Replacement of Fluorescence Imaging System
更换荧光成像系统
基本信息
- 批准号:10797441
- 负责人:
- 金额:$ 10.7万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:AdsorptionAgingBiological AssayBiological ModelsCell AgingCell Cycle ProgressionCellsChromatinChromosomal BreaksChromosome PairingChromosome SegregationChromosome StructuresChromosomesCongenital AbnormalityCruciform DNACytologyDNADNA LigationDNA SequenceDefectDevelopmentDiagnosisDigestionDiseaseElementsEngineeringEnsureExcisionFailureFluorescence MicroscopyFrequenciesFundingGene DosageGene ExpressionGenomeGenome MappingsGenome StabilityGenome engineeringGenomic InstabilityGenomic SegmentGerm LinesGleanHaploidyHeadHealthHi-CHomologous GeneLigationLinkMalignant NeoplasmsMapsMediatingMeiosisMolecularMonitorNucleotidesOrganismPathologicPremature aging syndromeProcessProteinsRefractoryReporterResearchResolutionRibosomal DNASaccharomycetalesSexual ReproductionSister ChromatidSiteSomatic CellSpecificitySurfaceSystemTrans-ActivatorsUpdateWorkYeastsassay developmentchromosome conformation capturechromosome missegregationds-DNAfluorescence imagingfluorescence microscopegenome-widegenome-wide analysishomologous recombinationimaging systemimprovedinsightparent grantpreventprogramsprototyperepairedreproductivetoolwhole genomeyeast genome
项目摘要
PROJECT SUMMARY
The central aim of the parent grant is to develop an assay for genome-wide identification of pairing
interactions between homologous DNA segments. Juxtaposition of homologous DNA sequences is a key
determinant of chromosome structure, genome stability and gene expression control. In somatic cells,
pairing between sister chromatids ensures accurate chromosome break repair and chromosome
segregation. In the germ line, pairing between homologous chromosomes ensures genome haploidization
for sexual reproduction. During development, interactions between homologous chromosome segments
are required for establishing monoallelic gene expression. Pairing defects are associated with
chromosome missegregation, genome rearrangements and aberrant gene dosage, resulting in birth
defects, cancer, and premature aging. Developing an assay that identifies genome segments involved in
homologous pairing is an essential step for a mechanistic understanding of this process. In the parent
grant, we are developing the Homolog Pairing Capture assay, to quantitatively detect homologous DNA
interactions. While applicable to a large number of developmental stages and organisms, we are using
budding yeast meiosis as a model system for assay development. Synchronous meiosis can be induced in
yeast cultures in millions of cells, facilitating the capture of cells at the stage of genome-wide homologous
pairing. Many of our approaches involve monitoring of meiotic cell cycle progression via fluorescence
microscopy. In this supplement proposal, we are requesting funds for a replacement for our aging routine
fluorescence microscope and a sensitive camera integrated with an up-to-date imaging system. Our
current system has become unreliable and is near the end of its lifetime. An updated fluorescence
microscope will enable us to maintain and further enhance a high-impact research program into
mechanisms of homologous chromosome pairing.
项目总结
父母基金的中心目标是开发一种全基因组范围内鉴定配对的方法。
同源DNA片段之间的相互作用。同源DNA序列的并列是一个关键
染色体结构、基因组稳定性和基因表达调控的决定因素。在体细胞中,
姐妹染色单体之间的配对确保了准确的染色体断裂修复和染色体
种族隔离。在生殖系中,同源染色体之间的配对确保了基因组单倍化。
用于有性繁殖。在发育过程中,同源染色体片段之间的相互作用
是建立单等位基因表达所必需的。配对缺陷与
染色体错误分离、基因组重排和基因剂量异常,导致出生
缺陷、癌症和过早衰老。开发一种检测方法,以识别涉及到
同源配对是从机制上理解这一过程的关键一步。在父级中
格兰特,我们正在开发同源配对捕获试验,以定量检测同源DNA
互动。虽然适用于大量的发育阶段和生物体,但我们正在使用
芽殖酵母减数分裂作为检测发展的模式系统。同步性减数分裂可诱导
酵母在数百万个细胞中培养,促进在全基因组同源阶段捕获细胞
配对。我们的许多方法包括通过荧光监测细胞减数分裂周期进程
显微镜。在这份补充提案中,我们申请资金来替换我们老化的例行公事
荧光显微镜和集成了最新成像系统的灵敏相机。我们的
目前的系统已经变得不可靠,即将结束其生命周期。一种更新的荧光
显微镜将使我们能够保持并进一步增强一个高影响力的研究计划,以
同源染色体配对的机制。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Valentin Boerner其他文献
Valentin Boerner的其他文献
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{{ truncateString('Valentin Boerner', 18)}}的其他基金
Assay for Detection of Homologous DNA Interactions
同源 DNA 相互作用的检测分析
- 批准号:
10366921 - 财政年份:2022
- 资助金额:
$ 10.7万 - 项目类别:
Assay for Detection of Homologous DNA Interactions
同源 DNA 相互作用的检测分析
- 批准号:
10614927 - 财政年份:2022
- 资助金额:
$ 10.7万 - 项目类别:
Control of pairing and recombination during meiosis
减数分裂过程中配对和重组的控制
- 批准号:
8180448 - 财政年份:2011
- 资助金额:
$ 10.7万 - 项目类别:
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