Einstein's Nathan Shock Center of Excellence in Basic Biology of Aging

爱因斯坦内森休克衰老基础生物学卓越中心

• 批准号: 10675109
• 负责人: ANA MARIA CUERVO
• 金额: $ 21.77万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10675109
• 关键词: Address; Advisory Committees; Age; Aging; Applications Grants; Autophagocytosis; Biochemical; Biological Assay; Biology of Aging; Cells; Chemicals; Communication; Communities; Consultations; Cost efficiency; Data; Data Analyses; Development; Disease; Drug Design; Educational workshop; Electron Microscopy; Equipment; Experimental Designs; Experimental Models; Fee-for-Service Plans; Funding; Future; Homeostasis; Human; Human Resources; Image; Individual; Inflammation; Institution; International; Intervention; Invertebrates; Knowledge; Life; Link; Maintenance; Mammals; Metabolism; Methodological Studies; Methodology; Methods; Molecular; Molecular Chaperones; Morphology; Natural Compound; Organism; Pathway interactions; Pattern; Peer Review; Pharmaceutical Chemistry; Pharmaceutical Preparations; Phenotype; Preparation; Procedures; Process; Protocols documentation; Publications; Quality Control; Reagent; Research; Research Personnel; Research Support; Resources; Sampling; Services; Shock; Standardization; Stress; System; Techniques; Technology; Testing; Tissues; Training; Translations; Treatment Efficacy; Validation; Work; age related; catalyst; design; drug development; gene therapy; hands-on learning; healthspan; innovation; interest; learning strategy; meetings; member; microscopic imaging; multicatalytic endopeptidase complex; novel; operation; prevent; programs; protein degradation; proteostasis; response; stem cell function; therapy design; tool

Summary The Proteostasis of Aging Core (PAC) is a resource for investigators interested in the study of changes in proteostasis in aging and age-related disorders at the Einstein-Nathan Shock Center (E-NSC) and elsewhere by providing state-of-the-art methodology for the study of changes in components of the proteostasis network. The PAC has provided 917 services for 105 groups worldwide, contributed to 42 publications, generated data for 23 grant applications and catalyzed numerous collaborative projects. The Specific Aims of this Core are to: 1) perform comprehensive and highly specialized assays for the study of autophagy and other proteostasis components; 2) distribute validated reagents, samples and protocols for the study of proteostasis; 3) provide advice on experimental design, techniques, procedures and data interpretation for proteostasis analysis and for development of proteostasis targeting interventions; 4) develop and adapt state-of-the-art methodology for the study of proteostasis; 5) contribute to training of aging investigators in methods and procedures for the study of proteostasis, and 5) become a resource for information in proteostasis through integration with the NSC coordinating center and with information from other NSC cores. The activities of the Core are organized into four groups: 1) Service: comprehensive battery of assays to study protein turnover, activities of the different autophagic pathways and proteasome and lysosomal function in general. 2) Resources: provides validated reagents, samples and protocols for the study of proteostasis in aging. 3) Consultation and training: includes meetings with PAC experts for experimental planning, data interpretation and drug design, and hands-on training sessions with the technician. 4) Innovation: explores, tests and implements procedures for the study of protein homeostasis with emphasis on autophagic pathways. Personnel: 1) Director (Dr. Cuervo) supervises all activities of the Core, offers advice, assists with results interpretation and communicates with the Advisory Committee to establish prioritization/ workflow patterns. 2) Two experts provide advice on proteostasis, perform morphometric analysis and supervises implementation of new procedures and provide advice on drug design, experimental design of interventions and interpretation of results. 3) The PAC technician performs assays offered as a service, contributes to training and assures maintenance of equipment and stocks. Relevance: Unique services and continuous actualization of technology and resources offered by the Core allow for comprehensive quantitative analysis of changes in proteostasis in aging, age-related disorders and the of the effect of different interventions. Centralization of procedures and resources optimizes cost efficiency and guarantees standardization, validation and tight quality control.

总结 蛋白质稳定老化核心（PAC）是一个资源，为研究人员感兴趣的变化， 在爱因斯坦-内森休克中心（E-NSC）和其他地方， 提供了最先进的方法学的变化的研究组成部分的蛋白质平衡网络。的 PAC为全球105个团体提供了917项服务，为42份出版物供稿，为23个团体提供了数据。 资助申请并促成了许多合作项目。该核心的具体目标是：1） 进行全面和高度专业化的分析，用于研究自噬和其他蛋白质稳定 组件; 2）分发经验证的试剂，样品和蛋白质抑制研究方案; 3）提供 就蛋白质抑制分析和蛋白质合成的实验设计、技术、程序和数据解释提供建议， 开发蛋白质代谢抑制靶向干预措施; 4）开发和调整最先进的方法， 研究蛋白质稳态; 5）有助于培训老龄化研究人员的方法和程序的研究， 蛋白质稳定，和5）通过与NSC整合成为蛋白质稳定的信息资源 协调中心和来自其他NSC核心的信息。 核心的活动分为四组：1）服务：全面的分析， 研究蛋白质周转，不同自噬途径的活性以及蛋白酶体和溶酶体的功能， 将军2)资源：提供经验证的试剂，样品和方案，用于蛋白质代谢抑制的研究。 衰老3)咨询和培训：包括与PAC专家就实验规划、数据 解释和药物设计，以及与技术人员的实践培训课程。4)创新：探索， 测试和实施蛋白质稳态研究程序，重点是自噬途径。 人事：1）主任（Cuervo博士）监督核心的所有活动，提供建议，协助取得成果 该委员会负责口译工作，并与行预咨委会沟通，以确定优先次序/工作流程模式。（二） 两名专家提供蛋白酶抑制方面的建议，进行形态学分析，并监督 新的程序，并提供有关药物设计，干预措施的实验设计和解释的建议， 结果3)PAC技术人员执行作为服务提供的分析，参与培训并确保 设备和库存的维护。相关性：独特的服务和技术的持续实现 和资源提供的核心允许全面定量分析的变化，蛋白质稳态， 老龄化、与年龄有关的疾病以及不同干预措施的效果。程序的集中化， 资源优化了成本效率，并保证了标准化、验证和严格的质量控制。