Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围

基本信息

批准号：
10674990
负责人：
Viviana Gradinaru
金额：
$ 48.92万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10674990
关键词：
Adopted African American student BRAIN initiative Biological Biological Assay Biotechnology Brain California Capsid Cataloging Cell Culture Techniques Collaborations Communities Custom Dedications Dependovirus Disease Diverse Workforce Dose Ecosystem Education Educational workshop Electronics Eligibility Determination Engineering Ensure Equipment and supply inventories Equity Feedback Fees Fostering Freezing Funding Gene Delivery Generations Genome Hispanic-serving Institution Human Immunohistochemistry Infrastructure Injections Link Mammalian Cell Mentors Methodology Methods Minority-Serving Institution Mission Modernization Molecular Monitor Mus Neuromodulator Neurons Neurosciences Population Process Production Publications Quality Control Reagent Research Research Personnel Resources Running Science Scientist Site Structure Students Support System System Training Tropism Underrepresented Minority United States National Institutes of Health Universities Validation Virus Work adeno-associated viral vector aluminum sulfate career cell type experience gene therapy innovation instrumentation meetings next generation novel posters pre-doctoral programs quality assurance science and society sensor sex summer program tool undergraduate research undergraduate student vector virus identification web site

项目摘要

PROJECT SUMMARY The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Miles), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Miles) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目摘要 AAV大脑安全有效的神经调节剂和跨物种的传感器利用（Sensus，由 NIH大脑倡议Armammentarium项目的Caltech）将开发，验证和传播综合的（CAPSID和基因组）设计的腺相关病毒（AAV）工具以监测和操纵分子跨脊椎动物的定义神经元细胞类型。扩大对这些试剂的访问神经科学社区，我们提出了AAV Brain Sensus与加利福尼亚州之间的密切合作伙伴关系州理工大学，波莫纳（CPP），该大学将举办新的Armammentarium Vector Core（ARMVC）。这合作还将增加对代表性不足的少数民族（URM）的研究社区本身的访问权限以及少数族裔服务机构（MSI），因为CPP是一个指定的西班牙裔服务机构，也为大型服务符合佩尔资格，第一代和非裔美国学生的人群。这种伙伴关系将促进关闭 AAV工程专家（PI Gradinaru），AAV生产创新和传播（PI英里），以及URM的本科研究和心理（Co-I Steele）来建立身体以及在CPP上进行AAV生产，验证和传播的人类基础设施，同时全面整合MSI大学生。加州理工学院的三叶草中心（由PI Miles执导）将利用他们在成功的AAV上的经验与Co-i Steele和Damien Wolfe，M.S。，CPP和Steele Lab Alum和Clover合作技术谁将在ARMVC工作，以建立强大的AAV生产和传播能力 ARMVC在CPP（AIM 1）。三叶草和ARMVC将合作运行一个新的AAV脑感知程序包容性研究经验（ASPIRE），其中CPP本科生选择为Aspire科学家作为新ARMVC的一部分，将产生经过验证的AAV向神经科学社区传播（AIM 2）并在Co-i Steele的实验室工作，使用免疫组织化学独立验证小说AAV确定通过Pi Gradinaru实验室中的高通量方法（AIM 3）。 Aspire科学家将受益于包括与PI Gradinaru的讲习班，海报演示，研究中心和以职业为中心的心理会议在CPP和Caltech。三叶草的年度培训（并重新培训返回员工和本科生）员工将确保开发AAV生产，纯化和表征的方法论进步或三叶草中心采用的迅速在站点之间转移。 ARMVC产品将被宣传，并且通过独立网站传播，同时也集成到AAV脑Sensus电子中无缝试剂编目和反馈的资源。具有强大的质量控制和质量保证 CPP的ARMVC将扩大对现代神经科学的访问权限，并由三叶草进行的过程和监督工具并有助于弥合URM研究科学职业发展的差距。