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Development of Nav1.1 Enhancers to Treat Alzheimer's Disease

开发 Nav1.1 增强剂来治疗阿尔茨海默病

基本信息

批准号：
10701877
负责人：
Michael Pleiss
金额：
$ 124.95万
依托单位：
CURE NETWORK DOLBY ACCELERATION PARTNERS LLC
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-15 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10701877
关键词：
APP-PS1 Acceleration Acute Alzheimer&apos s Disease Alzheimer&apos s disease model Alzheimer&apos s disease pathology Alzheimer&apos s disease patient Amyloid Animal Model Astrocytes Bacterial Artificial Chromosomes Behavioral Biological Assay Biological Availability Brain Cardiac Cell Line Cells Central Nervous System Diseases Chemicals Clinical Trials Cognitive Deposition Development Disease Dose Drug Design Drug Kinetics Electrocardiogram Electroencephalography Enhancers Event Functional disorder Future Genetic Grant Heart Human Hyperactivity Immobilization Impaired cognition In Vitro Inflammation Interneurons Intervention J20 mouse Link Liver Microsomes Magnetism Mediator Microglia Molecular Molecular Target Monitor Mus Nerve Degeneration Neurons Oral Administration Pathogenicity Pathologic Pathology Patients Pharmaceutical Chemistry Pharmaceutical Preparations Pharmacologic Substance Phase Physiological Pre-Clinical Model Property Reporting Role SCN1A protein SCN2A protein Sensory Series Slice Small Business Innovation Research Grant Sodium Channel Specificity System Toxic effect Transplantation Wild Type Mouse cognitive function design drug candidate drug development efficacy testing genome-wide heart function human disease improved in vivo in vivo evaluation intraperitoneal meter mild cognitive impairment mortality mouse model network dysfunction neuropathology neurovascular novel novel drug class novel therapeutics optogenetics overexpression patch clamp phase 1 study phase 2 study pre-clinical prevent programs restoration screening side effect small molecule small molecule therapeutics stability testing tau Proteins tau aggregation therapeutic target transcriptome transcriptomics voltage

项目摘要

ABSTRACT CNDAP is developing a new class of drugs, acting through a unique mechanism of action, to reverse early pathophysiological and cognitive alterations in Alzheimer’s disease (AD). Recent studies indicate that network dysfunction, which includes network hyperactivity, altered oscillatory rhythms, and hyper- synchronized networks, is an early pathogenic event found in preclinical models of AD and in patients with early stages of AD. Network dysfunction contributes to cognitive abnormalities, Ab and tau accumulation, and neurodegeneration. In animal models, network dysfunction can be restored by enhancing inhibitory (GABAergic) interneuron-dependent gamma rhythms via optogenetic/sensory stimulation or genetic overexpression of Nav1.1. This restoration of gamma rhythms in the AD models leads to reduced amyloid and tau deposition, neurodegeneration, microglia and astrocytic activation, inflammation, neurovascular alterations, AD-induced genome-wide transcriptomics changes, altered oscillatory activity, and cognitive decline. Because overexpression of the sodium channel Nav1.1 by as little as 25% restores gamma rhythms to normal levels in AD models, we are developing small molecule therapeutics designed to safely increase Nav1.1 activity in the brain to treat AD. We have identified several small molecule chemotypes that effectively enhance human Nav1.1 currents in cell lines and interneuron-dependent gamma oscillations in brain slices. Systemic intraperitoneal administration of high doses of a Nav1.1 enhancer in vivo in mice produced no overt toxicity or behavioural side effects but significantly increased endogenous gamma oscillatory activity in wildtype mice, suggesting that our compounds are brain penetrant and may have beneficial effects following systemic administration. In this fast-track SBIR grant, we propose to further develop Nav1.1 enhancers to treat AD. In Phase 1 studies, we will employ medicinal chemistry and SAR analysis to identify structurally unique, potent and selective Nav1.1 enhancers to expand our chemical composition of matter. Pharmacokinetics and brain bioavailability analyses will be used to select the most active compounds with optimal pharmaceutical properties. Our milestone to achieve to move to Phase 2 studies is the identification of at least one structurally novel Nav1.1 enhancer that selectively and significantly increases gamma oscillations ex vivo in brain. Phase 2 studies are designed to establish the ex vivo and in vivo efficacy of our Nav1.1 enhancers to restore network dysfunction in preclinical AD mouse models by their ability to prevent network hypersynchrony and abnormal oscillatory activity, restore genome-wide transcriptomic changes and to reduce cognitive impairment, neuropathology and improve survival to support their future development to treat AD patients.

摘要