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Cellular Imaging Core (CIC)

细胞成像核心 (CIC)

基本信息

批准号：
10681500
负责人：
Hisashi Umemori
金额：
$ 141.6万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-22 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10681500
关键词：
Acceleration Address Advisory Committees Animals Array tomography Attention Boston Candidate Disease Gene Cells Collaborations Communities Complex Computer software Consultations Custom Data Data Analyses Development Disease Education Educational workshop Equipment Experimental Designs Fund Raising Funding Funding Agency Future Goals Grant Image Image Analysis Imaging Techniques Imaging technology In Vitro Individual Institution Intellectual and Developmental Disabilities Research Centers Interdisciplinary Study Knowledge Laboratories Lasers Maintenance Microscope Microscopy Monitor Neurobiology Neurodevelopmental Disorder Neurologic Neurology Neurons Organ Pathologic Pediatric Hospitals Play Population Privatization Procedures Proteins Quality Control Reliability of Results Reproducibility of Results Research Research Personnel Resolution Resource Allocation Resources Role Sampling Scanning Services Signal Transduction Site Statistical Data Interpretation System Techniques Technology Time Training Training Programs United States National Institutes of Health Vendor Visualization Work analysis pipeline animal imaging awake cellular imaging data acquisition design digital imaging experimental study imaging approach imaging modality in vivo in vivo imaging instrument instrumentation interest medical schools member microscopic imaging model organism multi-photon multiphoton imaging neurodevelopment new technology novel optogenetics programs quantitative imaging superresolution microscopy tissue fixing ultra high resolution

项目摘要

ABSTRACT The Cellular Imaging Core (CIC) plays a pivotal role in the multidisciplinary research pipeline of IDDRC investigators at Boston Children’s Hospital and Harvard Medical School. Located within the Kirby Neurobiology Center of Boston Children’s Hospital, this shared microscopy facility offers researchers access to high-end and specialized microscopy and digital imaging approaches which they can harness to understand the underlying mechanisms and structural changes associated with neurodevelopmental disorders. The Core offers widefield, confocal (multiphoton, laser-scanning, spinning disk) and super-resolution (stimulated emission depletion, STED) microscopes for high resolution subcellular localization of proteins of interest, for tracking live cells over prolonged periods of time, and for monitoring populations of cells in vivo in an awake, behaving animal. These instruments are accompanied with access to image analysis workstations, and importantly, to an extensive educational program that trains researchers one-on-one on the capabilities and limitations of each instrument and software package used. Core staff can also collaborate closely with IDDRC researchers in core-assisted projects to design, optimize and implement custom designed experiments or analysis approaches. Additionally, the Core offers Instrumentation and Technology Courses to inform the scientific community about what is new for state-of-the-art microscopy, to provide interactive, hands-on workshops on image acquisition, analysis and processing and to address issues in robustness with these imaging methods. Through these interactions with researchers, the Core identifies and obtains new equipment and technology that is relevant to our researchers. Taken together, here the CIC proposes a comprehensive program of collaboration with IDDRC investigators and other IDDRC Cores to lower the barrier for laboratories to incorporate novel and transformative microscopy technologies that elevate and accelerate our understanding of normal and pathological neural development, plasticity and function.

抽象的细胞成像核心 (CIC) 在 IDDRC 的多学科研究管道中发挥着关键作用波士顿儿童医院和哈佛医学院的研究人员。位于柯比神经生物学内这个共享显微镜设施位于波士顿儿童医院中心，为研究人员提供了接触高端和他们可以利用专门的显微镜和数字成像方法来了解底层与神经发育障碍相关的机制和结构变化。核心提供宽视场、共焦（多光子、激光扫描、旋转盘）和超分辨率（受激发射损耗、 STED）显微镜，用于感兴趣蛋白质的高分辨率亚细胞定位，用于跟踪活细胞长时间，并用于监测清醒、行为动物体内的细胞群。这些仪器可以访问图像分析工作站，重要的是，可以访问广泛的教育计划，对研究人员进行一对一培训，了解每种仪器的功能和局限性以及使用的软件包。核心人员还可以与 IDDRC 研究人员在核心辅助方面密切合作设计、优化和实施定制设计的实验或分析方法的项目。此外，核心提供仪器和技术课程，向科学界通报最新动态对于最先进的显微镜，提供有关图像采集、分析和处理并解决这些成像方法的鲁棒性问题。通过这些互动研究人员，核心识别并获得与我们的研究人员相关的新设备和技术。综上所述，CIC 在此提出了与 IDDRC 调查人员合作的全面计划和其他 IDDRC 核心，以降低实验室纳入新颖和变革性的障碍显微镜技术可以提高和加速我们对正常和病理神经的理解发育、可塑性和功能。