Mitochondrial Imaging, PTM Analysis and Metabolism Core
线粒体成像、PTM 分析和代谢核心
基本信息
- 批准号:10705684
- 负责人:
- 金额:$ 33.17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-20 至 2025-07-31
- 项目状态:未结题
- 来源:
- 关键词:AnimalsApoptoticArizonaBiochemicalBioenergeticsBiologicalBiological AssayBiophysicsBlack raceBlood flowCarnitineCellsCellular Metabolic ProcessChildComplex AnalysisCore FacilityCoupledCultured CellsDataDedicationsDetectionEndotheliumEnsureEquipmentGlycolysisGoalsHuman ResourcesImageIn VitroIndividualKineticsLaboratoriesLungMass Spectrum AnalysisMeasurementMechanical StressMembrane PotentialsMetabolicMetabolismMitochondriaModificationMolecularOxygen ConsumptionPhenotypePhysiologicalPlasmaPost-Translational Protein ProcessingPreparationProceduresProcessProductionProtein AnalysisProteinsPyruvateReactive Oxygen SpeciesReproducibilityResearch PersonnelResolutionResourcesRunningSamplingServicesSiteStandardizationStructureStructure of parenchyma of lungSystemTechniquesTissuesTrainingUniversitiesWorkcongenital heart disordercost efficientexperiencefatty acid oxidationhigh resolution imagingin vivomechanotransductionmetabolomicsmitochondrial membranepulmonary vascular disordersuccesstandem mass spectrometry
项目摘要
ABSTRACT
The goal of the Mitochondrial Imaging, PTM analysis, and Metabolism Core (Core B) is to provide a high-
quality state-of-the art service to support analyses in all three of the PPG Projects. In this context, Core B will
be defined by providing four services. 1) High resolution imaging of mitochondria. This will include
analyses of specific protein redistribution to mitochondria, mitochondrial network remodeling, and markers
of mitochondrial function (mitochondrial derived reactive oxygen species, mitochondrial membrane
potential, carnitine metabolites, ATP levels). 2) Bioenergetics: oxygen consumption, rate of glycolysis, fatty
acid oxidation, measurement of cellular ATP kinetics, ETC complex analyses in mechanical stress
challenged cells. 3) The analysis of PTMs in biological samples. Individual proteins will be purified to assay
PTMs in vivo. To analyze PTMs, we will utilize a mass spectrometry approach coupled to the detection and
quantification of specific sites of modification by tandem mass spectrometry. 4) Metabolomic analyses of
individual metabolites in cultured cells, lung tissue, and plasma from animals and children born with
congenital heart disease that results in changes in pulmonary blood flow (PBF). Core B will enhance the
scientific work for all three projects by assuring that all mitochondrial, PTM, and metabolomic analyses
are standardized and performed in a uniform standardized manner, and by highly experienced and
trained personnel. Core B is run by experienced investigators who have had many years of experience
using these techniques. They have developed or adapted many of these techniques and applied them to
perform physiological studies in in vitro and in vivo systems. As a central dedicated analytical resource Core
B will ensure these analyses are carried out in a cost-efficient manner.
摘要
线粒体成像、PTM分析和代谢核心(核心B)的目标是提供高-
优质的最先进的服务,以支持所有三个PPG项目的分析。在这种情况下,核心B将
通过提供四项服务来定义。1)线粒体高分辨率成像。这将包括
线粒体特异性蛋白质重分布、线粒体网络重构和标志物的分析
线粒体功能(线粒体衍生的活性氧物种、线粒体膜
潜力、肉碱代谢物、三磷酸腺苷水平)。2)生物能量学:耗氧量、糖酵解率、脂肪
机械应力中的酸氧化、细胞ATP动力学测定等综合分析
挑战细胞。3)生物样品中PTMS的分析。单独的蛋白质将被提纯以进行检测
体内的PTMS。为了分析PTMS,我们将利用质谱学方法结合检测和
串联质谱法对修饰的特定部位进行定量。4)新陈代谢分析
来自动物和儿童的培养细胞、肺组织和血浆中的个别代谢物
导致肺血流量(PBF)改变的先天性心脏病。核心B将加强
通过确保所有线粒体、PTM和代谢组学分析
都是标准化的,并以统一标准化的方式执行,并由经验丰富的
训练有素的人员。核心B由有多年经验的有经验的调查人员负责
使用这些技术。他们已经开发或调整了其中的许多技术,并将它们应用于
在体外和体内系统中进行生理学研究。作为中央专用分析资源核心
B将确保以具有成本效益的方式进行这些分析。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Stephen M Black其他文献
Increased NADPH Oxidase Derived Superoxide is Involved in Hypoxia Ischemic Neuronal Cell Death in Neonatal Hippocampal Slice Cultures
- DOI:
10.1016/j.freeradbiomed.2010.10.440 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Qing Lu;Valerie Harris;Yali Hou;Thomas Rau;Jing Tian;David J Poulsen;Stephen M Black - 通讯作者:
Stephen M Black
Endothelin-1 in Congenital Heart Disease
内皮素-1 在先天性心脏病中
- DOI:
10.1203/01.pdr.0000160447.83332.13 - 发表时间:
2005-05-01 - 期刊:
- 影响因子:3.100
- 作者:
Maurice Beghetti;Stephen M Black;Jeffrey R Fineman - 通讯作者:
Jeffrey R Fineman
206 - Targeted Protein Protection from Oxidative/ Nitrosative Post-Translational Modifications Using Shielding Peptides
- DOI:
10.1016/j.freeradbiomed.2015.10.250 - 发表时间:
2015-10-01 - 期刊:
- 影响因子:
- 作者:
Olga Rafikova;Stephen M Black;Ruslan Rafikov - 通讯作者:
Ruslan Rafikov
The Rebound Pulmonary Hypertension Associated with the Acute Withdrawal of Inhaled Nitric Oxide involves the Nitration-Mediated Inhibition of Protein Kinase G: Protective Role of Superoxide Dismutase
- DOI:
10.1016/j.freeradbiomed.2010.10.002 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Saurabh Aggarwal;Christine M Gross;Sanjiv Kumar;Peter Oishi;Jeffrey R Fineman;Stephen M Black - 通讯作者:
Stephen M Black
Nitration-mediated Activation of RhoA: Its Role in LPS-induced Endothelial Barrier Dysfunction
- DOI:
10.1016/j.freeradbiomed.2010.10.106 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Ruslan Rafikov;Saurabh Aggarwal;Christine Gross;Sanjiv Kumar;Yali Hou;Connie Snead;Agnieszka Jezierska;Alexander D Verin;David Fulton;John Catravas;Stephen M Black - 通讯作者:
Stephen M Black
Stephen M Black的其他文献
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{{ truncateString('Stephen M Black', 18)}}的其他基金
Genetics, Epigenetics, and Post-translational Modifications and the Development of Ventilator Induced Lung Injury (VILI)
遗传学、表观遗传学和翻译后修饰以及呼吸机所致肺损伤 (VILI) 的发生
- 批准号:
10455906 - 财政年份:2021
- 资助金额:
$ 33.17万 - 项目类别:
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