How bacterial SMC complexes organize chromosomes

项目摘要 我们资助的研究(1R01GM141242)的主要目标是了解染色体是如何组织的 并在细胞周期中被分离。我们重点研究了SMC凝集素复合体的分子机制。 是从细菌到人类的染色体动力学的关键参与者。我们的项目结合了遗传、分子、 细胞学和生化方法。我们的研究需要将大量样品储存在-80˚C下，然而， 我们现有的-80˚C充满了必要的试剂，我们需要一个新的冰柜。在此应用程序中， 设备的行政补充，我要求一台Cryoccube F740HI冷冻机，它将允许我们 继续进行家长拨款中提出的实验。

Project Summary The primary goal of our funded research (1R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. Our research requires a lot of samples to be stored at -80˚C. However, our existing -80˚C is full of essential reagents and we are in need of a new freezer. In this application for Administrative Supplements for Equipment, I am requesting a Cryocube F740HI Freezer, which will allow us to continue to perform the experiments proposed in the parent grant.