Imaging and Surgery Core

影像和手术核心

• 批准号: 10674672
• 负责人: Gwendalyn J Randolph
• 金额: $ 45.09万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674672
• 关键词: Antibodies; Behavior; Biology; Blood Vessels; Blood flow; Brain region; Cells; Collaborations; Color; Coupled; Data; Data Analyses; Data Set; Diameter; Erythrocytes; Grant; High Density Lipoproteins; Image; Image Analysis; Image-Guided Surgery; Immune; Individual; Laboratories; Lipoproteins; Lymphatic; Macrophage; Meningeal; Meninges; Methods; Modality; Molecular; Natural Immunity; Operative Surgical Procedures; Oxygen; Pathogenesis; Pathology; Penetration; Procedures; Reagent; Refractory; Research; Research Personnel; Resolution; Role; Sampling; Science; Services; Speed; Stains; Standardization; Surgeon; Technology; Thinness; Three-Dimensional Imaging; Tissue imaging; Tissues; Tracer; Transplantation; combinatorial; cranium; experience; improved; indexing; innovation; intravital imaging; light microscopy; lymphatic vasculature; mouse model; neuroimmunology; novel; oxygen transport; photoacoustic imaging; programs; protocol development; skills; tissue fixing; tool; trafficking; two photon microscopy; two-photon

ABSTRACT – Core B The imaging and surgical core will provide state-of-the-art imaging services to the program. Dr. Randolph’s experience in organizing collaborative teams and her laboratory's background in macrophage biology and lymphatic/vascular trafficking of immune cells and lipoproteins will facilitate applications in imaging that will be tailored to the science of each project. The core will incorporate major contributions from highly expert co- investigators. Dr. Bernd Zinselmeyer is an expert in multiple modalities of light microscopy, including its application in studies of the CNS. He will contribute several lines of expertise in the grant, from confocal and 2- photon microscopy to high-speed epifluorescence imaging. Dr. Song Hu is an innovator in photoacoustic imaging with demonstrated expertise in using this technology to study the vasculature of the CNS. Dr. Igor Smirnov is an expert microsurgeon who will provide and oversee highly skilled surgical manipulations for the projects, including procedures like skull transplant and skull thinning. These co-investigators bring more than their state-of-the-art expertise; they also all bring demonstrated collaborations with one or more project leaders. By working together, this core team of investigators and affiliated staff will offer to all projects the following range of expert surgical procedures and imaging applications. The aims of the imaging and surgical core are to (1) carry out deep-tissue imaging of meninges and outer regions of the brain, (2) apply combinatorial methods (2-photon, high-speed widefield, and photoacoustic imaging) for intravital imaging to investigate macrophage behavior and blood flow dynamics in the meninges, and (3) employ established and novel tools to quantify molecular outflow from the meningeal space.

摘要-核心B 成像和外科核心将为该计划提供最先进的成像服务。兰多夫医生 组织协作团队的经验和她的实验室在巨噬细胞生物学方面的背景， 免疫细胞和脂蛋白的淋巴/血管运输将促进成像中的应用， 为每个项目的科学性量身定制。核心部分将吸收高度专业的共同- investigators. Bernd Zinselmeyer博士是多种光学显微镜模式的专家，包括其 在CNS研究中的应用。他将在赠款中贡献几条专业知识线，从共聚焦和2- 光子显微镜到高速落射荧光成像。宋虎博士是光声成像领域的创新者 在使用该技术研究CNS血管系统方面具有专业知识。伊戈尔·斯米尔诺夫博士是 专家显微外科医生谁将提供和监督高技能的手术操作的项目，包括 比如颅骨移植和颅骨减薄这些合作研究者带来的不仅仅是他们最先进的技术 专业知识;他们也都与一个或多个项目负责人进行了合作。通过合作， 这支由调查人员和附属工作人员组成的核心团队将为所有项目提供以下范围的专家手术 程序和成像应用。影像学和外科学的核心目标是（1）进行深层组织 脑膜和大脑外部区域的成像，（2）应用组合方法（2-光子，高速 宽视野和光声成像）进行活体成像，以研究巨噬细胞行为和血流 动力学在脑膜，和（3）采用既定的和新的工具，以量化分子流出从 脑膜间隙