Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences - SUPPLEMENT

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围 - 补充

• 批准号: 10885509
• 负责人: Viviana Gradinaru
• 金额: $ 15.57万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10885509
• 关键词: Adopted; African American student; Award; BRAIN initiative; Biological; Biological Assay; Biotechnology; Brain; California; Capsid; Cataloging; Cell Culture Techniques; Collaborations; Communities; Custom; Dedications; Dependovirus; Disease; Diverse Workforce; Dose; Ecosystem; Education; Educational workshop; Electronics; Eligibility Determination; Engineering; Ensure; Equipment and supply inventories; Equity; Feedback; Fees; Fostering; Freezing; Funding; Gene Delivery; Generations; Genome; Hispanic-serving Institution; Human; Immunohistochemistry; Infrastructure; Injections; Link; Mammalian Cell; Mentors; Methodology; Methods; Minority-Serving Institution; Mission; Modernization; Molecular; Monitor; Mus; Neuromodulator; Neurons; Neurosciences; Population; Process; Production; Publications; Quality Control; Reagent; Research; Research Personnel; Resources; Running; Science; Scientist; Site; Structure; Students; Support System; System; Training; Tropism; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Virus; Work; adeno-associated viral vector; aluminum sulfate; career; cell type; experience; gene therapy; innovation; instrumentation; meetings; next generation; novel; parent grant; posters; pre-doctoral; programs; quality assurance; science and society; sensor; sex; summer program; tool; undergraduate research; undergraduate student; vector; virus identification; web site

PROJECT SUMMARY (Changes from awarded parent grant in bold) The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Shay), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Shay) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目摘要（BOLD授予的家长赠款的更改） AAV大脑安全有效的神经调节剂和跨物种的传感器利用（Sensus，由 NIH大脑倡议Armammentarium项目的Caltech）将开发，验证和传播综合的 （CAPSID和基因组）设计的腺相关病毒（AAV）工具以监测和操纵分子 跨脊椎动物的定义神经元细胞类型。扩大对这些试剂的访问 神经科学社区，我们提出了AAV Brain Sensus与加利福尼亚州之间的密切合作伙伴关系 州理工大学，波莫纳（CPP），该大学将举办新的Armammentarium Vector Core（ARMVC）。这 合作还将增加对代表性不足的少数民族（URM）的研究社区本身的访问权限 以及少数族裔服务机构（MSI），因为CPP是一个指定的西班牙裔服务机构，也为大型服务 符合佩尔资格，第一代和非裔美国学生的人群。这种伙伴关系将促进关闭 AAV工程专家（PI Gradinaru），AAV生产创新 和传播（PI Shay）和URM本科研究和心理（Co-I Steele）来建立身体 以及在CPP上进行AAV生产，验证和传播的人类基础设施，同时全面 整合MSI大学生。 加州理工学院的三叶草中心（由PI Shay执导）将利用他们成功的AAV经验 与Co-i Steele和Damien Wolfe，M.S。，CPP和Steele Lab Alum和Clover合作 技术谁将在ARMVC工作，以建立强大的AAV生产和传播能力 ARMVC在CPP（AIM 1）。三叶草和ARMVC将合作运行一个新的AAV脑感知程序 包容性研究经验（ASPIRE），其中CPP本科生选择为Aspire科学家 作为新ARMVC的一部分，将产生经过验证的AAV向神经科学社区传播（AIM 2） 并在Co-i Steele的实验室工作，使用免疫组织化学独立验证小说AAV确定 通过Pi Gradinaru实验室中的高通量方法（AIM 3）。 Aspire科学家将受益于包括 与PI Gradinaru的讲习班，海报演示，研究中心和以职业为中心的心理会议 在CPP和Caltech。 三叶草的年度培训（并重新培训返回员工和本科生） 员工将确保开发AAV生产，纯化和表征的方法论进步 或三叶草中心采用的迅速在站点之间转移。 ARMVC产品将被宣传，并且 通过独立网站传播，同时也集成到AAV脑Sensus电子中 无缝试剂编目和反馈的资源。具有强大的质量控制和质量保证 CPP的ARMVC将扩大对现代神经科学的访问权限，并由三叶草进行的过程和监督 工具并有助于弥合URM研究科学职业发展的差距。

项目成果

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.

• DOI: 10.1126/sciadv.adg6618
• 发表时间: 2023-04-21
• 期刊: SCIENCE ADVANCES
• 影响因子: 13.6
• 作者: Shay, Timothy F.;Sullivan, Erin E.;Ding, Xiaozhe;Chen, Xinhong;Kumar, Sripriya Ravindra;Goertsen, David;Brown, David;Crosby, Anaya;Vielmetter, Jost;Wolfe, Damien A.;Lam, Annie W.;Gradinaru, Viviana
• 通讯作者: Gradinaru, Viviana