Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围

• 批准号: 10674990
• 负责人: Viviana Gradinaru
• 金额: $ 48.92万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674990
• 关键词: Adopted; African American student; BRAIN initiative; Biological; Biological Assay; Biotechnology; Brain; California; Capsid; Cataloging; Cell Culture Techniques; Collaborations; Communities; Custom; Dedications; Dependovirus; Disease; Diverse Workforce; Dose; Ecosystem; Education; Educational workshop; Electronics; Eligibility Determination; Engineering; Ensure; Equipment and supply inventories; Equity; Feedback; Fees; Fostering; Freezing; Funding; Gene Delivery; Generations; Genome; Hispanic-serving Institution; Human; Immunohistochemistry; Infrastructure; Injections; Link; Mammalian Cell; Mentors; Methodology; Methods; Minority-Serving Institution; Mission; Modernization; Molecular; Monitor; Mus; Neuromodulator; Neurons; Neurosciences; Population; Process; Production; Publications; Quality Control; Reagent; Research; Research Personnel; Resources; Running; Science; Scientist; Site; Structure; Students; Support System; System; Training; Tropism; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Virus; Work; adeno-associated viral vector; aluminum sulfate; career; cell type; experience; gene therapy; innovation; instrumentation; meetings; next generation; novel; posters; pre-doctoral; programs; quality assurance; science and society; sensor; sex; summer program; tool; undergraduate research; undergraduate student; vector; virus identification; web site

PROJECT SUMMARY The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Miles), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Miles) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目摘要 AAV BRAIN跨物种安全有效的神经调节剂和传感器利用（SENSUS，由 美国国立卫生研究院脑倡议军备项目的加州理工学院）将开发，验证和传播集成 （衣壳和基因组）工程腺相关病毒（AAV）工具，以监测和操纵分子 在脊椎动物物种中定义了神经元细胞类型。为了扩大这些试剂的使用范围， 神经科学界，我们建议AAV大脑感觉和加州之间的密切合作关系 波莫纳州立理工大学（CPP），将主办一个新的Armamentarium矢量核心（ArmVC）。这 合作还将增加代表性不足的少数民族（URM）进入研究界的机会 和少数民族服务机构（MSI），因为CPP是一个指定的西班牙裔服务机构，也为大型 佩尔合格，第一代和非裔美国学生的人口。这种合作关系将促进密切的 神经科学AAV工程专家（PI Gradinaru），AAV生产创新 和传播（PI英里），和URM本科研究和指导（Co-I斯蒂尔），以建立物理 和人力基础设施的AAV生产，验证和传播在CPP，同时全面 整合MSI本科生。 加州理工学院的三叶草中心（由PI迈尔斯指导）将利用他们的经验，在运行一个成功的AAV 传播中心与Co-I Steele和Damien Wolfe，M.S.，CPP和斯蒂尔实验室明矾和三叶草 技术人员将在ArmVC工作，以建立强大的AAV生产和传播能力， CPP（目标1）处的ArmVC。三叶草和ArmVC将合作运行一个新的AAV大脑感知计划， 包容性研究经验（ASPIRE），其中CPP本科生被选为ASPIRE科学家 将生产经过验证的AAV，作为新的ArmVC的一部分分发给神经科学界（Aim 2） 并在Co-I Steele的实验室工作，使用免疫组织化学独立验证新的AAV鉴定 通过PI Gradinaru实验室的高通量方法（Aim 3）。ASPIRE科学家将受益于以下活动， 与PI Gradinaru一起举办研讨会，海报展示，研究研讨会和以职业为中心的指导会议 在CPP和加州理工学院 由三叶草进行AAV生产的年度培训（以及对返回工作人员和本科生的再培训） 工作人员将确保在AAV生产、纯化和表征方面的方法进步 或被三叶草中心采用的植物在不同地点之间迅速转移。ArmVC产品将被公布， 通过一个独立的网站传播，同时也被集成到AAV BRAIN SENSUS电子 用于无缝试剂编目和反馈的资源。拥有强大的质量控制和质量保证 在三叶草的监督下，CPP的ArmVC将拓宽现代神经科学的途径， 工具和帮助弥合差距在URM研究科学的职业发展。