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Regulatory Pathways in Borrelial Pathogenesis

伯氏疏螺旋体发病机制的调节途径

基本信息

批准号：
10677726
负责人：
Jenny A. Hyde
金额：
$ 66.31万
依托单位：
TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-05 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10677726
关键词：
Address Adhesions Affect Arbovirus Infections Arthropod Vectors Attenuated Bacteria sigma factor KatF protein Binding Binding Sites Biochemical Borrelia Borrelia burgdorferi Borrelia oxidative stress regulator ChIP-seq Complement Complex Consensus Sequence Cysteine DNA DNA Binding DNA Sequence Data Diagnosis Etiology Event Exhibits Gene Expression Gene Expression Profile Gene Expression Regulation Genes Genetic Transcription Goals Homologous Gene Infection Infectious Agent Life Cycle Stages Link Lyme Disease Mammals Mediating Metals Methodology Molecular Chaperones Mus Nucleic Acid Binding Oxidation-Reduction Oxidative Stress Pathogenesis Pathogenicity Pathway interactions Persons Phenotype Post-Transcriptional Regulation Prevention Prevention strategy Production Promoter Regions Proteins Public Health Publications RNA RNA Binding Recording of previous events Regulation Regulator Genes Regulatory Pathway Regulon Research Role Scheme Structure Testing Therapeutic Time Transcript Transcriptional Regulation Translations Uncertainty United States Untranslated RNA Virulence Work arthropod-borne crosslinking and immunoprecipitation sequencing dimer experience experimental study genetic regulatory protein innovation insight interest metalloregulatory protein mutant novel skills stem tick transmission transcriptome transcriptome sequencing transmission process virulence gene

项目摘要

PROJECT SUMMARY The etiologic agent of Lyme Disease, Borrelia burgdorferi (Bb), is the leading arthropod-borne infection in the United States with over 470,000 cases diagnosed annually. Given that Lyme Disease is a significant public health concern, studies addressing how Bb establishes and maintains infection are vital to evaluating its pathogenic potential and developing prevention or therapeutic strategies. It is well known that Bb modifies its transcriptional profile throughout its pathogenic lifecycle, particularly during transmission from the arthropod vector into mammals. BosR is an important transcriptional regulator that functions as a global metalloregulatory protein and alters the expression of genes needed for mammalian colonization. Specifically, BosR is required for experimental infection in mice and is needed for the activation of the RpoS/RpoN/Rrp2 cascade that drives the expression of genetically unlinked genes, including virulence determinants required for Bb infection. While there is no doubt that the BosR-dependent regulation of rpoS is important to borrelial infectivity, there are additional genes that BosR regulates either directly or indirectly that may factor into Bb infection. How BosR is linked to this complex regulatory network has not been thoroughly addressed. Here, several novel BosR regulatory functions are described, heretofore unknown, including the recognition of RNA by BosR. The Preliminary Data shows that BosR binds to small non-coding RNA and exhibits chaperone activity suggesting that BosR forms a complex with sRNAs that binds to Bb transcripts and targets them for degradation or enhanced translation. This form of post-transcriptional regulation by BosR represents a new and innovative regulatory scheme that adds to its known DNA binding activity. In Aim 1, a subset of sRNAs that BosR binds will be evaluated, the transcripts recognized by the BosR::sRNA complex identified, and a link with the BosR-associated sRNAs to borrelial pathogenesis established. In Aim 2, recent ChIP-seq data will be mined to assess novel BosR DNA binding. Preliminary binding studies confirmed the ChIP-seq data for two targets and suggested that the redox status and metal coordination of BosR may alter DNA binding. BosR DNA binding will be analyzed to determine how the redox and metal binding status of BosR changes the target sequences recognized. Unique BosR binding motifs will be tracked, and a consensus sequence ascertained. Finally, experiments will be conducted to determine the phenotype of a bosR mutant that ectopically produces RpoS to query the direct role of BosR in Bb infection. Taken together, this proposal will provide new appreciation into how BosR-mediated transcriptional and post-transcriptional regulation ensues and novel insight into how BosR integrates the redox status of Bb to affect nucleic acid binding and borrelial pathogenesis.

项目总结