The role of Vpr-mediated cell cycle dysregulation in HIV-associated kidney disease

Vpr 介导的细胞周期失调在 HIV 相关肾病中的作用

• 批准号: 10678878
• 负责人: John Cijiang He
• 金额: $ 74.03万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10678878
• 关键词: AIDS-Associated Nephropathy; Acceleration; Animal Model; Anti-Retroviral Agents; Antimitotic Agents; Apoptosis; Apoptotic; Atrophic; Attenuated; Biopsy Specimen; Bypass; CDC2 gene; CDT1 Gene; Cell Cycle; Cell Cycle Arrest; Cell Cycle Deregulation; Cell Death; Cells; Cessation of life; Chromatin; Chronic; Chronic Kidney Failure; Clinical; Complement; Cytokinesis; DNA Replication Factor; DNA biosynthesis; Defect; Development; Diabetes Mellitus; Diagnosis; Disease Progression; End stage renal failure; Epithelial Cells; Equilibrium; Fibrosis; G2/M Arrest; Gene Expression; Genes; HIV; HIV Infections; HIV therapy; HIV-1; Human; Hyperplasia; Hypertrophy; In Situ; In Vitro; Incidence; Individual; Inflammation; Injury; Injury to Kidney; Intervention; Kidney; Kidney Diseases; Knowledge; Licensing; Licensing Factor; Measures; Mediating; Mitosis; Mitotic; Mitotic Cell Cycle; Modeling; Molecular; Monitor; Mus; PLK1 gene; Pathogenesis; Pathologic; Patients; Phenotype; Phosphotransferases; Polyploid Cells; Polyploidy; Prevalence; Proteins; Proteinuria; RNA; Reporter; Reporting; Risk; Role; Study models; TP53 gene; Therapeutic Effect; Therapeutic Intervention; Time; Transgenic Mice; Tubular formation; Viral Load result; Viral Proteins; age related; antiretroviral therapy; cancer cell; cell injury; comorbidity; comparative; effective therapy; epidemiological model; in vivo; inhibitor; interstitial; kidney biopsy; kidney fibrosis; mouse model; new therapeutic target; novel; pharmacologic; premature; single-cell RNA sequencing; transcriptome sequencing; transcriptomic profiling; transcriptomics; vpr Gene Products

PROJECT SUMMARY With the widespread use of combination antiretroviral agents, the incidence of HIV-associated nephropathy (HIVAN) has dramatically decreased in the recent years. Yet, the prevalence of chronic kidney disease (CKD) and end-stage kidney disease in patients living with HIV remains high, suggesting that HIV predisposes patients to increased risk for chronic kidney disease. Indeed, several lines of evidence from recent epidemiological and animal model studies indicate that concurrent HIV infection and age-related comorbidities, such as diabetes mellitus, have a synergistic effect on the incidence of chronic kidney disease, thereby necessitating an examination of mechanisms by which HIV infection even at low viral load accelerates the progression of CKD. Among the HIV-1 viral proteins, we previously showed that HIV viral protein R (Vpr) can induce cell cycle dysregulation, apoptosis, and polyploidy in renal tubular cells. However, the importance and consequences of Vpr-mediated cell cycle arrest and polyploidy has not been fully explored in the setting of kidney disease. In this proposal, we will further dissect the mechanisms dictating the cell fates of Vpr- expressing renal tubular using in vitro approaches (Aim 1). Similarly, using transgenic mice expressing Vpr in renal tubular epithelial cells, we will characterize the cell cycle dysregulation and gene expression at single-cell levels, and determine whether the pharmacological intervention of cell cycle dysregulation can attenuate kidney disease progression in this model, as well as in HIVAN mouse model, Tg26 (Aim 2). To complement the findings in Aims 1 and 2, we will assess the expression of genes and cell cycle regulators in kidney biopsy samples of HIV+ CKD patients (Aim 3). We will also perform transcriptomic profiling for comparative analyses with findings in murine kidneys. Our results will provide a better understanding of the underlying molecular mechanisms by which chronic HIV infection accelerates the progression of CKD and a proof-of-concept for novel target treatment for CKD in HIV patients.

项目概要 随着联合抗逆转录病毒药物的广泛使用，HIV相关肾病的发病率 （HIVAN）近年来急剧下降。然而，慢性肾脏病（CKD）的患病率 HIV 感染者的终末期肾病发病率仍然很高，这表明 HIV 易导致 患者患慢性肾脏病的风险增加。事实上，最近的几条证据 流行病学和动物模型研究表明，并发艾滋病毒感染和与年龄相关的合并症， 糖尿病等疾病，对慢性肾脏病的发病率有协同作用，从而 有必要研究艾滋病毒感染即使在低病毒载量下也会加速感染的机制。 CKD 的进展。在HIV-1病毒蛋白中，我们之前表明HIV病毒蛋白R（Vpr）可以 诱导肾小管细胞的细胞周期失调、细胞凋亡和多倍体。然而，重要性和 Vpr 介导的细胞周期停滞和多倍体的后果尚未在以下情况中得到充分探索： 肾脏疾病。在本提案中，我们将进一步剖析决定 Vpr-细胞命运的机制 使用体外方法表达肾小管（目标 1）。同样，使用表达 Vpr 的转基因小鼠 肾小管上皮细胞，我们将描述单细胞的细胞周期失调和基因表达 水平，并确定细胞周期失调的药物干预是否可以减弱 该模型以及 HIVAN 小鼠模型 Tg26 中的肾脏疾病进展（目标 2）。为了补充 根据目标 1 和 2 中的发现，我们将评估肾活检中基因和细胞周期调节因子的表达 HIV+ CKD 患者样本（目标 3）。我们还将进行转录组分析以进行比较分析 在小鼠肾脏中的发现。我们的结果将有助于更好地理解潜在的分子 慢性 HIV 感染加速 CKD 进展的机制以及概念验证 HIV 患者 CKD 的新型靶向治疗。

项目成果

HIV-1 infection of renal epithelial cells: 30 years of evidence from transgenic animal models, human studies and in vitro experiments.

• DOI: 10.1186/s12977-023-00617-8
• 发表时间: 2023-03-16
• 期刊: Retrovirology
• 影响因子: 3.3