Mapping the joint-nerve interactome of the knee
绘制膝关节的关节神经相互作用组图
基本信息
- 批准号:10861323
- 负责人:
- 金额:$ 122.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-13 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:ATAC-seqAddressAgeAntibodiesAtlasesBilateralBlood VesselsCellsChronic low back painConnective TissueDegenerative polyarthritisDiseaseFacet joint structureFemaleFundingGene Expression ProfileGenerationsGenetic TranscriptionGoalsHumanIntervertebral disc structureJointsKneeKnee OsteoarthritisKnee jointLow Back PainMapsMethodsMolecularMolecular AnalysisMuscleNerveNeuronsNociceptorsPainParentsPathologicPhasePopulationPriceProprioceptorProteinsResearchResearch PersonnelResolutionSensorySmall Nuclear RNASpatial DistributionSpinal GangliaStructureTemporomandibular JointTexasTissue BanksTissue DonorsTissuesUniversitiesVertebral columnWorkagedaxon guidancecell typechronic painepigenomegene regulatory networkhuman old age (65+)insightintervertebral disk degenerationknee painmalemouse modelnerve supplyneuralneurotrophic factornovelparent grantprogramstherapeutic targetthree-dimensional modelingtissue degenerationtranscriptometranscriptome sequencingtranscriptomicsvertebra body
项目摘要
ABSTRACT
This supplement addresses are proposed studies are in the context of spine tissue damage and chronic low
back pain (LBP). The goals of the parent grant are to construct 3D models of the sensory innervation of the knee,
compose a cell atlas in which knee afferents are transcriptionally profiled at a single cell resolution, and document
the nerve-joint cell interactome at the transcriptional level.
The supplement substantially expands the scope and potential impact of the parent program by examining
mechanisms of intervertebral disc (IVD) degeneration (IDD), facet joint (FJ) osteoarthritis (OA) and the
interaction of these tissues with neurons in the generation of low back pain. The inclusion of the FJ also extends
the portfolio of joints (knee and temporomandibular joints) that are currently being investigated in the RE-JOIN
consortium.
The team includes investigators at Rush University (PI Dr. Anne-Marie Malfait) and Scripps Research (PI Dr.
Martin Lotz) in the funded UC2 project for the analysis of knee joints and adds new methods for the analysis of
IVD and FC degeneration and the team at the University of Texas, Dallas (PI Dr. Theodore Price) adds expertise
in the analysis of dorsal root ganglia (DRG). The team at Scripps has a long-standing program on mechanisms
of knee OA and performed prior work on mechanisms of IDD, using mouse models and human spines. Omics
analyses were applied to define cell populations and their gene regulatory networks in healthy and degenerated
IVD. The team at Rush is a leader in the analysis of pain mechanisms in OA and has expertise in the analysis
of neural structures in knee joints and in the analysis of chronic pain mechanisms. The two teams are working
together in the funded RE-JOIN project about knee pain. Our unique approach is to examine spine and knee
tissues from the same young healthy and older donors with OA and IDD for innervation, transcriptome and
epigenome at tissue, single cell, and spatial levels. The integrative analysis of neural and connective tissue
changes during IDD will reveal critical interactions that promote tissue damage and pain.
