Structural and functional determinants of decision-making in bacteriophage host recognition

噬菌体宿主识别决策的结构和功能决定因素

• 批准号: 10798641
• 负责人: Petr G Leiman
• 金额: $ 14.27万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-10 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10798641
• 关键词: Acceleration; Administrative Supplement; Anions; Antibiotic Therapy; Bacteriophages; Biological Models; Biophysics; Cell surface; Cells; Column Chromatography; Complex; Cryoelectron Microscopy; Decision Making; Diagnostic; Exclusion; Funding; High Pressure Liquid Chromatography; Individual; Liquid Chromatography; Membrane Proteins; Modeling; Molecular Conformation; Molecular Weight; National Institute of General Medical Sciences; O Antigens; Oligonucleotides; Organelles; Penetration; Polysaccharides; Preparation; Process; Research Personnel; Resolution; Role; Structure; System; Therapeutic; United States National Institutes of Health; Viral; Virion; Virus; Work; X-Ray Crystallography; antibiotic resistant infections; cell envelope; equipment acquisition; macromolecule; parent grant; particle; public health relevance; response; success; tool; transforming virus

PROJECT SUMMARY/ABSTRACT In response to NOT-GM-22-017, Notice of Availability of Administrative Supplements for Equipment Purchases for NIGMS Awardees, we request the funds to purchase one ÄKTA pure 25 M liquid chromatography system and a set of chromatography columns most suitable for the work proposed in the parent grant R01GM139034. The parent grant aims to establish the mechanisms by which viruses recognize their host cells, undergo a conformational change, and irreversibly attach to the cell surface. Using several bacterial viruses (bacteriophages) as model systems, the project aims to determine the role of different phage virion components in the attachment process, the energetics of host cell surface recognition, and the structure of the phage particle in the midst of host cell envelope penetration. Individual virion components, viral host- attachment organelles, complete phage particles, and various components of the host cell – oligo- and polysaccharides, including O-antigens, and membrane proteins – will be studied using X-ray crystallography, cryo-electron microscopy, and solution biophysics. High quality and high purity preparations of macromolecules and their complexes are essential for the success of these studies. Such preparations can be made possible by the ÄKTA pure 25 M liquid chromatography system equipped with the proposed set of chromatography columns that include a high capacity/high resolution anion exchange column, analytical (small volume and high resolution) size exclusion columns and preparative (large volume) size exclusion columns with the latter two types targeting different molecular weights of macromolecules. The purification system and the columns will be made available to other NIH-funded investigators should the need arise.

项目总结/文摘

项目成果

Quantitative description of a contractile macromolecular machine.

• DOI: 10.1126/sciadv.abf9601
• 发表时间: 2021-06
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Fraser A;Prokhorov NS;Jiao F;Pettitt BM;Scheuring S;Leiman PG
• 通讯作者: Leiman PG

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins.

• DOI: 10.1016/j.copbio.2021.02.006
• 发表时间: 2021-04
• 期刊: Current opinion in biotechnology
• 影响因子: 7.7