Cross-kingdom RNA communications between plant and fungal pathogens
植物和真菌病原体之间的跨界RNA通讯
基本信息
- 批准号:10687042
- 负责人:
- 金额:$ 37.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-15 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimalsAntifungal AgentsArabidopsisBiochemicalBiological ModelsBotrytisCellsCommunicationDiseaseExposure toFriendsGene SilencingGenesGeneticGenomicsHealthHost DefenseHumanImmune responseImmunityInfectionMammalsMessenger RNAModelingMoldsMolecular BiologyParasitesPlantsProductionRNARNA InterferenceRNA TransportSmall RNASortingSystemTravelUntranslated RNAVesicleVirulencedesigndisorder controlextracellular vesiclesfungicideinfancyinnovationinsightpathogenpathogenic funguspathogenic microbeuptake
项目摘要
SUMMARY
Fungal pathogens infect humans, animals, and plants and cause severe consequences on
global human health and crop production. Communication between hosts and pathogens is
essential for host defense and pathogen virulence, but the underlying mechanisms are not
well understood. Previous studies in my lab discovered that some non-coding regulatory
small RNAs (sRNAs) from fungal pathogens, such as Botrytis cinerea, which causes grey
mold disease on more than 1000 plant species, can be transported into host plant cells and
suppress host immunity genes, a mechanism called “Cross-Kingdom RNAi”. Recently, we
discovered that such sRNA communication is bi-directional. Plant hosts have also
developed the ability to deliver sRNAs, mainly using extracellular vesicles, to fungal cells
and induce cross-kingdom RNAi of fungal virulence-related genes. Such sRNA
communication was also observed between mammals and parasites. Although more and
more studies across diverse systems demonstrate that mobile sRNAs are key regulatory
molecules in host and pathogen interactions, the field of cross-kingdom/cross-species RNA
communication is still in its infancy. This proposal is designed to use plant Arabidopsis
and fungal pathogen Botrytis as a model system to address the outstanding questions in this
field, including, how host cells control sRNA transport upon infection, how specific classes
of small RNAs are sorted into extracellular vesicles, how fungal cells deliver sRNAs into
host cells, what are the mechanisms of RNA and vesicle uptake in the host cells and fungal
cells, and whether other classes of RNAs, such as mRNAs and long non-coding RNAs,
move between host and fungal cells, how they function in the counter party, etc.. A
combination of genetics, genomics, biochemical and molecular biology approaches will be
used. This project is expected to provide unprecedented insight into the underlying
mechanisms of cross-kingdom/cross-species RNA communications, which will ultimately
help develop innovative and eco-friendly disease control strategies and RNA-based
fungicides or antifungal drugs.
总结
真菌病原体感染人类,动物和植物,并对人类造成严重后果。
全球人类健康和作物生产。宿主和病原体之间的通讯是
对宿主防御和病原体毒力至关重要,但其潜在机制不是
很好理解。我实验室以前的研究发现,一些非编码调节
来自真菌病原体的小RNA(sRNA),如灰葡萄孢,它会导致灰色
霉菌病在超过1000种植物上,可以被运送到宿主植物细胞中,
抑制宿主免疫基因,这种机制被称为“跨王国RNAi”。最近我们
发现这种sRNA通讯是双向的。植物宿主也有
开发了主要使用细胞外囊泡将sRNA递送到真菌细胞的能力
并诱导真菌毒力相关基因的跨界RNAi。这种sRNA
哺乳动物和寄生虫之间也有交流。虽然越来越
在不同系统中的更多研究表明,移动的sRNA是关键的调控因子,
宿主和病原体相互作用中的分子,跨王国/跨物种RNA领域
通信仍处于起步阶段。本提案旨在利用植物拟南芥
和真菌病原体葡萄孢属作为一个模型系统,以解决这一悬而未决的问题,
领域,包括,宿主细胞如何控制感染后的sRNA运输,如何特定的类
的小RNA被分选到细胞外囊泡中,真菌细胞如何将sRNA递送到
宿主细胞,RNA和囊泡在宿主细胞和真菌中的摄取机制是什么?
细胞,以及其他类型的RNA,如mRNA和长非编码RNA,
在宿主和真菌细胞之间移动,它们如何在对方中发挥作用,等等。一
遗传学、基因组学、生物化学和分子生物学方法的结合将是
采用该项目预计将提供前所未有的深入了解的基础
跨王国/跨物种RNA通讯的机制,这将最终
帮助开发创新和生态友好的疾病控制策略,
杀真菌剂或抗真菌药物。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Hailing Jin其他文献
Hailing Jin的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Hailing Jin', 18)}}的其他基金
Cross-kingdom RNA communications between plant and fungal pathogens
植物和真菌病原体之间的跨界RNA通讯
- 批准号:
10261357 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Cross-kingdom RNA communications between plant and fungal pathogens
植物和真菌病原体之间的跨界RNA通讯
- 批准号:
10468983 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Cross-kingdom RNA communications between plant and fungal pathogens
植物和真菌病原体之间的跨界RNA通讯
- 批准号:
10797921 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Modification and dynamic regulation of Argonaute proteins-the key small RNA pathway components in host innate immunity
Argonaute蛋白的修饰和动态调控——宿主先天免疫中关键的小RNA通路成分
- 批准号:
9231457 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
Modification and dynamic regulation of Argonaute proteins-the key small RNA pathway components in host innate immunity
Argonaute蛋白的修饰和动态调控——宿主先天免疫中关键的小RNA通路成分
- 批准号:
9030945 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
The role of host endogenous small RNAs in innate immunity
宿主内源小RNA在先天免疫中的作用
- 批准号:
8499367 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
The role of host endogenous small RNAs in innate immunity
宿主内源小RNA在先天免疫中的作用
- 批准号:
8280395 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
The role of host endogenous small RNAs in innate immunity
宿主内源小RNA在先天免疫中的作用
- 批准号:
8073552 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
The role of host endogenous small RNAs in innate immunity
宿主内源小RNA在先天免疫中的作用
- 批准号:
8728936 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
The role of host endogenous small RNAs in innate immunity
宿主内源小RNA在先天免疫中的作用
- 批准号:
7865684 - 财政年份:2010
- 资助金额:
$ 37.73万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 37.73万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 37.73万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 37.73万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 37.73万 - 项目类别:
Discovery Early Career Researcher Award
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Continuing Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Analysis of thermoregulatory mechanisms by the CNS using model animals of female-dominant infectious hypothermia
使用雌性传染性低体温模型动物分析中枢神经系统的体温调节机制
- 批准号:
23KK0126 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Fund for the Promotion of Joint International Research (International Collaborative Research)
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 37.73万 - 项目类别:
Training Grant