Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围

• 批准号: 10674990
• 负责人: Viviana Gradinaru
• 金额: $ 48.92万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674990
• 关键词: Adopted; African American student; BRAIN initiative; Biological; Biological Assay; Biotechnology; Brain; California; Capsid; Cataloging; Cell Culture Techniques; Collaborations; Communities; Custom; Dedications; Dependovirus; Disease; Diverse Workforce; Dose; Ecosystem; Education; Educational workshop; Electronics; Eligibility Determination; Engineering; Ensure; Equipment and supply inventories; Equity; Feedback; Fees; Fostering; Freezing; Funding; Gene Delivery; Generations; Genome; Hispanic-serving Institution; Human; Immunohistochemistry; Infrastructure; Injections; Link; Mammalian Cell; Mentors; Methodology; Methods; Minority-Serving Institution; Mission; Modernization; Molecular; Monitor; Mus; Neuromodulator; Neurons; Neurosciences; Population; Process; Production; Publications; Quality Control; Reagent; Research; Research Personnel; Resources; Running; Science; Scientist; Site; Structure; Students; Support System; System; Training; Tropism; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Virus; Work; adeno-associated viral vector; aluminum sulfate; career; cell type; experience; gene therapy; innovation; instrumentation; meetings; next generation; novel; posters; pre-doctoral; programs; quality assurance; science and society; sensor; sex; summer program; tool; undergraduate research; undergraduate student; vector; virus identification; web site

PROJECT SUMMARY The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Miles), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Miles) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目总结 AAV大脑安全有效的神经调节剂和传感器跨物种利用(由领导的人口普查 加州理工学院)将开发、验证和传播完整的 (衣壳和基因组)工程化腺相关病毒(AAV)工具用于监测和操纵分子 定义了脊椎动物物种中的神经细胞类型。为了扩大这些试剂的使用范围 神经科学界，我们建议AAV脑普查和加利福尼亚州建立密切合作伙伴关系 波莫纳州立理工大学(CPP)，它将拥有一个新的武器载体核心(ARMVC)。这 协作还将增加未被充分代表的少数群体(URM)进入研究社区本身的机会 和少数族裔服务机构(MSI)，因为CPP是指定的拉美裔服务机构，也为大型 符合佩尔资格的第一代和非裔美国学生的群体。这一伙伴关系将促进 AAV神经科学工程专家之间的交流(Pi Gradinaru)，AAV生产创新 和传播(Pi Miles)，以及URM本科生研究和指导(Co-I Steele)建立物理 和人力基础设施，用于CPP的AAV生产、验证和传播，同时全面 整合MSI本科生。 加州理工学院的三叶草中心(由Pi Miles指导)将利用他们运行成功的AAV的经验 传播中心与Co-I Steele和Damien Wolfe，M.S.，CPP和Steele Lab Alum and Clover合作 将在ArmVC工作的技术人员，以建立强大的AAV生产和传播能力 CPP的ArmVC(目标1)。克洛弗和ArmVC将合作运行一项新的AAV脑普查计划 包容性研究体验(ASPIRE)，其中CPP本科生被选为有抱负的科学家 将制作有效的AAV，作为新的ARMVC的一部分传播给神经科学界(AIM 2) 并在Co-I Steele的实验室工作，使用免疫组织化学独立验证鉴定出的新型AAV 通过PI Gradinaru实验室的高通量方法(目标3)。Aspire科学家将从以下活动中受益 与Pi Gradinaru一起举办研讨会、海报演示、研究研讨会和以职业为重点的指导会议 在CPP和加州理工学院工作。 三叶草年度AAV生产培训(以及对返乡员工和本科生的再培训) 工作人员将确保在AAV生产、纯化和表征方面取得方法学上的进步 或被三叶草中心采用的电子邮件在不同地点之间迅速转移。ArmVC产品将进行宣传，并 通过独立网站传播，同时也集成到AAV脑普查电子系统中 用于无缝试剂编目和反馈的资源。具有强大的质量控制和质量保证 流程到位并由克洛弗监督，CPP的ArmVC将拓宽接触现代神经科学的途径 工具和帮助弥合URM在研究科学的职业发展方面的差距。