Cellular Imaging Core (CIC)

摘要 细胞成像核心（CIC）在IDDRC的多学科研究管道中发挥着关键作用 波士顿儿童医院和哈佛医学院的研究人员。位于Kirby Neurobiology 波士顿儿童医院的中心，这个共享的显微镜设施为研究人员提供了高端和 专业的显微镜和数字成像方法，他们可以利用这些方法来了解 与神经发育障碍相关的机制和结构变化。核心提供宽场， 共焦（多光子、激光扫描、旋转盘）和超分辨率（受激发射损耗， STED）显微镜，用于感兴趣蛋白质的高分辨率亚细胞定位，用于跟踪活细胞， 延长的时间段，并用于在清醒的行为动物体内监测细胞群。这些 仪器伴随着对图像分析工作站的访问，重要的是， 一种教育计划，一对一地培训研究人员了解每种仪器的能力和局限性 使用的软件包。核心工作人员还可以与IDDRC研究人员密切合作， 设计、优化和实施定制设计的实验或分析方法的项目。此外，本发明还 核心提供仪器和技术课程，以告知科学界新的东西 为国家的最先进的显微镜，提供互动，动手讲习班的图像采集，分析和 处理并解决这些成像方法的鲁棒性问题。通过这些互动， 研究人员，核心识别并获得与我们的研究人员相关的新设备和技术。 总的来说，CIC在这里提出了一个与IDDRC调查人员合作的全面计划 和其他IDDRC核心，以降低实验室将新的和变革性的 显微镜技术，提高和加速我们对正常和病理性神经 发育、可塑性和功能。

ABSTRACT The Cellular Imaging Core (CIC) plays a pivotal role in the multidisciplinary research pipeline of IDDRC investigators at Boston Children’s Hospital and Harvard Medical School. Located within the Kirby Neurobiology Center of Boston Children’s Hospital, this shared microscopy facility offers researchers access to high-end and specialized microscopy and digital imaging approaches which they can harness to understand the underlying mechanisms and structural changes associated with neurodevelopmental disorders. The Core offers widefield, confocal (multiphoton, laser-scanning, spinning disk) and super-resolution (stimulated emission depletion, STED) microscopes for high resolution subcellular localization of proteins of interest, for tracking live cells over prolonged periods of time, and for monitoring populations of cells in vivo in an awake, behaving animal. These instruments are accompanied with access to image analysis workstations, and importantly, to an extensive educational program that trains researchers one-on-one on the capabilities and limitations of each instrument and software package used. Core staff can also collaborate closely with IDDRC researchers in core-assisted projects to design, optimize and implement custom designed experiments or analysis approaches. Additionally, the Core offers Instrumentation and Technology Courses to inform the scientific community about what is new for state-of-the-art microscopy, to provide interactive, hands-on workshops on image acquisition, analysis and processing and to address issues in robustness with these imaging methods. Through these interactions with researchers, the Core identifies and obtains new equipment and technology that is relevant to our researchers. Taken together, here the CIC proposes a comprehensive program of collaboration with IDDRC investigators and other IDDRC Cores to lower the barrier for laboratories to incorporate novel and transformative microscopy technologies that elevate and accelerate our understanding of normal and pathological neural development, plasticity and function.