High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues

摘要 家长资助是两个互补的研究小组的合作，旨在 对形成和维持人类滑膜关节软骨的软骨细胞进行空间定位 关节，在这种情况下是膝盖。Scripps组件获取并处理实验数据 用于详细的分子表达研究的样本。康涅狄格州大学的组件在 骨骼组织的组织成像，以提供所识别的细胞的空间分布 由分子表达数据使用一种名为MerFish的RNA鉴定方法。活动 在本补编中建议扩展识别技术，以利用与 由基因编码的蛋白质(CODEX)。本补编将支持这项工作的实施。 一种对多个靶点进行抗体检测的方法 可以被识别并将它们的位置映射到人类AC的特定区域。两种成像 技术应该是互补的，并在模式中提供高度的置信度 AC 3区内细胞功能群的分布。最终，它将成为 当组织处于可能导致的压力下时，判断图案中的干扰的基线 软骨退化的早期阶段和骨性关节炎的最终发病。

Abstract The parent grant is a collaboration of two complementary research groups that is designed to spatially map the chondrocytes that form and maintain the human articular cartilage (AC) of a synovial joint which in this case is the knee. The Scripps component obtains and processes the experimental samples for detailed molecular expression studies. The UCONN component utilizes its skills in histological imaging of skeletal tissues to provide the spatial distribution of the cells that are identified by the molecular expression data using an RNA identification method called MERFISH. The activities proposed in this supplement expands the identification technology to utilize antibody binding to the proteins encoded by the genes (CODEX). The supplement will support the implementation of this antibody detection method to multiple targets so that groups of cells with a defined biological function can be identified and their location mapped to specific zone of the human AC. The two imaging techniques should be complementary and provide a high degree of confidence in patterns of distribution of functional groups of cells within the 3 zone of the AC. Eventually it will serve as the baseline for appreciating a disturbance in the patterns when the tissue is under a stress that can lead to the early stages of cartilage degeneration and the eventual onset of osteoarthritis.