High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues

多种矿化组织内细胞异质性的高分辨率 3D 绘图

• 批准号: 10816791
• 负责人: Martin K Lotz
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10816791
• 关键词: 3-Dimensional; Antibodies; Antigen Targeting; Binding; Biological Process; Cells; Chondrocytes; Collaborations; DNA; Data; Data Set; Databases; Degenerative polyarthritis; Detection; Development; Fluorescent Probes; Gene Combinations; Gene Expression; Gene Targeting; Genes; Genetic Transcription; Group Processes; Health; Heterogeneity; Histologic; Human; Image; Imaging Techniques; Individual; Knee; Label; Link; Location; Maps; Methods; Molecular; Monoclonal Antibodies; Names; Organelles; Pattern; Process; Property; Proteins; RNA; Reaction; Recombinants; Research; Resolution; Sampling; Scanning; Signal Transduction; Single-Stranded DNA; Sister; Source; Spatial Distribution; Stress; Surface; Synovial joint; Technology; Tissues; Visual; Work; antibody detection; articular cartilage; cartilage cell; cartilage degradation; cell type; combinatorial; design; detection method; functional group; histological image; land use; mineralization; multimodality; parent grant; skeletal tissue; skills; technology development; tool; virtual

Abstract The parent grant is a collaboration of two complementary research groups that is designed to spatially map the chondrocytes that form and maintain the human articular cartilage (AC) of a synovial joint which in this case is the knee. The Scripps component obtains and processes the experimental samples for detailed molecular expression studies. The UCONN component utilizes its skills in histological imaging of skeletal tissues to provide the spatial distribution of the cells that are identified by the molecular expression data using an RNA identification method called MERFISH. The activities proposed in this supplement expands the identification technology to utilize antibody binding to the proteins encoded by the genes (CODEX). The supplement will support the implementation of this antibody detection method to multiple targets so that groups of cells with a defined biological function can be identified and their location mapped to specific zone of the human AC. The two imaging techniques should be complementary and provide a high degree of confidence in patterns of distribution of functional groups of cells within the 3 zone of the AC. Eventually it will serve as the baseline for appreciating a disturbance in the patterns when the tissue is under a stress that can lead to the early stages of cartilage degeneration and the eventual onset of osteoarthritis.

抽象的 家长资助是两个互补研究小组的合作，旨在 对形成和维持滑膜的人类关节软骨 (AC) 的软骨细胞进行空间映射 关节，在这种情况下是膝盖。 Scripps 组件获取并处理实验数据 用于详细分子表达研究的样品。 UCONN 组件利用其技能 骨骼组织的组织学成像，以提供所识别细胞的空间分布 通过使用称为 MERFISH 的 RNA 识别方法获得分子表达数据。活动内容 本补充中提出的扩展了识别技术，以利用抗体与 由基因编码的蛋白质（CODEX）。该补充文件将支持本规定的实施 针对多个目标的抗体检测方法，使细胞群具有明确的生物学功能 可以被识别并将其位置映射到人类 AC 的特定区域。两种成像 技术应该是互补的，并提供对模式的高度信心 AC 3 区内细胞功能群的分布。最终它将作为 当组织处于可能导致 软骨退化的早期阶段和骨关节炎的最终发作。

项目成果

vSPACE: Exploring Virtual Spatial Representation of Articular Chondrocytes at the Single-Cell Level.

vSPACE：探索单细胞水平的关节软骨细胞的虚拟空间表示。

• DOI: 10.1101/2024.02.07.577817
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Zhang,Chenyu;Wang,Honglin;Hong,Seung-Hyun;Olmer,Merissa;Swahn,Hannah;Lotz,MartinK;Maye,Peter;Rowe,David;Shin,Dong-Guk
• 通讯作者: Shin,Dong-Guk

rPAC: Route based pathway analysis for cohorts of gene expression data sets.

• DOI: 10.1016/j.ymeth.2021.10.002
• 发表时间: 2022-03
• 期刊: Methods (San Diego, Calif.)
• 作者: Joshi P;Basso B;Wang H;Hong SH;Giardina C;Shin DG
• 通讯作者: Shin DG