Are all Protein Aggregates Toxic?

此应用程序响应于NOSI(非-GM-22-017)来获得圆二向色性 作为行政补充的一部分的光谱偏振仪。PI具有有效的合格R15奖励 (1 R15GM148964-01)，并有足够的剩余时间继续提出补充请求。 目前，密歇根理工大学没有CD乐器。能够接触到这个工具将会 极大地增强了PI针对特定目标的研究能力2(表征物理化学 不同蛋白质聚集体的性质)的资助(1 R15GM148964-01)。 圆二色谱可以跟踪蛋白质二级结构的变化 错误折叠和聚合。这项技术-结合其他生物物理和 生物化学方法，如层析、荧光、扫描电子显微镜、原子力显微镜和透射电子显微镜-将有所帮助 为了更好地表征蛋白质聚集体的独特物理化学性质和 将聚集体的结构与其相关的毒性联系起来。

This application is in response to NOSI (NOT-GM-22-017) to acquire a Circular Dichroism Spectropolarimeter as part of an administrative supplement. PI has an active eligible R15 grant (1 R15GM148964-01) with enough remaining time to proceed with a supplement request. Currently, Michigan Tech does not have a CD instrument. Having access to this instrument will greatly enhance PI’s research ability to address specific aim 2 (Characterize physicochemical properties of the diverse protein aggregates) of the funded grant (1 R15GM148964-01). Circular Dichroism can track changes in the secondary structure of proteins undergoing misfolding and aggregation. This technique – in combination with other biophysical and biochemical methods such as chromatography, fluorescence, SEM, AFM, and TEM – will help to better characterize the unique physicochemical properties of the protein aggregates and correlate aggregates’ structure to its associated toxicity.