Characterizing the Metabolome and Volatilome of Gut Microbiome

表征肠道微生物组的代谢组和挥发物组

• 批准号: 10681064
• 负责人: Jiangjiang Zhu
• 金额: $ 22.78万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10681064
• 关键词: Area; Behavior; Biological; Biotechnology; Blood Vessels; Catechin; Detection; Development; Dietary Component; Dietary Intervention; Disease; Electrospray Ionization; Energy Metabolism; Guidelines; Health; Homeostasis; Human; Immune; In Vitro; Individual; Intervention; Investigation; Knowledge; Mass Spectrum Analysis; Measurement; Metabolic; Metabolism; Methods; Modeling; Molecular; Neurologic; Obesity; Physiological Processes; Physiology; Play; Research; Resolution; Role; Sampling; Spectrometry; Techniques; Therapeutic; Therapeutic Intervention; base; functional food; gut microbiome; host microbiome; ion mobility; metabolome; metabolomics; microbial; microbial community; novel; nutrition; programs; rapid detection; tool; volatile organic compound

Project Summary: Previous studies have shown that microbial metabolites are often the compounds most markedly altered in the disease state when comparing diseased versus healthy individuals. Recent studies also suggest that metabolites deriving from microbial transformation of dietary components have significant effects on host physiological processes such as energy metabolism, gut and immune homeostasis, neurological behavior, and vascular function. Additionally, it has been proposed that metabolomics-directed functional interventions using exogenous metabolites may play critical biological roles in cellular activities. However, there are no existing, detailed, and quantitative techniques for the measurement of a broad range of endogenous microbial metabolites, nor has there been a systematic investigation of microbial communities for their metabolic functions. To fill the existing gap of knowledge, the research program outlined in this proposal focuses on three core areas: 1) Develop advanced mass spectrometry-based metabolomics technique for sensitive, quantitative detection and broad coverage of gut microbial metabolites; 2) Develop Secondary Electrospray Ionization – Field Asymmetric Waveform Ion Mobility Spectrometry – High-Resolution Mass Spectrometry (SESI-FAIMS- HRMS) for the rapid detection of gut microbial volatile organic compound detection; 3) Utilizing existing samples from our collaborator's lab to validate our metabolomics methods and elucidate the functionality of catechins microbial metabolites in obese host. These three coherent areas center on the development and application of mass spectrometry-based metabolomics, volatilomics and related microbial biotechnology tools to enable functional studies of microbial metabolism and nutrition-gut microbiome-host interactions. We believe this research program has great potential to elucidate the impact of microbial metabolites on host health and diseases, and to provide guidelines for modulating these metabolites to realize the full therapeutic potential, and for targeting microbial metabolism to promote human health.

项目概要： 先前的研究表明，微生物代谢物通常是环境中改变最显着的化合物。 比较患病个体与健康个体时的疾病状态。最近的研究还表明 膳食成分微生物转化产生的代谢产物对宿主有显着影响 生理过程，如能量代谢、肠道和免疫稳态、神经行为和 血管功能。此外，有人提出，代谢组学指导的功能干预使用 外源代谢物可能在细胞活动中发挥关键的生物学作用。然而，目前还没有现成的、 用于测量各种内源微生物的详细定量技术 代谢物，也没有对微生物群落的代谢进行系统研究 功能。为了填补现有的知识空白，本提案中概述的研究计划重点关注三个方面 核心领域： 1) 开发先进的基于质谱的代谢组学技术，以实现灵敏、定量 肠道微生物代谢物的检测和广泛覆盖； 2) 开发二次电喷雾电离 – 场不对称波形离子淌度谱分析 – 高分辨率质谱分析 (SESI-FAIMS- HRMS）用于快速检测肠道微生物挥发性有机化合物检测； 3）利用现有的 来自我们合作者实验室的样本，用于验证我们的代谢组学方法并阐明其功能 儿茶素是肥胖宿主体内的微生物代谢产物。这三个相关领域集中于发展和 基于质谱的代谢组学、挥发组学和相关微生物生物技术工具的应用 实现微生物代谢和营养-肠道微生物组-宿主相互作用的功能研究。我们相信 该研究计划具有阐明微生物代谢物对宿主健康和 疾病，并为调节这些代谢物以实现全部治疗潜力提供指导， 并针对微生物代谢来促进人类健康。