National User Resource for Biological Accelerator Mass Spectrometry

国家生物加速器质谱用户资源

• 批准号: 10797225
• 负责人: Graham Bench
• 金额: $ 24.55万
• 依托单位: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797225
• 关键词: Address; Animal Model; Area; Biological; Biological Products; Biomedical Research; Biomedical Technology; C14 isotope; Cells; Code; Communities; Complex; Computer software; Data Analyses; Data Reporting; Development; Disease; Drug Kinetics; Etiology; Funding; Goals; Health; Human; Isotopes; Label; Laboratories; Lipids; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metabolic Pathway; Modification; Molecular; National Institute of General Medical Sciences; Organism; Physiological; Proteins; Radioisotopes; Research; Research Personnel; Resource Development; Resources; Sampling; Science; System; Technology; Training; United States; United States National Institutes of Health; Water; Work; accelerator mass spectrometry; base; detection limit; improved; in vivo; ion source; metabolic abnormality assessment; programs; research study; tool

PROJECT SUMMARY The National User Resource for Biological Accelerator Mass Spectrometry (User Resource) provides ultra sensitive isotopic analysis (primarily radiocarbon or Carbon-14) by accelerator mass spectrometry (AMS) for NIH-funded and other researchers. It is the only User Resource of its type in the United States. The User Resource leverages 20 years of expertise in the development and application of AMS in broad-based biomedical research through the Biomedical Technology Research Resource for the Development of Biomedical Accelerator Mass Spectrometry (BTRRAMS): a NIGMS Biomedical Technology Center (P41GM103483 09/01/2000 – 05/31/2020). Since June 2014, BTRRAMS and User Resource have measured approximately 4,000 samples a year for its user community. The User Resource will continue to meet the needs of current users as well as provide a capability for new users who would benefit from incorporating AMS as a measurement tool into their research programs. The User Resource’s forte is ultra-high sensitivity quantitation of radiocarbon and selected other radioisotopes for research studies where isotopes are required. Radioisotope labeling studies continue to be an important tool for addressing many complex problems in the biomedical sciences. AMS is a specialized and unique type of mass spectrometry that provides absolute quantitation of radiocarbon and other relevant other isotopes with limits of detection in real samples on the order of a few attograms/mg sample. As a National Resource, the most significant work enabled by AMS is likely to be quantitation of metabolic pathways in human health states and comparison to ex vivo and in vivo animal models. The technology made available enables a deeper understanding of the etiology of human health concerns by (1) enabling the ability to quantify pharmacokinetics and other molecular endpoints directly in humans; (2) offering the ability to conduct quantitative studies using biologics such as proteins or lipids; and (3) enabling more relevant studies of metabolic pathways in health and disease through the use of much lower, more biologically-relevant, concentrations of metabolic substrates in cells and intact organisms. Such studies support NIGMS’s basic biomedical research areas that contribute to the understanding of fundamental cellular and physiological principles. The User Resource has three goals: (1) provide high throughput, ultra-sensitive 14C analysis for the NIH user community; (2) improve the efficiency of ultra-sensitive 14C analysis through installation of new interfaces to our AMS systems, improvements in ion source efficiency and upgrades to our data analysis codes; and (3) increase the accessibility and visibility of ultra-sensitive 14C measurements for the biomedical research community by training new investigators and expanding the National user base of researchers requiring ultra-sensitive 14C analysis. The proposed purchase of the Waters UHPLC system will replace the no longer supported, retired Agilent 1100/1200 system in our AMS sample prep laboratory.

项目总结 生物加速器质谱学国家用户资源(用户资源)提供超 用加速器质谱仪(AMS)进行灵敏的同位素分析(主要是放射性碳或碳14) NIH资助的研究人员和其他研究人员。它是美国唯一的同类用户资源。用户 资源利用20年来在基础广泛的生物医学领域开发和应用AMS的专业知识 利用生物医药技术研究资源开发生物医药加速器 质谱仪(BTRRAMS)：NIGMS生物医学技术中心(P41GM103483 09/01/2000- 2020年5月31日)。自2014年6月以来，BTRRAMS和用户资源已经测量了大约4,000个样本 对其用户社区来说是一年。用户资源将继续满足当前用户的需求以及 为新用户提供功能，这些新用户将受益于将AMS作为测量工具整合到 研究项目。用户资源的强项是放射性碳的超高灵敏度定量和选择 其他放射性同位素，用于需要同位素的研究。放射性同位素标记研究继续进行 成为解决生物医学科学中许多复杂问题的重要工具。AMS是一种专门的 和独特类型的质谱学，提供放射性碳和其他相关物质的绝对定量 在实际样品中的检出限为几个微克/毫克样品的其他同位素。作为一名国民 资源，AMS实现的最重要的工作可能是量化人类的代谢途径 健康状况以及与体外和体内动物模型的比较。可用的技术使一种 通过以下方式更深入地理解人类健康问题的病因学：(1)能够量化 直接在人体内的药代动力学和其他分子终点；(2)提供进行定量 使用蛋白质或脂类等生物制剂的研究；以及(3)能够对代谢途径进行更相关的研究 在健康和疾病中，通过使用更低的、更与生物相关的代谢浓度 细胞和完整有机体中的底物。这些研究支持NIGMS的基础生物医学研究领域 有助于理解基本的细胞和生理原理。用户资源具有 三个目标：(1)为NIH用户社区提供高通量、超灵敏的14C分析；(2)提高 通过为我们的AMS系统安装新接口来提高超灵敏14C分析的效率，改进 提高离子源效率并升级我们的数据分析代码；以及(3)提高可访问性和可见性 通过培训新的研究人员和为生物医学研究社区提供超灵敏14C测量 扩大需要超灵敏14C分析的研究人员的全国用户基础。拟议中的收购 将取代我们AMS中不再支持的、已停用的安捷伦1100/1200系统 样品预备室。

项目成果

Development of a CNS-permeable reactivator for nerve agent exposure: an iterative, multi-disciplinary approach.

• DOI: 10.1038/s41598-021-94963-2
• 发表时间: 2021-07-30
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Bennion BJ;Malfatti MA;Be NA;Enright HA;Hok S;Cadieux CL;Carpenter TS;Lao V;Kuhn EA;McNerney MW;Lightstone FC;Nguyen TH;Valdez CA
• 通讯作者: Valdez CA

Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14-Labeled Analytes.

碳 14 标记分析物的并行加速器和分子质谱测量。

• DOI: 10.1007/978-1-0716-1585-0_1
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Stewart,BenjaminJ;Ognibene,TedJ
• 通讯作者: Ognibene,TedJ

Distribution and metabolism of [14C]-resveratrol in human prostate tissue after oral administration of a "dietary-achievable" or "pharmacological" dose: what are the implications for anticancer activity?

• DOI: 10.1093/ajcn/nqaa414
• 发表时间: 2021-05-08
• 期刊: The American journal of clinical nutrition
• 作者: Cai H;Scott EN;Britton RG;Parrott E;Ognibene TJ;Malfatti M;Khan M;Steward WP;Brown K
• 通讯作者: Brown K

Disentangling the History of Deep Ocean Disposal for DDT and Other Industrial Waste Off Southern California.

解开南加州近海深海处置 DDT 和其他工业废物的历史。

• DOI: 10.1021/acs.est.3c08575
• 发表时间: 2024
• 期刊: Environmental science & technology
• 影响因子: 11.4
• 作者: Schmidt,JacobT;Wu,MongSinChristine;Kittner,HailieE;Arey,JSamuel;Hammond,DouglasE;Group,EarthA;Valentine,DavidL
• 通讯作者: Valentine,DavidL