Ocular Phenotyping Core

UAB视觉研究中心-眼表型核心 项目/摘要 眼结构和视觉功能的评估是视觉基础和转化研究的基础 无论是动物模型还是人体实验。广泛使用的技术包括光学相干 用于显示后段和前段中分层组织的断层摄影术（OCT），视网膜电图（ERG， 对于可分离成多个识别成分的聚集的视网膜信号），用于前视网膜的生物显微镜的裂隙灯 和后段，通过多种可视化模式（彩色，自发荧光，染料， 基于血管造影术、红外反射率）和视动性眼球震颤（以评估视觉控制、视敏度 和对比敏感度）。为了满足日益增长的UAB视力研究人员的需求，“眼表型核心” 建立了一套全面的仪器，并提供必要的支持 用于准确的眼部表型。具体仪器包括Bioptigen 840 nm SD-OCT和Micron IV数字 小动物眼底照相机，小动物包括大鼠、小鼠和 斑马鱼和Spectralis SDOCT用于大型动物和人类供体眼睛。一个S10补助金授予保罗博士 Gamlin在2020年允许购买三种新工具，这将大大提高两者的眼部表型 1）Zeiss Lumera 700 ReScan，带Resight 700手术显微镜， 术中光学相干断层扫描（OCT）成像; 2）FLEX模块Spectralis OCT 2系统， 在不同身体状态的动物中，使用结构和血管造影OCT对视网膜和视神经乳头进行成像， 3）眼前节CASIA SS-1000扫频源OCT（Tomey Corp.）用于对角膜，虹膜， 和透镜，以及小动物的整个眼睛。 该核心将支持15名UAB视觉科学家，其中包括13名计划中度到广泛的视觉科学家，他们都是 目前由NEI R 01资助。这一拟议核心小组的主任和副主任拥有大量出版物， 分别具有视网膜电图和OCT验证/解释的经验。核心的新方向 将包括完成一个新的基于LED的ERG系统，将简化这一测试。此外， 现有的AOSLO系统最初是为人类视网膜成像设计的，将重新设计，以便成像 啮齿动物和树鼩的眼睛。与此同时，荧光成像和细胞靶向 将添加光刺激。此外，将宣传眼部表型鉴定机会，以确定新的 通过对非眼部产生的小鼠模型进行全眼部表型筛选， 科学家们的动物最初是为了回答与他们的器官系统有关的问题， 兴趣

UAB Center Core for Vision Research - Ocular Phenotyping Core Project/Summary Abstract Assessment of ocular structures and visual function is essential to both basic and translational research in vision science whether in animal models or human subjects. Widely used technologies include optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, electroretinography (ERG, for massed retinal signals separable into multiple identified components), slit lamp for bio-microscopy of anterior and posterior segment, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye- based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, the “Ocular Phenotyping Core” was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Optomotry optokinetic nystagmus in small animals including rats, mice and zebrafish, and Spectralis SDOCT for large animals and human donor eyes. An S10 grant awarded to Dr. Paul Gamlin in 2020 has allowed the purchase of three new tools that will greatly enhance ocular phenotyping of both large and small animal tissues 1) Zeiss Lumera 700 ReScan with Resight 700 operating microscope with intraoperative optical coherence tomography (OCT) imaging; 2) FLEX Module Spectralis OCT2 System for imaging the retina and optic nerve head with structural and angiographic OCT, in animals at various body positions; 3) Anterior Segment CASIA SS-1000 swept source OCT (Tomey Corp.) for imaging the cornea, iris, and lens in large animals, as well as the whole eye in small animals. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive all of whom are currently NEI R01-funded. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core will include the completion of a new LED-based ERG system that will simplify this testing. Further, an existing AOSLO system originally designed for human retinal imaging will be re-engineered in order to image rodent and tree shrew eyes in vivo. At the same time, capabilities for fluorescence imaging and cell-targeted photo-stimulation will be added. Additionally, Ocular Phenotyping Opportunities will be advertised to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists whose animals were originally generated to answer questions pertinent to their organ systems of interest.