CRE MOUSE MODELS TO STUDY AMELOGENESIS

研究釉质形成的 CRE 小鼠模型

基本信息

项目摘要

PROJECT SUMMARY / ABSTRACT In June of 2017 a group of dental researchers met at the NIDCR to discuss the future of enamel research and concluded, in a summary statement, that in the field of enamel research, a lack of models to study enamel formation and disease has hampered recent progress. The group concluded that more appropriate ameloblast- like cell lines, investment in organoid and chip technology and novel animal models could be used to advance the field. With respect to animal models, I believe enamel researchers suffer from not having enamel organ- specific Cre recombinase mutant mouse. For the past 2 decades enamel researchers have used the Krt14-Cre (keratin 14-Cre recombinase) mutant to study enamel-specific activities by cross breeding with various loxP mouse lines. The significant disadvantage of using the Krt14-Cre mouse for enamel research is that Krt14 is expressed in multiple tissues including skin, bronchial epithelia, tongue, trachea, salivary glands, and many more organs, and because of this many of the developed loxP mouse lines are not appropriate to study amelogenesis. For example, mRNA expression levels of the anion exchanger protein (Slc4a2/AE2), or the cystic fibrosis transmembrane conductance regulator (Cftr), increases ~ 6-fold and 3-fold respectively in the enamel organ during maturation stage (compared to secretory stage), and beyond tooth formation both genes are widely expressed in lung and pancreas and are critical to their development. Both Slc4a2-null and Cftr-null mice have severe enamel pathologies. To use the Krt14-Cre mutant mouse to study the role of either AE2fl/fl or Cftrfl/fl would have significant limitations because these animals would predictably suffer from multiple organ failures at a young age. I propose to develop two animal models with a knockin of Cre recombinase into the ameloblastin (Ambn) and odontogenic ameloblast-associated (Odam) gene loci such that Cre expression is limited to secretory ameloblasts and maturation ameloblasts respectively. The UG3 stage of this grant will be devoted to the development of these two animal models; Ambn-Cre and Odam-Cre, and the UH3 phase will validate these two animals as unique in vivo models to study amelogenesis. At the completion of this project data from these animals will be published, and both lines deposited in an appropriate facility such as the Mutamt Mouse Resource and Research Centers (MMRRC).
项目摘要/摘要

项目成果

期刊论文数量(0)
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会议论文数量(0)
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Michael Lansdell Paine其他文献

Michael Lansdell Paine的其他文献

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{{ truncateString('Michael Lansdell Paine', 18)}}的其他基金

CRE MOUSE MODELS TO STUDY AMELOGENESIS
研究釉质形成的 CRE 小鼠模型
  • 批准号:
    10460289
  • 财政年份:
    2021
  • 资助金额:
    $ 41.25万
  • 项目类别:
Amelogenesis and Ion Transport
釉质生成和离子运输
  • 批准号:
    10570193
  • 财政年份:
    2021
  • 资助金额:
    $ 41.25万
  • 项目类别:
Amelogenesis and Ion Transport
釉质生成和离子运输
  • 批准号:
    10193340
  • 财政年份:
    2021
  • 资助金额:
    $ 41.25万
  • 项目类别:
CRE MOUSE MODELS TO STUDY AMELOGENESIS
研究釉质形成的 CRE 小鼠模型
  • 批准号:
    10416106
  • 财政年份:
    2021
  • 资助金额:
    $ 41.25万
  • 项目类别:
Amelogenesis and Ion Transport
釉质生成和离子运输
  • 批准号:
    10372197
  • 财政年份:
    2021
  • 资助金额:
    $ 41.25万
  • 项目类别:
CRE MOUSE MODELS TO STUDY AMELOGENESIS
研究釉质形成的 CRE 小鼠模型
  • 批准号:
    9796804
  • 财政年份:
    2019
  • 资助金额:
    $ 41.25万
  • 项目类别:
Doctoral and Post-doctoral Training in Craniofacial Biology
颅面生物学博士和博士后培训
  • 批准号:
    9518803
  • 财政年份:
    2011
  • 资助金额:
    $ 41.25万
  • 项目类别:
DOCTORAL AND POST-DOCTORAL TRAINING IN CRANIOFACIAL BIOLOGY
颅面生物学博士和博士后培训
  • 批准号:
    8884400
  • 财政年份:
    2011
  • 资助金额:
    $ 41.25万
  • 项目类别:
DOCTORAL AND POST-DOCTORAL TRAINING IN CRANIOFACIAL BIOLOGY
颅面生物学博士和博士后培训
  • 批准号:
    8880175
  • 财政年份:
    2011
  • 资助金额:
    $ 41.25万
  • 项目类别:
DOCTORAL AND POST-DOCTORAL TRAINING IN CRANIOFACIAL BIOLOGY
颅面生物学博士和博士后培训
  • 批准号:
    8269856
  • 财政年份:
    2011
  • 资助金额:
    $ 41.25万
  • 项目类别:

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通过能量代谢转变调节成熟期成釉细胞的 RA-SA 循环
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Tooth formation using mouse tooth progenitor cells that can fluorescently label odontoblasts and ameloblasts.
使用可以荧光标记成牙本质细胞和成釉细胞的小鼠牙齿祖细胞形成牙齿。
  • 批准号:
    19K10041
  • 财政年份:
    2019
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    $ 41.25万
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Regulation of enamel matrix protein secretion in ameloblasts
成釉细胞釉质基质蛋白分泌的调节
  • 批准号:
    10192703
  • 财政年份:
    2017
  • 资助金额:
    $ 41.25万
  • 项目类别:
The role of non-muscle myosin II for the niche formation of dental epithelial stem cells and the movement of ameloblasts
非肌肉肌球蛋白II对牙上皮干细胞生态位形成和成釉细胞运动的作用
  • 批准号:
    16K11453
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    $ 41.25万
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    Grant-in-Aid for Scientific Research (C)
Relationship between blood vessels in stellate reticulum and microenvironmental change in ameloblasts differentiation
星状网血管与成釉细胞分化微环境变化的关系
  • 批准号:
    26462794
  • 财政年份:
    2014
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    $ 41.25万
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    Grant-in-Aid for Scientific Research (C)
Expression profiling of the protease Gm5771 in ameloblasts
成釉细胞中蛋白酶 Gm5771 的表达谱
  • 批准号:
    465872-2014
  • 财政年份:
    2014
  • 资助金额:
    $ 41.25万
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    University Undergraduate Student Research Awards
The novel exploration of the factors for maintaining the function of ameloblasts and stratum intermedium during mouse enamel formation
小鼠牙釉质形成过程中成釉细胞和中间层功能维持因素的新探索
  • 批准号:
    25861741
  • 财政年份:
    2013
  • 资助金额:
    $ 41.25万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Role of ameloblastin for ameloblasts and enamel formation
成釉细胞蛋白对成釉细胞和牙釉质形成的作用
  • 批准号:
    8354357
  • 财政年份:
    2012
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    $ 41.25万
  • 项目类别:
Role of ameloblastin for ameloblasts and enamel formation
成釉细胞蛋白对成釉细胞和牙釉质形成的作用
  • 批准号:
    8699033
  • 财政年份:
    2012
  • 资助金额:
    $ 41.25万
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