How bacterial SMC complexes organize chromosomes

细菌 SMC 复合体如何组织染色体

• 批准号: 10797569
• 负责人: Xindan Wang
• 金额: $ 1.6万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797569
• 关键词: Administrative Supplement; Bacteria; Biochemical; Cell Cycle; Cells; Chromosome Segregation; Chromosome Structures; Chromosomes; Complex; Cytology; Equipment; Funding; Genetic; Goals; Human; Maintenance; Molecular; Organism; Reagent; Research; Sampling; chromosome replication; cold temperature; condensin; experimental study; genome integrity; parent grant; segregation

Project Summary The primary goal of our funded research (1R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. Our research requires a lot of samples to be stored at -80˚C. However, our existing -80˚C is full of essential reagents and we are in need of a new freezer. In this application for Administrative Supplements for Equipment, I am requesting a Cryocube F740HI Freezer, which will allow us to continue to perform the experiments proposed in the parent grant.

项目概要 我们资助的研究 (1R01GM141242) 的主要目标是了解染色体是如何组织的 并在细胞周期中分离。我们重点研究SMC凝缩蛋白复合物的分子机制， 是从细菌到人类染色体动力学的关键参与者。我们的项目结合了遗传、分子、 细胞学和生化方法。我们的研究需要大量样品在-80℃下保存。然而， 我们现有的 -80°C 充满了必需的试剂，我们需要一个新的冰箱。在本申请中 设备管理补充资料，我请求一台 Cryocube F740HI 冷冻机，这将使我们能够 继续进行父资助中提议的实验。