摘要
本补充地址是建议的研究是在脊柱组织损伤和慢性低
背痛(LBP)。父母资助的目标是构建膝关节感觉神经支配的3D模型,
组成一个细胞图谱,其中膝传入神经以单细胞分辨率进行转录分析,并记录
神经-关节细胞相互作用组在转录水平。
该补充大大扩大了范围和潜在影响的母计划,通过审查
椎间盘(IVD)退变(IDD)、小关节(FJ)骨关节炎(OA)和
这些组织与神经元的相互作用在腰痛的产生。FJ的加入也扩大了
目前正在RE-JOIN中研究的关节组合(膝关节和颞下颌关节)
财团
该小组包括拉什大学(PI博士安妮玛丽Malfait)和斯克里普斯研究(PI博士。
Martin Lotz)在用于膝关节分析的UC 2资助项目中,
IVD和FC变性和团队在得克萨斯大学,达拉斯(PI博士西奥多价格)增加专业知识
背根神经节(DRG)的分析。斯克里普斯的团队有一个长期的机制计划
的膝关节OA和IDD的机制进行了前期工作,使用小鼠模型和人类脊柱。组学
分析应用于定义健康和退化的细胞群体及其基因调控网络,
IVD。Rush的团队是OA疼痛机制分析的领导者,并具有分析方面的专业知识
在膝关节神经结构和慢性疼痛机制的分析。两支队伍正在努力
一起参加了一个关于膝盖疼痛的RE-JOIN项目。我们独特的方法是检查脊柱和膝盖
来自相同的年轻健康和患有OA和IDD的老年供体的组织用于神经支配、转录组和
表观基因组在组织、单细胞和空间水平。神经与结缔组织的综合分析
IDD期间的变化将揭示促进组织损伤和疼痛的关键相互作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Martin K Lotz其他文献
New approach to testing treatments for osteoarthritis: FastOA
骨关节炎治疗测试新方法:快速骨关节炎评估法
- DOI:
10.1136/ard-2023-224675 - 发表时间:
2024-03-01 - 期刊:
- 影响因子:20.600
- 作者:
David Felson;Martin K Lotz;Yuxuan Jin;Morgan Jones;Jason S Kim;Kurt Spindler - 通讯作者:
Kurt Spindler
Martin K Lotz的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Martin K Lotz', 18)}}的其他基金
Mapping the joint-nerve interactome of the knee
绘制膝关节的关节神经相互作用组图
- 批准号:
10607479 - 财政年份:2022
- 资助金额:
$ 122.35万 - 项目类别:
High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues
多种矿化组织内细胞异质性的高分辨率 3D 绘图
- 批准号:
10705190 - 财政年份:2020
- 资助金额:
$ 122.35万 - 项目类别:
High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues
多种矿化组织内细胞异质性的高分辨率 3D 绘图
- 批准号:
10267740 - 财政年份:2020
- 资助金额:
$ 122.35万 - 项目类别:
High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues
多种矿化组织内细胞异质性的高分辨率 3D 绘图
- 批准号:
10700252 - 财政年份:2020
- 资助金额:
$ 122.35万 - 项目类别:
High resolution 3D mapping of cellular heterogeneity within multiple types of mineralized tissues
多种矿化组织内细胞异质性的高分辨率 3D 绘图
- 批准号:
10816791 - 财政年份:2020
- 资助金额:
$ 122.35万 - 项目类别:
FOXO transcription factors as critical regulators of intervertebral disc aging
FOXO转录因子作为椎间盘老化的关键调节因子
- 批准号:
10617735 - 财政年份:2019
- 资助金额:
$ 122.35万 - 项目类别:
FOXO transcription factors as critical regulators of intervertebral disc aging
FOXO转录因子作为椎间盘老化的关键调节因子
- 批准号:
10399475 - 财政年份:2019
- 资助金额:
$ 122.35万 - 项目类别:
FoxO transcription factors in joint aging and osteoarthritis pathogenesis
FoxO转录因子在关节衰老和骨关节炎发病机制中的作用
- 批准号:
10399471 - 财政年份:2018
- 资助金额:
$ 122.35万 - 项目类别:
KLF4 in joint degradation and regeneration
KLF4 在关节降解和再生中的作用
- 批准号:
9927548 - 财政年份:2018
- 资助金额:
$ 122.35万 - 项目类别:
FoxO transcription factors in joint aging and osteoarthritis pathogenesis
FoxO转录因子在关节衰老和骨关节炎发病机制中的作用
- 批准号:
9916681 - 财政年份:2018
- 资助金额:
$ 122.35万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 122.35万 - 项目类别:
Research Grant