Charge Transfer Study of DNA/MoS2 interface

DNA/MoS2界面的电荷转移研究

• 批准号: 10514726
• 负责人: Mohtashim H Shamsi
• 金额: $ 42.95万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10514726
• 关键词: Address; Affect; Affinity; Area; Basic Science; Behavior; Binding; Biomedical Research; Biomedical Technology; Characteristics; Charge; Chemistry; DNA; DNA Sequence; DNA sequencing; DNA-Binding Proteins; Data; Detection; Development; Devices; Disease; Disulfides; Electron Transport; Future; GTP-Binding Protein alpha Subunits, Gs; Genes; Goals; Growth; Human; Individual; Investigation; Label; Length; Measurement; Microscopy; Molecular Conformation; Molybdenum; Monitor; Mutation; Nucleotides; Pathologic; Patients; Persons; Positioning Attribute; Proteins; Public Health; Research; Research Personnel; Resistance; Science; Signal Transduction; Single Nucleotide Polymorphism; Students; Supervision; Surface; Techniques; Technology; Testing; Theoretical Studies; Transducers; Trinucleotide Repeats; base; design; detection limit; detection platform; diagnostic technologies; flexibility; genetic testing; graduate student; improved; interfacial; materials science; mutant; nanomaterials; nanopore; nanosheet; nucleobase; tool; two-dimensional; undergraduate student; van der Waals force

Project Summary Expansion of tandem DNA repeats cause more than forty genetically transferrable disorders, which affect 4 million people every year. Current state-of-the-art diagnostic technologies for genetic testing for length mutations have their own limitations such as clogging of protein nanopores, requiring labelling steps, frequent false positive/negative results, or short basepair read length. To overcome the limitations that hamper the current biomedical science, there is a critical need to develop new platforms founded on thorough basic science. This AREA proposal involving mainly undergraduate researchers investigates intrinsic character of tandem DNA repeats interfaced with MoS2 surfaces that may manifest into label-free sensing platform for repeat mutations in future. The PI hypothesizes that DNA repeats can produce sequence- and length-dependent charge transfer signals due to the differential affinity of nucleobases for two-dimensional materials, i.e. molybdenum disulfide (MoS2). This is the critical piece of information needed to confirm through a rigorous study. Based on encouraging preliminary results, current project is designed to fundamentally investigate DNA/MoS2 interfaces in detail by electrochemical and surface probe microscopy techniques. In the specific aims, the PI plans to (1) investigate sequence-dependent charge transport at TNR/2D nanomaterials interface, and (2) investigate their behavior with respect to sequence length. The results will also be compared with concentration and conformations effects on the charge transfer character. This AREA proposal will expose the undergraduate researchers to high-quality research in surface chemistry and materials science, which has ultimate application in biomedical research to improve public health. Upon completion, we will be in better position to apply DNA/2D materials for selective and sensitive detection of repeat mutations, which will ultimately improve the lives of millions of individuals.

项目摘要 串联DNA重复序列的扩展导致了40多种可遗传的疾病， 每年影响400万人。目前最先进的遗传诊断技术 检测长度突变有其自身的局限性，如堵塞蛋白质纳米孔，需要 标记步骤、频繁的假阳性/阴性结果或较短的Basepair读取长度。要克服这个问题 阻碍当前生物医学科学的局限性，迫切需要开发新的平台 建立在彻底的基础科学的基础上。这一领域的建议主要涉及本科生研究人员 研究串联DNA重复序列与可能表现出的MoS2表面的内在特征 转化为无标签的传感平台，用于未来的重复突变。 PI假设DNA重复可以产生序列和长度相关的电荷 由于核碱基对二维材料的不同亲和力而产生的传递信号，即 二硫化钼(MoS2)。这是关键的信息，需要通过 严谨的学习。基于令人鼓舞的初步结果，目前的项目旨在从根本上 用电化学和表面探针显微镜详细研究DNA/MoS2界面 技巧。在特定的目标中，PI计划(1)研究依赖于序列的电荷传输 TNR/2D纳米材料界面，以及(2)研究它们在序列长度方面的行为。这个 结果还将与浓度和构象对电荷转移的影响进行比较 性格。 这一领域的建议将使本科生研究人员接触到 表面化学和材料科学，它在生物医学研究中具有最终的应用 改善公共卫生。完成后，我们将更好地将DNA/2D材料应用于 选择性和灵敏地检测重复突变，这最终将改善数百万人的生活 关于个人的。