Charge Transfer Study of DNA/MoS2 interface

DNA/MoS2界面的电荷转移研究

• 批准号: 10798439
• 负责人: Mohtashim H Shamsi
• 金额: $ 9.97万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798439
• 关键词: Affect; Affinity; Atomic Force Microscopy; Award; Base Pairing; Basic Science; Behavior; Charge; Chemistry; Complement; DNA; Detection; Disease; Disulfides; Future; Grant; Individual; Investigation; Label; Length; Measurement; Microscopy; Molecular Conformation; Molybdenum; Mutation; Nucleotides; Parents; Persons; Property; Proteins; Research; Research Personnel; Scanning Probe Microscopes; Science; Signal Transduction; Surface; Techniques; Technology; Theoretical Studies; Training; Trinucleotide Repeats; detection platform; diagnostic technologies; experimental study; genetic testing; materials science; nanopore; nucleobase; undergraduate student

Project Summary Expansion of tandem DNA repeats—trinucleotide repeats or TNR—cause more than fifty genetically transferrable disorders, which affect 4 million people every year. Current state-of-the-art diagnostic technologies for genetic testing for length mutations have their own limitations such as clogging of protein nanopores, requiring labelling steps, frequent false positive/negative results, or short basepair read length. To overcome the limitations that hamper the current biomedical science, there is a critical need to develop new platforms standing on rigorous basic science. The parent AREA award involves mainly undergraduate researchers to investigate intrinsic charge transfer character of tandem DNA repeats interfaced with MoS2 surfaces that may ultimately manifest into a label-free sensing platform in future. The project urgently needs a conductive atomic force microscope (C-AFM) to study the electrical charge transfer property of the interface to complement and validate electrochemical charge transfer behavior. Leveraging on theoretical and experimental studies, the PI hypothesizes that DNA repeats can produce sequence- and length-dependent label-free charge transfer signals due to the differential affinity of the nucleobases for molybdenum disulfide (MoS2). This is critical to study through a rigorous plan as detailed in the parent award. Currently, we are performing electrochemical measurements that have to be confirmed by surface probe microscopy techniques, such as C-AFM. In the specific aims, the PI plans to study 66 sequences of two different lengths and specifically (1) investigate sequence- dependent charge transport at TNR/MoS2 interface, and (2) investigate length-dependent charge transport at the interface. The experiments also involve studying the effects of sequence concentration and conformations. This supplement grant will train the undergraduate researchers on C-AFM technique which is a powerful surface probe technique used in surface chemistry and materials science researches. Ultimately, this supplement award will substantiate our electrochemical study in the parent award and provide a critical piece of information to develop a new and sensitive technology for the detection of length mutations.

项目摘要 串联DNA重复序列的扩增-三核苷酸重复序列或TNR-导致50多个 遗传性疾病，每年影响400万人。目前最先进的技术 用于长度突变的基因检测的诊断技术具有其自身的局限性， 蛋白质纳米孔堵塞，需要标记步骤，频繁的假阳性/阴性结果，或短时间 碱基对读取长度。为了克服阻碍当前生物医学科学的局限性， 迫切需要开发基于严格基础科学的新平台。家长区奖 主要涉及本科研究人员，以研究串联的固有电荷转移特性 与MoS 2表面连接的DNA重复序列最终可能表现为无标记传感 未来的平台该项目迫切需要一种导电原子力显微镜（C-AFM）进行研究 界面的电荷转移特性，以补充和验证电化学 电荷转移行为 利用理论和实验研究，PI假设DNA重复可以 产生序列和长度相关的无标记电荷转移信号， 核碱基对二硫化钼（MoS 2）的亲和力。这对于通过严格的 详细的计划在父母的奖励。目前，我们正在进行电化学测量， 必须通过表面探针显微镜技术，如C-AFM证实。在具体目标上， PI计划研究两种不同长度的66个序列，具体而言，（1）研究序列- 在TNR/MoS 2界面依赖的电荷输运，和（2）研究长度依赖的电荷 在接口处的传输。实验还涉及研究序列浓度的影响 和构象。 该补助金将用于培养C-AFM技术的本科研究人员， 强大的表面探针技术用于表面化学和材料科学研究。 最终，这个补充奖将证实我们在母公司奖的电化学研究， 提供了一个关键的信息，以开发一种新的和敏感的技术，用于检测 长度突变。