Cellular Imaging Core (CIC)

细胞成像核心 (CIC)

• 批准号: 10239467
• 负责人: Hisashi Umemori
• 金额: $ 16.11万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-22 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10239467
• 关键词: Address; Advisory Committees; Animal Model; Animals; Array tomography; Attention; Boston; Candidate Disease Gene; Cells; Collaborations; Communities; Complex; Computer software; Consultations; Custom; Data; Data Analyses; Development; Disease; Educational workshop; Equipment; Experimental Designs; Fund Raising; Funding; Funding Agency; Future; Gene Proteins; Goals; Grant; Image; Image Analysis; Imaging Techniques; Imaging technology; In Vitro; Individual; Intellectual and Developmental Disabilities Research Centers; Interdisciplinary Study; Knowledge; Laboratories; Lasers; Maintenance; Microscope; Microscopy; Monitor; Neurobiology; Neurodevelopmental Disorder; Neurologic; Neurology; Neurons; Organ; Pathologic; Pediatric Hospitals; Play; Population; Privatization; Procedures; Proteins; Quality Control; Reproducibility of Results; Research; Research Personnel; Resolution; Resource Allocation; Resources; Role; Sampling; Scanning; Services; Signal Transduction; Site; Statistical Data Interpretation; Structure; System; Techniques; Technology; Time; Tissues; Training; Training Programs; United States National Institutes of Health; Vendor; Visualization; Work; analysis pipeline; animal imaging; awake; base; cellular imaging; data acquisition; design; digital imaging; experimental study; imaging approach; imaging modality; in vivo; in vivo imaging; instrument; instrumentation; interest; medical schools; member; microscopic imaging; multiphoton imaging; neurodevelopment; new technology; novel; optogenetics; programs; quantitative imaging

ABSTRACT The Cellular Imaging Core (CIC) plays a pivotal role in the multidisciplinary research pipeline of IDDRC investigators at Boston Children’s Hospital and Harvard Medical School. Located within the Kirby Neurobiology Center of Boston Children’s Hospital, this shared microscopy facility offers researchers access to high-end and specialized microscopy and digital imaging approaches which they can harness to understand the underlying mechanisms and structural changes associated with neurodevelopmental disorders. The Core offers widefield, confocal (multiphoton, laser-scanning, spinning disk) and super-resolution (stimulated emission depletion, STED) microscopes for high resolution subcellular localization of proteins of interest, for tracking live cells over prolonged periods of time, and for monitoring populations of cells in vivo in an awake, behaving animal. These instruments are accompanied with access to image analysis workstations, and importantly, to an extensive educational program that trains researchers one-on-one on the capabilities and limitations of each instrument and software package used. Core staff can also collaborate closely with IDDRC researchers in core-assisted projects to design, optimize and implement custom designed experiments or analysis approaches. Additionally, the Core offers Instrumentation and Technology Courses to inform the scientific community about what is new for state-of-the-art microscopy, to provide interactive, hands-on workshops on image acquisition, analysis and processing and to address issues in robustness with these imaging methods. Through these interactions with researchers, the Core identifies and obtains new equipment and technology that is relevant to our researchers. Taken together, here the CIC proposes a comprehensive program of collaboration with IDDRC investigators and other IDDRC Cores to lower the barrier for laboratories to incorporate novel and transformative microscopy technologies that elevate and accelerate our understanding of normal and pathological neural development, plasticity and function.

摘要 细胞成像核心(CIC)在IDDRC的多学科研究管道中起着举足轻重的作用 波士顿儿童医院和哈佛医学院的研究人员。位于Kirby神经生物学内 波士顿儿童医院中心，这一共享的显微镜设施为研究人员提供了高端和 专门的显微镜和数字成像方法，他们可以利用这些方法来了解潜在的 与神经发育障碍相关的机制和结构变化。酷睿提供宽阔的视野， 共焦(多光子、激光扫描、旋转盘)和超分辨率(受激发射耗尽， STED)显微镜，用于目标蛋白质的高分辨率亚细胞定位，用于跟踪活细胞 用于监测清醒的、行为正常的动物体内细胞数量。这些 仪器还可以访问图像分析工作站，更重要的是，可以访问广泛的 教育计划，对研究人员进行一对一培训，了解每种仪器的能力和局限性 和所用的软件包。核心工作人员还可以在核心协助下与国际发展研究中心的研究人员密切合作 设计、优化和实施定制设计的实验或分析方法的项目。另外， CORE提供仪器和技术课程，让科学界了解最新情况 对于最先进的显微镜，提供关于图像采集、分析和分析的交互式动手研讨会 并利用这些成像方法解决稳健性问题。通过这些与 对于研究人员，核心确定并获得与我们的研究人员相关的新设备和技术。 总而言之，CIC在这里提出了一个与IDDRC调查人员合作的全面计划 和其他IDDRC核心，以降低实验室纳入新的和变革性的门槛 提高和加速我们对正常和病理神经的理解的显微技术 发展、可塑性和功能。