P30-Core Grant for Vision Research Core A

摘要--履行机构核心A 结构生物学和成像(SBI)核心加强了AVRC的视觉研究 通过帮助成员确定正常和 眼病、疾病模型及实验后的组织结构异常 治疗。该核心为AVRC成员提供设施和服务，以 通过最新技术确定结构变化及其与功能的关系 组织学、电子显微镜、共聚焦扫描激光显微镜和实验 动物外科成像。履行机构核心还支持获取结构数据和 为AVRC成员的新倡议解释这一数据，重点是 视网膜功能、视网膜变性/损伤、RPE形态测量、眼肿瘤学和 基因/药物传递。SBI核心使参与控制的蛋白质本地化 健康和疾病中的视网膜功能.视网膜和视神经组织变化的成像 神经退行性变；RPE形态计量学改变与基因和 原发和转移性眼部肿瘤的成像技术的发展， 包括与MRI造影剂和远程成像的组织相关性；以及研究 眼组织改变与基因和药物给药至眼后部有关 车厢。这一核心设施可捕获和移植高分辨率大图像 从设备到其他核心模块。此核心帮助非熟练或新手NEI R01 获奖者了解和使用大学的超分辨率显微镜核心 接近了。此核心支持图像捕获和处理，通常 超越了在正常R01机制下可能提出的功能。

Summary SBI Core A The Structural Biology and Imaging (SBI) Core enhances vision research of AVRC members by assisting them in determining the relationship between normal and abnormal tissue structure in eye diseases, disease models and after experimental treatments. This SBI Core provides facilities and services for AVRC members to determine structural changes and how they relate to function through state of the art histology, electron microscopy, confocal scanning laser microscopy and experimental animal surgery imaging. The SBI Core also supports obtaining structural data and interpretation of this data for new initiatives of AVRC members with an emphasis on retinal function, retinal degeneration/injury, RPE morphometry, ocular oncology, and gene/drug delivery. The SBI Core enables localization of proteins involved with control of retinal function in health and disease; imaging of tissue changes in retinal and optic nerve degenerations; correlation of RPE morphometric changes with genotype and function; development of imaging techniques for primary and metastatic ocular tumors, including tissue correlations with MRI contrast agents and tele-imaging; and studying ocular tissue changes relative to gene and drug delivery to the posterior ocular compartment. This core facilities capture and porting of high resolution large images from devices to other Core modules. This Core helps unskilled or neophyte NEI R01 awardees to understand and use university cores for super-resolution microscopy approaches. This Core supports image capture and processing that would normally be beyond the capabilities that could be proposed under a normal R01 mechanism.