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Contribution of cell mechanics in promoting collective behavior for supracellular migration

细胞力学在促进超细胞迁移集体行为中的贡献

基本信息

批准号：
10680022
负责人：
Selena Gupta
金额：
$ 4.77万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10680022
关键词：
Actomyosin Affect Back Behavior Bilateral Biochemical Biological Phenomena Biological Process Cell Shape Cells Cellular Morphology Characteristics Chordata Communication Computer Models Coupling Cytoskeletal Modeling Cytoskeleton Development Dorsal Embryo Endoderm Epidermis Equilibrium Exhibits Generations Hydrostatic Pressure Individual Intercellular Junctions Invertebrates Maintenance Measurement Mechanics Methods Modeling Morphogenesis Morphology Movement Myosin ATPase Neoplasm Metastasis Relaxation Research Proposals Reverse engineering Role Shapes Side Speed Structure System Tail Testing Tissues Travel Urochordata Vertebrates Work biophysical properties cell motility embryo tissue improved in vivo in vivo Model in vivo imaging kinematics mechanical signal migration optogenetics progenitor spatiotemporal tool transmission process wound healing

项目摘要

Project Summary Highly ordered, directed movement in collective cell migration is a result of the magnitude of coordination and communication that takes place at both the cellular and tissue level. In this proposal, we focus on understanding the mechanisms that contribute to the system behaving as a ‘collective’ to achieve such phenomenon. The cardiopharyngeal progenitors of the tunicate Ciona provide the simplest model of collective cell migration, with cohesive bilateral cell pairs polarizing and migrating between the ventral epidermis and trunk endoderm. Bernadskaya & Yue et al. 2021 found that the collective cell migration of Ciona exhibits supracellular characteristics; while each cell has the machinery to migrate individually, for successful development, the cells travel together and take on the identity of either the ‘leader’ or ‘trailer’. How this collective polarity is established and communicated between the two cells, and the type of information being exchanged, remains to be elucidated. We propose that this collective migration is driven by a “rear-engine” with supracellular polarity, as mechanical information flows from the trailer cell to the leader cell. We speculate that the cell:cell junction serves as a point of plasticity; preliminary work has shown that the junction has relatively low levels of actomyosin, allowing it to be under low tension and easily deformable. We hypothesize the two cells are utilizing cell shape – specifically the direction and degree of deformation at the cell:cell junction – and hydrostatic pressure as a method for mechanical coupling. To test our hypothesis, we will use in vivo images to reverse-engineer forces, extract force measurements, and build computational models of migration. Then, we will experimentally validate the role of the cell:cell junction as an information hub contributing to supracellular polarity, cytoskeletal organization, as well as force generation and transmission.

项目总结