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The Hemostasis, Thrombosis, and Inflammation Models Core

止血、血栓形成和炎症模型核心

基本信息

批准号：
10676889
负责人：
Wolfgang Bergmeier
金额：
$ 34.75万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-05 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10676889
关键词：
Adoptive Transfer Affect Animal Model Antibodies Area Biochemistry Biological Assay Biophysics Bleeding time procedure Blood Platelets Carotid Arteries Data Data Analyses Development Doctor of Philosophy Equipment Feedback Genotype Hemostatic function IACUC Imaging Techniques In Vitro Individual Inferior vena cava structure Inflammation Injury Laser injury Lung Maintenance Megakaryocytes Mesentery Methodology Modeling Monomeric GTP-Binding Proteins Mouse Strains Mus Names North Carolina Procedures Production Protocols documentation Quarantine Reaction Reproducibility Research Research Personnel Resources Saphenous Vein Seminal Services Skin Stenosis Study models Tail Technical Expertise Thrombosis Universities Venous arteriole cost ferric chloride in vivo intravital microscopy laboratory experiment mid-career faculty mouse model neutrophil programs

项目摘要

Abstract The Hemostasis, Thrombosis, and Inflammation Models Core (Core A) will provide the resources, equipment and technical expertise to conduct the in vivo assays that are a critical component of all projects included in this program. The main services provided by the core are: 1. Organize shipment of mice from UC San Diego to UNC Chapel Hill and to establish colonies for select strains at UNC Chapel Hill 2. Subject mice to various models of hemostasis, thrombosis, and inflammation 3. Analyze data and provide project investigators with results and feedback on how to move forward with projects/ mouse model studies. Including an animal models core will increase reproducibility in results obtained for the various projects in this PPG, and drastically reduce costs for labor and set-up/ maintenance of major equipment. Core A will be directed by Dr. Wolfgang Bergmeier, an Associate Professor of Biochemistry and Biophysics at the University of North Carolina who has many years of expertise in the use of animal models (Hemostasis, Thrombosis, and Inflammation) and state-of-the-art intravital microscopy (IVM) imaging techniques. In his own research, he has made seminal contributions to our understanding of how the small GTPase Rap1 and its regulators affect platelet and neutrophil functions – a major focus area of this program.

摘要止血、血栓和炎症模型核心(核心A)将提供资源、设备和技术专业知识来进行体内分析，这是本项目中所有项目的关键组成部分程序。核心提供的主要服务包括： 1.组织从加州大学圣地亚哥分校到北卡罗来纳大学教堂山的老鼠运输，并为选定的品系建立菌落在北卡罗来纳大学教堂山 2.使小鼠接受各种止血、血栓形成和炎症模型 3.分析数据并向项目调查人员提供如何推进的结果和反馈项目/鼠标模型研究。包括动物模型核心将增加在不同项目中获得的结果的重复性 PPG，并大幅降低劳动力成本和主要设备的安装/维护成本。核心A将被定向作者沃尔夫冈·伯格迈尔博士，诺斯大学生物化学和生物物理学副教授卡罗莱纳，在使用动物模型(止血、血栓和炎症)和最先进的活体显微镜(IVM)成像技术。在他自己的研究中，他有对我们理解小GTP酶Rap1及其调节因子如何影响血小板做出了开创性的贡献以及中性粒细胞功能--这是该计划的一个主要重点领域。