Core B

项目摘要-核心B 分子生物学核心的主要目标是为项目1提供专业和高效的实验室服务 (Saper)、项目2(Scammell)、项目3(Fuller)和项目4(Dymecki/Vanderhorst)。我们还预计， 我们的转录学工作将为项目5(Wellman)的选择性药理靶标提供信息。少校 核心B的职责是对突变小鼠群体进行管理和基因分型，进行原位杂交 (RNAScope)，准备病毒载体，包括开发新的基因结构，并执行 单细胞(scRNA-seq)和单核转录组(SNUC-seq)，包括协助生物信息学 转录组数据集的分析。通过提供这些集中服务，Core B将最大限度地提高 在本PPG中提出的几种更复杂的分子技术有成功的机会。我们的 这些方法的多年经验最大限度地增加了复杂的分子技术 在这份PPG中提出的建议将会成功。

PROJECT SUMMARY – Core B The major goals of the Molecular Biology Core is to provide expert and efficient laboratory services to Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Dymecki/Vanderhorst). We also anticipate that our transcriptomics work will inform selective pharmacological targets for Project 5 (Wellman). The major duties of Core B are management and genotyping of mutant mouse colonies, performing in situ hybridization (RNAscope), preparation of viral vectors, including the development of new genetic constructs, and performing single cell (scRNA-seq) and single nuclei transcriptomics (sNuc-seq), including assistance with bioinformatic analysis of the transcriptomics datasets. By providing these centralized services, Core B will maximize the chance of success with several of the more complicated molecular techniques proposed in this PPG. Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.