High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues

多种矿化组织内细胞异质性的高分辨率 3D 绘图

• 批准号: 10816791
• 负责人: Martin K Lotz
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10816791
• 关键词: 3-Dimensional; Antibodies; Antigen Targeting; Binding; Biological Process; Cells; Chondrocytes; Collaborations; DNA; Data; Data Set; Databases; Degenerative polyarthritis; Detection; Development; Fluorescent Probes; Gene Combinations; Gene Expression; Gene Targeting; Genes; Genetic Transcription; Group Processes; Health; Heterogeneity; Histologic; Human; Image; Imaging Techniques; Individual; Knee; Label; Link; Location; Maps; Methods; Molecular; Monoclonal Antibodies; Names; Organelles; Pattern; Process; Property; Proteins; RNA; Reaction; Recombinants; Research; Resolution; Sampling; Scanning; Signal Transduction; Single-Stranded DNA; Sister; Source; Spatial Distribution; Stress; Surface; Synovial joint; Technology; Tissues; Visual; Work; antibody detection; articular cartilage; cartilage cell; cartilage degradation; cell type; combinatorial; design; detection method; functional group; histological image; land use; mineralization; multimodality; parent grant; skeletal tissue; skills; technology development; tool; virtual

Abstract The parent grant is a collaboration of two complementary research groups that is designed to spatially map the chondrocytes that form and maintain the human articular cartilage (AC) of a synovial joint which in this case is the knee. The Scripps component obtains and processes the experimental samples for detailed molecular expression studies. The UCONN component utilizes its skills in histological imaging of skeletal tissues to provide the spatial distribution of the cells that are identified by the molecular expression data using an RNA identification method called MERFISH. The activities proposed in this supplement expands the identification technology to utilize antibody binding to the proteins encoded by the genes (CODEX). The supplement will support the implementation of this antibody detection method to multiple targets so that groups of cells with a defined biological function can be identified and their location mapped to specific zone of the human AC. The two imaging techniques should be complementary and provide a high degree of confidence in patterns of distribution of functional groups of cells within the 3 zone of the AC. Eventually it will serve as the baseline for appreciating a disturbance in the patterns when the tissue is under a stress that can lead to the early stages of cartilage degeneration and the eventual onset of osteoarthritis.

摘要

项目成果

vSPACE: Exploring Virtual Spatial Representation of Articular Chondrocytes at the Single-Cell Level.

vSPACE：探索单细胞水平的关节软骨细胞的虚拟空间表示。

• DOI: 10.1101/2024.02.07.577817
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Zhang,Chenyu;Wang,Honglin;Hong,Seung-Hyun;Olmer,Merissa;Swahn,Hannah;Lotz,MartinK;Maye,Peter;Rowe,David;Shin,Dong-Guk
• 通讯作者: Shin,Dong-Guk

rPAC: Route based pathway analysis for cohorts of gene expression data sets.

• DOI: 10.1016/j.ymeth.2021.10.002
• 发表时间: 2022-03
• 期刊: Methods (San Diego, Calif.)
• 作者: Joshi P;Basso B;Wang H;Hong SH;Giardina C;Shin DG
• 通讯作者: Shin DG