How bacterial SMC complexes organize chromosomes (Equipment Supplement)

项目摘要 我们资助的研究(1R01GM141242)的主要目标是了解染色体是如何组织的 并在细胞周期中被分离。我们重点研究了SMC凝集素复合体的分子机制。 是从细菌到人类的染色体动力学的关键参与者。我们的项目结合了遗传、分子、 细胞学和生化方法。所有这些方法都需要我们将细菌细胞培养到 所需的实验条件。在这份设备行政补充申请中，我是 请求新的Brunswick Innova 44R系统，它将允许我们继续培养细胞以执行 家长拨款中提出的实验。

Project Summary The primary goal of our funded research (1R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. All of these approaches require that we grow the bacterial cells to the desired experimental conditions. In this application for Administrative Supplements for Equipment, I am requesting the New Brunswick Innova 44R system, which will allow us to continue growing cells to perform the experiments proposed in the parent grant.