Charge Transfer Study of DNA/MoS2 interface

DNA/MoS2界面的电荷转移研究

• 批准号: 10798439
• 负责人: Mohtashim H Shamsi
• 金额: $ 9.97万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798439
• 关键词: Affect; Affinity; Atomic Force Microscopy; Award; Base Pairing; Basic Science; Behavior; Charge; Chemistry; Complement; DNA; Detection; Disease; Disulfides; Future; Grant; Individual; Investigation; Label; Length; Measurement; Microscopy; Molecular Conformation; Molybdenum; Mutation; Nucleotides; Parents; Persons; Property; Proteins; Research; Research Personnel; Scanning Probe Microscopes; Science; Signal Transduction; Surface; Techniques; Technology; Theoretical Studies; Training; Trinucleotide Repeats; detection platform; diagnostic technologies; experimental study; genetic testing; materials science; nanopore; nucleobase; undergraduate student

Project Summary Expansion of tandem DNA repeats—trinucleotide repeats or TNR—cause more than fifty genetically transferrable disorders, which affect 4 million people every year. Current state-of-the-art diagnostic technologies for genetic testing for length mutations have their own limitations such as clogging of protein nanopores, requiring labelling steps, frequent false positive/negative results, or short basepair read length. To overcome the limitations that hamper the current biomedical science, there is a critical need to develop new platforms standing on rigorous basic science. The parent AREA award involves mainly undergraduate researchers to investigate intrinsic charge transfer character of tandem DNA repeats interfaced with MoS2 surfaces that may ultimately manifest into a label-free sensing platform in future. The project urgently needs a conductive atomic force microscope (C-AFM) to study the electrical charge transfer property of the interface to complement and validate electrochemical charge transfer behavior. Leveraging on theoretical and experimental studies, the PI hypothesizes that DNA repeats can produce sequence- and length-dependent label-free charge transfer signals due to the differential affinity of the nucleobases for molybdenum disulfide (MoS2). This is critical to study through a rigorous plan as detailed in the parent award. Currently, we are performing electrochemical measurements that have to be confirmed by surface probe microscopy techniques, such as C-AFM. In the specific aims, the PI plans to study 66 sequences of two different lengths and specifically (1) investigate sequence- dependent charge transport at TNR/MoS2 interface, and (2) investigate length-dependent charge transport at the interface. The experiments also involve studying the effects of sequence concentration and conformations. This supplement grant will train the undergraduate researchers on C-AFM technique which is a powerful surface probe technique used in surface chemistry and materials science researches. Ultimately, this supplement award will substantiate our electrochemical study in the parent award and provide a critical piece of information to develop a new and sensitive technology for the detection of length mutations.

项目摘要 串联DNA重复序列的扩展 - 三核苷酸重复或TNR - 由于五十多个 遗传转移的疾病，每年影响400万人。当前最新的 长度突变的基因测试诊断技术具有其自身的局限性，例如 堵塞蛋白质纳米孔，需要标记步骤，经常假阳性/阴性结果或短暂 基础式读取长度。为了克服妨碍当前生物医学科学的局限性，有 建立严格基础科学的新平台的迫切需求。父区奖 主要涉及本科研究人员来研究串联的固有电荷转移特征 DNA重复与MOS2表面相连的DNA，最终可能表现为无标签感应的DNA表面 将来的平台。该项目迫切需要一个导电原子力显微镜（C-AFM）来研究 接口的电荷传输特性补充和验证电子化学 充电转移行为。 利用理论和实验研究，PI假设DNA重复可以 由于差异而产生序列序列和长度依赖性的无标签电荷传输信号 核苷酶对钼二硫化物的亲和力（MOS2）。这对于仔细研究严格 计划在父母奖中详细介绍。目前，我们正在执行电化学测量值 必须通过表面探针显微镜技术（例如C-AFM）确认。在具体目标中 PI计划研究66个不同长度的序列，具体（1）研究序列 - TNR/MOS2界面处的依赖电荷运输，（2）研究长度依赖性电荷 在界面上运输。实验还涉及研究序列浓度的影响 和构象。 该补充补助金将培训本科研究人员的C-AFM技术，这是一种 表面化学和材料科学研究中使用的强大表面探针技术。 最终，该补充奖将在父母奖中证实我们的电化学研究，并证实 提供关键信息，以开发一种新的敏感技术来检测 长度突变。