Flow Cytometry Shared Resources

项目摘要：流式细胞仪共享资源 西尔维斯特综合癌症中心的流式细胞术共享资源(FCSR)是 最先进的设施，提供全面的分析流式细胞仪和高速细胞分选服务 这对许多研究项目的成功进展至关重要。FCSR主任和工作人员的工作 共同提供专业咨询、化验设计、研究培训以及全细胞分析仪 分析和细胞分类服务，以支持研究计划、出版物和拨款申请。这个 FCSR的六台先进的荧光流式细胞仪(三台细胞分析仪和三台细胞分选仪) 促进范围广泛的复杂实验，检验正常和癌症的生物学特性 细胞。FCSR能够同时分析多达19种不同的荧光 参数和两个激光光散射参数(前向和侧向散射) 复杂细胞混合物中细胞内抗原的检测。FCSR执行各种实验分析， 如复杂免疫表型、DNA含量和细胞周期分析、细胞信号和激活 细胞增殖和细胞凋亡的分析和测量。此外，FCSR提供了荧光激活 细胞分选服务，允许调查人员以高纯度和高速提纯散装和单个细胞， 基于复杂的多参数表型。这三个单元格分选器有可能流排序到 17个荧光参数进入散装管或多孔板，用于生物安全下的单细胞流克隆 2级条件。FCSR的三名经验丰富的技术人员执行细胞分选以及连续 对所有仪器进行质量控制评估。他们还提供全面的用户培训，包括 流式细胞仪实验，包括实验设计、荧光试剂板选择、仪器 设置和操作、数据采集和数据分析。对用户进行培训，使他们能够 自己操作分析细胞仪，然后允许在期间或外部独立操作 正常营业时间。用户通过在线仪器预订和发票访问该设施 系统。2017年，FCSR为41名西尔维斯特成员(其中95%是 由同行审查资金支持)，占FCSR总使用量的78%。

PROJECT SUMMARY: FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource (FCSR) at Sylvester Comprehensive Cancer Center (Sylvester) is a state-of-the-art facility offering comprehensive analytical flow cytometry and high-speed cell sorting services that are pivotal to the successful progress of many research projects. The FCSR Director and staff work collectively to provide professional consultation, assay design, and research training as well as full cytometric analysis and cell sorting services to support research programs, publications, and grant applications. The FCSR’s six sophisticated fluorescence flow cytometry instruments (three cell analyzers and three cell sorters) facilitate a wide range of complex experiments examining the biological properties of both normal and cancer cells. The FCSR has the capability to provide simultaneous analysis of up to 19 different fluorescent parameters and two laser light-scatter parameters (forward and side scatter) for both cell surface and intracellular antigen detection in complex cell mixtures. The FCSR performs a variety of experimental analyses, such as complex immunophenotyping, DNA content and cell cycle analysis, cell signaling and activation analysis, and measurement of proliferation and apoptosis. Further, the FCSR provides fluorescence-activated cell sorting services, which allow investigators to purify both bulk and single cells at high purity and high speed, based upon complex multiparametric phenotyping. The three cell sorters have the potential to flow-sort up to 17 fluorescence parameters into bulk tubes or into multi-well plates for single cell flow cloning under Biosafety Level 2 conditions. The FCSR’s three highly experienced technicians perform cell sorting as well as continuous quality control assessment for all instruments. They also provide comprehensive user training in all aspects of flow cytometric experiments, including experimental design, fluorescent reagent panel selection, instrument setup and operation, data acquisition, and data analysis. Users are trained such that they are capable of operating the analysis cytometers themselves and then are permitted to do so independently during or outside regular operating hours. Users access the facility through an online instrument reservation and invoicing system. In 2017, the FCSR provided cytometry services to 41 Sylvester members (95% of whom were supported by peer-reviewed funding), which comprised 78% of total FCSR usage.