Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences - SUPPLEMENT

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围 - 补充

• 批准号: 10885509
• 负责人: Viviana Gradinaru
• 金额: $ 15.57万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10885509
• 关键词: Adopted; African American student; Award; BRAIN initiative; Biological; Biological Assay; Biotechnology; Brain; California; Capsid; Cataloging; Cell Culture Techniques; Collaborations; Communities; Custom; Dedications; Dependovirus; Disease; Diverse Workforce; Dose; Ecosystem; Education; Educational workshop; Electronics; Eligibility Determination; Engineering; Ensure; Equipment and supply inventories; Equity; Feedback; Fees; Fostering; Freezing; Funding; Gene Delivery; Generations; Genome; Hispanic-serving Institution; Human; Immunohistochemistry; Infrastructure; Injections; Link; Mammalian Cell; Mentors; Methodology; Methods; Minority-Serving Institution; Mission; Modernization; Molecular; Monitor; Mus; Neuromodulator; Neurons; Neurosciences; Population; Process; Production; Publications; Quality Control; Reagent; Research; Research Personnel; Resources; Running; Science; Scientist; Site; Structure; Students; Support System; System; Training; Tropism; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Virus; Work; adeno-associated viral vector; aluminum sulfate; career; cell type; experience; gene therapy; innovation; instrumentation; meetings; next generation; novel; parent grant; posters; pre-doctoral; programs; quality assurance; science and society; sensor; sex; summer program; tool; undergraduate research; undergraduate student; vector; virus identification; web site

PROJECT SUMMARY (Changes from awarded parent grant in bold) The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Shay), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Shay) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目摘要（由已获发的家长补助金更改的粗体）

项目成果

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.

• DOI: 10.1126/sciadv.adg6618
• 发表时间: 2023-04-21
• 期刊: SCIENCE ADVANCES
• 影响因子: 13.6
• 作者: Shay, Timothy F.;Sullivan, Erin E.;Ding, Xiaozhe;Chen, Xinhong;Kumar, Sripriya Ravindra;Goertsen, David;Brown, David;Crosby, Anaya;Vielmetter, Jost;Wolfe, Damien A.;Lam, Annie W.;Gradinaru, Viviana
• 通讯作者: Gradinaru, Viviana