Novel methods for large-scale genomic interval comparison

大规模基因组区间比较的新方法

• 批准号: 10678947
• 负责人: Nathan Sheffield
• 金额: $ 38.4万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-10 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10678947
• 关键词: ATAC-seq; Address; Affect; Algorithms; Area; Automobile Driving; Binding; Biological; Biomedical Research; ChIP-seq; Chromosomes; Communities; DNA; Data; Data Set; Data Sources; Databases; Dimensions; Disease; Disease Outcome; Epigenetic Process; Gene Expression; Gene Expression Regulation; Genes; Genetic; Genetic Annotation; Genetic Variation; Genome; Genomic Segment; Genomics; Goals; Health; Human; Human Genome; Individual; Investments; Learning; Machine Learning; Measures; Methods; Natural Language Processing; Outcome; Protocols documentation; Resources; Signal Transduction; Source; Speed; Structure; Techniques; Text; Work; design; epigenome; epigenomics; experimental study; genetic analysis; genome analysis; human reference genome; improved; innovation; machine learning method; markov model; novel; novel strategies; tool; trait; vector

ABSTRACT Epigenome data are driving discovery in biomedical analysis of genetic variation and gene regulation. Epigenome data produced by experimental protocols such as ATAC-seq or ChIP-seq are often summarized into sets of genomic intervals defined by a chromosome plus start and end coordinates. Databases now provide hundreds of thousands of such region sets, each containing potentially hundreds of thousands of individual regions. This data holds tremendous promise to understand gene regulation and disease because many health outcomes are affected by genetic variation or epigenetic perturbation in regulatory DNA. Many different tools and methods have been developed to assess such sets of genomic intervals. These ap- proaches are used for a broad array of biomedical research, such as annotating genetic variation associated with disease traits. Supporting region-based analyses, we and others have developed novel data structures and algorithms to compare similarity of region sets and to compute overlaps between interval sets, enabling interval comparisons on millions of regions. But as the genomic interval set data sources grow in size and scope, we require both faster algorithms and novel methods to compare this important data type. As the amount of available data increases, it is becoming intractable to compute exact overlaps. Furthermore, the fastest algorithms only analyze pure intervals, not signal values, which could be used to compare interval sets more accurately. Existing approaches have made little progress in the area of defining canonical interval sets to simplify analysis even further. Here, we address these limitations in several ways: First, we develop novel, more scalable algorithms using approximate computations and define the idea of interval set universes to consolidate analysis. Second, we develop an innovative approach to analyzing region sets that goes beyond simply counting overlaps, instead relying on cutting-edge machine learning methods to learn and measure similarity more accurately. We propose a novel set theoretic approach building on techniques from natural language processing to compare intervals. Together, we propose a first-pass filter that can be reasonably computed on data sets containing billions to trillions of genomic intervals, followed by a more accurate analysis to identify more subtle relationships among region sets. These advances will improve both the efficiency and accuracy of existing biomedical research approaches, and open the door to new ways of exploring the vast and growing corpus of genome interval data.

摘要