Spatial Organization and Regulation of Bacterial Cellular Processes

细菌细胞过程的空间组织和调节

• 批准号: 10799126
• 负责人: Jie Xiao
• 金额: $ 13.11万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799126
• 关键词: Antibiotics; Bacterial Infections; Bacteriophages; Biochemical; Biological Models; Cell Wall; Cell division; Cell physiology; Cells; Cellular biology; Communicable Diseases; Complex; Cytolysis; Cytoplasm; DNA; DNA-Directed RNA Polymerase; Development; Escherichia coli; Genetic Transcription; Genome; Goals; Knowledge; Laboratories; Molecular; Positioning Attribute; Process; Proteins; Regulation; Research; Work; combat; insight; interest; model organism; molecular imaging; pathogenic bacteria; single molecule; transcription factor; treadmill

Project Summary Bacterial cells, despite their relatively small genomes and non-compartmentalized cytoplasm, perform a variety of complex cellular tasks that are regulated both at the molecular level through canonical biochemical interactions and at the subcellular level through the spatial organization of molecules. My laboratory is broadly interested in understanding how the molecular constituents of bacterial cellular processes are spatially organized and what essential functions such an organization conveys. Our current research focuses on bacterial cell division, transcription and phage-host interactions using E. coli as a model organism. Specifically, we will (1) determine the spatial coordination and regulation of the septal cell wall synthase complex FtsWI by FtsZ’s treadmilling dynamics; (2) investigate the spatial organizations of RNA polymerase, essential transcription factors and chromosomal DNAs and their associated functions; and (3) probe the localization, dynamics and interactions of phage lysis proteins in cell’s envelop. We are uniquely positioned to take on these tasks because of our extensive expertise in quantitative single molecule imaging and bacterial cell biology. We expect our work to provide new molecular insight that could be used to develop new strategies to treat bacterial infection.

项目摘要 细菌细胞，尽管它们的基因组相对较小，细胞质不分区， 在分子水平上通过典型的生物化学相互作用 在亚细胞水平上通过分子的空间组织。我的实验室对 了解细菌细胞过程的分子组成是如何在空间上组织的， 这样一个组织的基本功能。我们目前的研究重点是细菌细胞分裂， 转录和噬菌体-宿主相互作用。大肠杆菌作为模式生物。具体而言，我们将（1）确定 通过FtsZ’s旋转铣削对隔细胞壁合酶复合物FtsWI的空间协调和调节 （2）研究RNA聚合酶、必需转录因子和 染色体DNA及其相关功能;和（3）探测的定位，动力学和相互作用， 噬菌体溶解细胞包膜中的蛋白质。我们在承担这些任务方面处于独特的地位，因为我们拥有广泛的 在定量单分子成像和细菌细胞生物学方面的专业知识。我们希望我们的工作能提供新的 分子洞察力，可用于开发新的策略来治疗细菌感染。