Are all Protein Aggregates Toxic?

所有蛋白质聚集体都有毒吗？

• 批准号: 10798900
• 负责人: Ashutosh Tiwari
• 金额: $ 10万
• 依托单位: MICHIGAN TECHNOLOGICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-22 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798900
• 关键词: Address; Administrative Supplement; Biochemical; Biophysics; Chromatography; Circular Dichroism; Eligibility Determination; Fluorescence; Funding; Grant; Methods; Michigan; Property; Research; Secondary Protein Structure; Structure; Techniques; Time; Toxic effect; instrument; protein aggregation; response

This application is in response to NOSI (NOT-GM-22-017) to acquire a Circular Dichroism Spectropolarimeter as part of an administrative supplement. PI has an active eligible R15 grant (1 R15GM148964-01) with enough remaining time to proceed with a supplement request. Currently, Michigan Tech does not have a CD instrument. Having access to this instrument will greatly enhance PI’s research ability to address specific aim 2 (Characterize physicochemical properties of the diverse protein aggregates) of the funded grant (1 R15GM148964-01). Circular Dichroism can track changes in the secondary structure of proteins undergoing misfolding and aggregation. This technique – in combination with other biophysical and biochemical methods such as chromatography, fluorescence, SEM, AFM, and TEM – will help to better characterize the unique physicochemical properties of the protein aggregates and correlate aggregates’ structure to its associated toxicity.

本申请是对NOSI（NOT-GM-22-017）的响应，以获得圆二色性 作为行政补助的一部分。PI拥有有效的合格R15补助金 （1 R15 GM 148964 -01），有足够的剩余时间继续处理补充请求。 目前，密歇根理工大学没有CD仪器。使用该仪器将 大大提高PI的研究能力，以解决具体目标2（表征理化 不同蛋白质聚集体的性质）的资助（1 R15 GM 148964 -01）。 圆二色性可以跟踪蛋白质二级结构的变化， 错误折叠和聚集。这种技术-结合其他生物物理和 生物化学方法，如色谱法，荧光，SEM，AFM和TEM -将有助于 为了更好地表征蛋白质聚集体的独特物理化学性质， 将聚集体的结构与其相关毒性联系起来。