Receptor kinase signal integration in stem cell maintenance and development.

干细胞维持和发育中的受体激酶信号整合。

• 批准号: 10797759
• 负责人: Zachary Luke Nimchuk
• 金额: $ 3.85万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-04 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797759
• 关键词: Animals; Cell Maintenance; Desiccation; Development; Dryness; Equipment; Eukaryota; Funding; Genes; Image; Peptide Signal Sequences; Peptides; Phosphotransferases; Plant Preparations; Plant Stems; Plants; Play; Process; Regulation; Role; Scanning Electron Microscopy; Signal Transduction; System; Tissue Sample; Tissues; instrumentation; mutant; plant growth/development; receptor; stem cell niche; stem cells

The project is requesting funds for a Leica EM CPD300 automated Critical Point Drying system for preparing plant tissue samples for Scanning Electron Microscopy. This proposal supports R35GM119614 which aims to understand the role of CLE peptide signaling in stem cell regulation and development in plants. SEM has been instrumental in ultrastructural analysis of plant tissues in this project, however the lack of critical point drying equipment has limited the scope of tissue types that can be analyzed. As such Leica EM CPD300 will enable the imaging of delicate and easily desiccated plant tissues rapidly and in a high throughput manner and will assist the analysis of the processes CLE peptides play in plant development.

该项目正在申请Leica EM CPD 300自动临界点干燥的资金 用于制备扫描电子显微镜用植物组织样品的系统。这项建议 支持R35GM119614，旨在了解CLE肽信号传导在干细胞中的作用 植物的生长和发育。扫描电镜已在超微结构分析的工具， 本项目中的植物组织，但缺乏临界点干燥设备，限制了 可以分析的组织类型的范围。因此，Leica EM CPD 300将使成像 以高通量的方式快速地提取脆弱且容易干燥的植物组织， 有助于分析CLE肽在植物发育中的作用。

项目成果

A conserved module regulates receptor kinase signalling in immunity and development

• DOI: 10.1038/s41477-022-01134-w
• 发表时间: 2022-04-14
• 期刊: NATURE PLANTS
• 影响因子: 18
• 作者: DeFalco, Thomas A.;Anne, Pauline;Nimchuk, Zachary L.
• 通讯作者: Nimchuk, Zachary L.

Evolution of buffering in a genetic circuit controlling plant stem cell proliferation

• DOI: 10.1038/s41588-019-0389-8
• 发表时间: 2019-05-01
• 期刊: NATURE GENETICS
• 影响因子: 30.8
• 作者: Rodriguez-Leal, Daniel;Xu, Cao;Lippman, Zachary B.
• 通讯作者: Lippman, Zachary B.