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Core B

核心B

基本信息

批准号：
10491083
负责人：
Patrick M Fuller
金额：
$ 19.99万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10491083
关键词：
Bioinformatics Brain region Breeding Budgets Cell Nucleus Cells Custom Data Set Development EGR2 gene Elements Engineering Enterobacteria phage P1 Cre recombinase FLP recombinase FOXP2 gene Funding Generations Genes Genetic Genetic Recombination Genotype Goals Histocytochemistry Histologic Hybrids In Situ Hybridization In Vitro Laboratories Maintenance Methods Microinjections Mission Molecular Molecular Biology Mus Mutant Strains Mice Neurons Pharmacology Phenotype Plasmids Preparation Production Protocols documentation Publications Recombinants Scheme Series Services Signal Transduction Sleep Apnea Syndromes Specificity Techniques Technology Tissues Toxic effect Toxin Transgenic Organisms Update Viral Viral Vector Work adeno-associated viral vector base cell type cost differential expression experience experimental study improved in vivo evaluation interest mRNA Expression neurochemistry promoter relating to nervous system single-cell RNA sequencing success tool transcriptomics

项目摘要

PROJECT SUMMARY – Core B The major goals of the Molecular Biology Core is to provide expert and efficient laboratory services to Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Dymecki/Vanderhorst). We also anticipate that our transcriptomics work will inform selective pharmacological targets for Project 5 (Wellman). The major duties of Core B are management and genotyping of mutant mouse colonies, performing in situ hybridization (RNAscope), preparation of viral vectors, including the development of new genetic constructs, and performing single cell (scRNA-seq) and single nuclei transcriptomics (sNuc-seq), including assistance with bioinformatic analysis of the transcriptomics datasets. By providing these centralized services, Core B will maximize the chance of success with several of the more complicated molecular techniques proposed in this PPG. Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.

项目概要-核心B 分子生物学核心的主要目标是为项目1提供专业和高效的实验室服务（Saper）、项目2（Scammell）、项目3（Fuller）和项目4（Dymecki/Vanderhorst）。我们还预计，我们的转录组学工作将为项目5（Wellman）提供选择性药理学靶点。主要核心B的职责是突变小鼠集落的管理和基因分型，进行原位杂交（RNAscope），病毒载体的制备，包括新的遗传构建体的开发，以及进行单细胞（scRNA-seq）和单核转录组学（sNuc-seq），包括生物信息学辅助转录组学数据集的分析。通过提供这些集中式服务，核心B将最大限度地成功的机会与几个更复杂的分子技术提出了这个PPG。我们多年的经验与这些方法最大的可能性，复杂的分子技术，在这个PPG中提出的建议将会成功。