P30-Core Grant for Vision Research Core A

SBI Core A摘要 结构生物学和成像（SBI）核心增强了AVRC的视觉研究 协助他们确定正常和 眼部疾病、疾病模型和实验后的异常组织结构 治疗。该SBI核心为AVRC成员提供设施和服务， 通过最新技术水平确定结构变化及其与功能的关系 组织学、电子显微镜、共聚焦扫描激光显微镜和实验 动物外科成像SBI Core还支持获取结构数据， 为AVRC成员的新倡议解释这些数据，重点是 视网膜功能、视网膜变性/损伤、RPE形态测定、眼肿瘤学，以及 基因/药物递送。SBI核心能够定位参与控制蛋白质， 健康和疾病时的视网膜功能;视网膜和视神经组织变化的成像 神经变性; RPE形态学变化与基因型和 功能;原发性和转移性眼部肿瘤成像技术的发展， 包括与MRI造影剂和远程成像的组织相关性;和研究 与基因和药物递送至眼后部相关的眼组织变化 车厢这一核心设施捕获和移植高分辨率的大图像 从设备到其他核心模块。此核心帮助不熟练或新手NEI R 01 获奖者了解和使用大学核心的超分辨率显微镜 接近。此核心支持通常情况下 超出了在正常R 01机制下可以提出的能力。

Summary SBI Core A The Structural Biology and Imaging (SBI) Core enhances vision research of AVRC members by assisting them in determining the relationship between normal and abnormal tissue structure in eye diseases, disease models and after experimental treatments. This SBI Core provides facilities and services for AVRC members to determine structural changes and how they relate to function through state of the art histology, electron microscopy, confocal scanning laser microscopy and experimental animal surgery imaging. The SBI Core also supports obtaining structural data and interpretation of this data for new initiatives of AVRC members with an emphasis on retinal function, retinal degeneration/injury, RPE morphometry, ocular oncology, and gene/drug delivery. The SBI Core enables localization of proteins involved with control of retinal function in health and disease; imaging of tissue changes in retinal and optic nerve degenerations; correlation of RPE morphometric changes with genotype and function; development of imaging techniques for primary and metastatic ocular tumors, including tissue correlations with MRI contrast agents and tele-imaging; and studying ocular tissue changes relative to gene and drug delivery to the posterior ocular compartment. This core facilities capture and porting of high resolution large images from devices to other Core modules. This Core helps unskilled or neophyte NEI R01 awardees to understand and use university cores for super-resolution microscopy approaches. This Core supports image capture and processing that would normally be beyond the capabilities that could be proposed under a normal R01 mechanism.