Environmental Chemical Body Burden and Prospective Breast Cancer Risk in the Cancer Prevention Study-3 Cohort

癌症预防研究 3 队列中的环境化学物质负担和潜在乳腺癌风险

• 批准号: 10669253
• 负责人: Susan L. Neuhausen
• 金额: $ 65.67万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-20 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669253
• 关键词: 3-Dimensional; Acceleration; Address; Age Years; Aging; American Cancer Society; Animals; Biological; Blood; Blood Banks; Blood specimen; Body Burden; Breast; Breast Cancer Risk Factor; Breast Cancer cell line; Breast Epithelial Cells; Cancer Control; Cancer Etiology; Carcinogenicity Tests; Case/Control Studies; Cells; Chemical Exposure; Chemicals; Cytokeratin; DNA Methylation; Data; Development; Diagnosis; Endocrine; Endocrine Disruptors; Enrollment; Environmental Exposure; Environmental Pollutants; Epithelial Cells; Epithelium; Etiology; Exposure to; Female; Foundations; Future; Genetic Transcription; Genome; Genomics; Goals; Health; Hormones; Human; Link; Malignant Neoplasms; Mammary Neoplasms; Mammary gland; Measurement; Measures; Methodology; Methylation; Molecular; Mus; Myoepithelial cell; Outcome; Participant; Perimenopause; Plasma; Poly-fluoroalkyl substances; Predisposition; Prevention strategy; Prospective Studies; Proteins; Research; Research Project Grants; Risk; Risk Factors; Role; Sampling; Seminal; System; Testing; Tissues; United States Environmental Protection Agency; Woman; Work; age related; aged; bead chip; breast cancer diagnosis; cancer diagnosis; cancer initiation; cancer prevention; cancer risk; cancer subtypes; carcinogenicity; case control; cohort; design; environmental chemical; epidemiology study; epigenome-wide association studies; in vivo; innovation; insight; interdisciplinary approach; interest; malignant breast neoplasm; mammary; mammary epithelium; methylome; multidisciplinary; polybrominated diphenyl ether; prospective; stem cells

Today there are more than 85,000 EPA-registered synthetic chemicals, but only 10% have been tested for carcinogenicity in animal studies. Of those tested, ~200 have shown evidence of causing cancer and/or an increase in mammary tumors. However, few of the chemicals have been evaluated in human studies, and results in humans have been inconclusive. Our study will focus on chemical body burden of per- and poly-fluoroalkyl substances (PFAS), and concomitant risk of developing invasive breast cancer during the menopausal transition. These “forever chemicals” are pervasive, enduring, and of high public interest, yet studies of their possible relationship to breast cancer have been limited. We hypothesize that elevated body-burden levels of these endocrine-disrupting chemicals (EDCs) will increase the risk of developing invasive breast cancer, and that alterations in DNA methylation (DNAm) and the breast microenvironment are mechanisms that link these chemical exposures and breast cancer. We will test this hypothesis using a three-pronged approach. In Specific Aim 1, we will conduct a prospective study of women in the American Cancer Society Cancer Prevention Study-3 (CPS-3) to assess the association between body burden of PFAS during the menopausal transition and subsequent development of invasive breast cancer. We will measure PFAS levels in plasma samples collected 1 to 7 years before invasive breast cancer diagnosis in 1000 CPS-3 participants and 1000 matched, cancer-free CPS-3 participants, all between 40 and 57 years of age at blood draw. In Specific Aim 2, studying the same participants, we will measure DNAm using Infinium MethylationEPIC BeadChips and conduct an epigenome- wide association study (EWAS) to identify DNAm changes associated with levels of PFAS in the cancer-free participants. We then will determine the association between the PFAS-associated DNAm changes and risk of developing breast cancer. This valuable DNAm data also can be used later for other outcomes, including exposure to other EDCs. In Specific Aim 3, we will determine the direct effects of PFAS on tissue- and molecular- level states associated with susceptibility to cancer initiation in genomically-well-characterized primary human breast mammary epithelial cells (HMECs). Identifying these mechanisms in primary breast cells is critically important, as PFAS mechanisms of action generally differ by tissue. We will use 2-D cultures to determine the effects of short-term exposures and 3-D cultures to define the effects of protracted chemical exposures on changes in epithelial lineage consistent with accelerated aging and age-related molecular changes in genome methylation, lineage-specific transcription, and cytokeratin proteins. Results from this multi-disciplinary approach will advance our understanding of the effects of PFAS exposures on risk of developing breast cancer during an important window of susceptibility. Ultimately, we hope results from our proposed project will identify an integrated biological signature of environmental exposure, deliver mechanistic insights into breast cancer development from EDCs, and inform future studies for prevention strategies to reduce or mitigate exposures.

如今，有超过85000种在epa注册的合成化学品，但只有10%经过了检测