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Flow Cytometry Shared Resources

流式细胞术共享资源

基本信息

批准号：
9975822
负责人：
Huw S. Kruger Gray
金额：
$ 22.18万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9975822
关键词：
Activation Analysis Antigens Apoptosis Applications Grants Bioinformatics Shared Resource Biological Biological Assay Biometry Cancer Center Cancer Center Support Grant Cell Cycle Cell Separation Cell surface Cells Clone Cells Cloning Complex Comprehensive Cancer Center Consultations Cytometry Data Analyses Detection Epitopes Experimental Designs Facility Accesses Flow Cytometry Flow Cytometry Shared Resource Fluorescence Fluorescence-Activated Cell Sorting Funding Hour Immunophenotyping Knowledge Label Lasers Measurement Methods Normal Cell Peer Review Phenotype Ploidies Population Preparation Property Publications Quality Control Reagent Research Research Personnel Research Project Grants Research Support Research Training Reservations Resource Sharing Sampling Services Side Signal Transduction Speed Suspensions System Technical Expertise Technology Training Tube Work base cancer cell cancer genomics cell analyzer data acquisition design experience experimental analysis experimental study innovation instrument instrumentation light scattering member novel operation peer support programs single cell analysis

项目摘要

PROJECT SUMMARY: FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource (FCSR) at Sylvester Comprehensive Cancer Center (Sylvester) is a state-of-the-art facility offering comprehensive analytical flow cytometry and high-speed cell sorting services that are pivotal to the successful progress of many research projects. The FCSR Director and staff work collectively to provide professional consultation, assay design, and research training as well as full cytometric analysis and cell sorting services to support research programs, publications, and grant applications. The FCSR’s six sophisticated fluorescence flow cytometry instruments (three cell analyzers and three cell sorters) facilitate a wide range of complex experiments examining the biological properties of both normal and cancer cells. The FCSR has the capability to provide simultaneous analysis of up to 19 different fluorescent parameters and two laser light-scatter parameters (forward and side scatter) for both cell surface and intracellular antigen detection in complex cell mixtures. The FCSR performs a variety of experimental analyses, such as complex immunophenotyping, DNA content and cell cycle analysis, cell signaling and activation analysis, and measurement of proliferation and apoptosis. Further, the FCSR provides fluorescence-activated cell sorting services, which allow investigators to purify both bulk and single cells at high purity and high speed, based upon complex multiparametric phenotyping. The three cell sorters have the potential to flow-sort up to 17 fluorescence parameters into bulk tubes or into multi-well plates for single cell flow cloning under Biosafety Level 2 conditions. The FCSR’s three highly experienced technicians perform cell sorting as well as continuous quality control assessment for all instruments. They also provide comprehensive user training in all aspects of flow cytometric experiments, including experimental design, fluorescent reagent panel selection, instrument setup and operation, data acquisition, and data analysis. Users are trained such that they are capable of operating the analysis cytometers themselves and then are permitted to do so independently during or outside regular operating hours. Users access the facility through an online instrument reservation and invoicing system. In 2017, the FCSR provided cytometry services to 41 Sylvester members (95% of whom were supported by peer-reviewed funding), which comprised 78% of total FCSR usage.

项目总结：流式细胞术共享资源