How bacterial SMC complexes organize chromosomes

项目摘要 我们资助的研究（1 R 01 GM 141242）的主要目标是了解染色体是如何组织的 并在细胞周期中分离。我们重点研究SMC凝聚复合物的分子机制， 是从细菌到人类染色体动力学的关键角色。我们的项目结合了联合收割机基因，分子， 细胞学和生物化学方法。我们的研究需要大量的样本储存在-80杰。然而，在这方面， 我们现有的-80杰C装满了必要的试剂，我们需要一个新的冷冻箱。本申请中为了 设备的行政补充，我要求一个Cryocube F740 HI冷冻机，这将使我们能够 继续进行父母补助金中提出的实验。

Project Summary The primary goal of our funded research (1R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. Our research requires a lot of samples to be stored at -80˚C. However, our existing -80˚C is full of essential reagents and we are in need of a new freezer. In this application for Administrative Supplements for Equipment, I am requesting a Cryocube F740HI Freezer, which will allow us to continue to perform the experiments proposed in the parent grant.