Are all Protein Aggregates Toxic?

所有蛋白质聚集体都有毒吗？

• 批准号: 10798900
• 负责人: Ashutosh Tiwari
• 金额: $ 10万
• 依托单位: MICHIGAN TECHNOLOGICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-22 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798900
• 关键词: Address; Administrative Supplement; Biochemical; Biophysics; Chromatography; Circular Dichroism; Eligibility Determination; Fluorescence; Funding; Grant; Methods; Michigan; Property; Research; Secondary Protein Structure; Structure; Techniques; Time; Toxic effect; instrument; protein aggregation; response

This application is in response to NOSI (NOT-GM-22-017) to acquire a Circular Dichroism Spectropolarimeter as part of an administrative supplement. PI has an active eligible R15 grant (1 R15GM148964-01) with enough remaining time to proceed with a supplement request. Currently, Michigan Tech does not have a CD instrument. Having access to this instrument will greatly enhance PI’s research ability to address specific aim 2 (Characterize physicochemical properties of the diverse protein aggregates) of the funded grant (1 R15GM148964-01). Circular Dichroism can track changes in the secondary structure of proteins undergoing misfolding and aggregation. This technique – in combination with other biophysical and biochemical methods such as chromatography, fluorescence, SEM, AFM, and TEM – will help to better characterize the unique physicochemical properties of the protein aggregates and correlate aggregates’ structure to its associated toxicity.

此应用是响应NOSI（NOT-GM-22-017）以获取圆形二分的 光谱仪作为行政补充的一部分。 PI有一个积极的合格R15赠款 （1 R15GM148964-01）有足够的剩余时间来进行补充请求。 目前，密歇根理工学院没有CD仪器。访问该乐器将 极大地增强了PI的研究能力，以解决特定目标2（表征物理 资助赠款（1 R15GM148964-01）的潜水蛋白聚集体的性质。 圆二色性可以跟踪正在进行的蛋白质的二级结构的变化 错误折叠和聚合。这种技术 - 与其他生物物理和 色谱，荧光，SEM，AFM和TEM等生化方法将有助于 更好地表征蛋白质聚集体的独特物理特性和 将聚集体的结构与其相关毒性相关联。