权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Virtual Reality Augmented Hands-on Cryo-EM Training

虚拟现实增强冷冻电镜实践培训

基本信息

批准号：
10633101
负责人：
Yingjie Chen
金额：
$ 12.25万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10633101
关键词：
3-Dimensional Address Adopted Area Biological Biomedical Research Biophysics COVID-19 pandemic Categories Cell physiology Chemistry Clip Collaborations Color Communities Companions Complement Complex Computer Graphics Computer software Cryo-electron tomography Cryoelectron Microscopy Data Collection Development Plans Devices Disease Outbreaks Education Educational Curriculum Electron Microscope Electron Microscopy Electron Microscopy Facility Environment Equipment Evaluation Event Exercise Feedback Freezing GP2 gene General Population Gestures Goals Hand Health Human Impairment In Situ International Interview Knowledge Learning Macromolecular Complexes Methods Modeling Modernization Nobel Prize Online Systems Outcome Preparation Procedures Process Protocols documentation Research Research Institute Research Personnel Resolution Risk SARS-CoV-2 spike protein Safety Sampling Science Scientist Services Site Specimen Speed Structure Study Subject Supervision Surveys Sustainable Development System Technology Thinness Time Tissue Sample Training Training Activity Universities Vendor Virtual and Augmented reality Virus Virus Diseases Writing Zika Virus cost design experience eye hand coordination fluorescence microscope hazard human subject improved in vivo innovation insight instrument instrumentation learning materials lectures light microscopy multimodality operation outreach particle physical process receptor reconstruction simulation structural biology theories three dimensional structure tool virtual virtual reality web site

项目摘要

Project Summary Cryo-electron microscopy (cryoEM) revolutionized structural biology, providing a powerful method to study biological specimens in their native environment. However, a major bottleneck hindering the wider use of cryoEM is the lack of trained professionals capable of proficiently operating the instruments. CryoEM is complex and involves, in addition to an extensive biophysical theoretical background, operation of multiple devices that requires strict procedures, as well as accurate hand-eye coordination as mistakes in operation can damage expensive instrumentation. Current best-practices for user training involve repeated on-site hands-on practice and training with one-on-one supervision by experts. The high demand for instrument usage and staff assistance for cryoEM data collection often limits the opportunities for hands-on training needed by new cryoEM users. The goal of this project is to develop a virtual reality (VR) augmented interactive training system, CryoVR, to provide virtual hands-on, engaging, and self-paced cryoEM training to familiarize novice users with operational procedures, thereby reducing the need for one-on-one training and on-site practice on costly, in-demand cryoEM devices. Additionally, we see the benefit of this training platform as a review exercise for users who may have gone through long periods between instrument usage. We pursue this goal with three aims: Aim 1) Expanding the current CryoVR project to cover a wider range of instruments in single particle cryoEM, cryo-electron tomography, and cryo-correlative light and electron microscopy. This will address the need for orienting new users to cryoEM instruments, developing virtual mastery over a sequence of operations to perform specific key tasks from plunge-freezing of sample grids to TEM operation, and providing sufficient review for returning non- expert users. It is designed to be an integrated part of the cryoEM education curriculum as a pre-training before onsite hands-on training on the physical instruments; Aim 2) Improving users’ learning experience through new interactive features, such as guided, multimodal learning embedded in VR, enabling lab layout customization, personalized customization to enhance inclusion and accessibility, hazard simulation for safety training, and refining hand-gestures and reinforcing constraints for more faithful simulation of the physical actions; and Aim 3) Dissemination, evaluation, and sustainable development. We will evaluate CryoVR’s instructional materials, their impact, and outcomes through voluntary human subject studies and expert interviews. We will establish distribution channels and sustainable development plans to maximize the long-term impact of CryoVR on the community and public. The proposed approach is innovative in cryoEM training and will be effective, efficient, and engaging due to the applicability of modern VR technologies and education theories. This project is significant as it will greatly reduce the time, cost, and risk of cryoEM training, which in turn will lead to increased capacity for facilities to train new users. The low-cost VR hardware and easily accessible CryoVR software will benefit all cryoEM facilities and their users on a national and international scale.

项目总结