻碍冷冻EM更广泛使用的主要瓶颈缺乏能够熟练操作仪器的训练有素的专业人员。CryoEM很复杂，除了广泛的生物物理学理论背景之外，还涉及多个装置的操作，需要严格的程序，以及准确的手眼协调，因为操作错误可能会损坏昂贵的仪器。目前用户培训的最佳做法包括重复的现场动手实践以及专家一对一监督的培训。对仪器使用和工作人员协助的高需求 cryoEM数据收集的局限性通常限制了新cryoEM用户所需的动手培训机会。的该项目的目标是开发一个虚拟现实（VR）增强交互式培训系统CryoVR，以提供虚拟动手，参与和自定进度的cryoEM培训，以使新手用户熟悉操作程序，从而减少了对一对一培训和现场实践的需要，装置.此外，我们认为这个培训平台的好处是为可能有以下问题的用户提供一个复习练习：在仪器使用之间经历了很长的时间。我们通过三个目标来实现这一目标：目标1）扩大目前的CryoVR项目涵盖了单粒子cryoEM、cryo-electron 断层摄影术和冷冻相关光学和电子显微镜。这将解决新的定向需求，用户cryoEM仪器，发展虚拟掌握一系列操作，以执行特定的关键从样品网格的急冻到TEM操作，并为返回的非专家用户。它被设计为cryoEM教育课程的一个组成部分，作为之前的预培训物理仪器的现场实践培训;目标2）通过新的交互式功能，如嵌入VR的引导式多模式学习，实现实验室布局定制，个性化定制以增强包容性和可访问性，安全培训的危险模拟，以及改进手势和加强约束，以更忠实地模拟身体动作;以及目标3）传播、评价和可持续发展。我们将评估CryoVR的教学材料，通过自愿的人类受试者研究和专家访谈，评估影响和成果。我们将建立分销渠道和可持续发展计划，以最大限度地发挥CryoVR对社区和公众。所提出的方法在冷冻EM培训中具有创新性，并且将是有效、高效的，由于现代VR技术和教育理论的适用性，这个项目是重要的是，它将大大减少冷冻EM培训的时间，成本和风险，这反过来将导致增加培训新用户的设施能力。低成本的VR硬件和易于访问的CryoVR软件将在国家和国际范围内使所有cryoEM设施及其用户受益。