Cytoskeletal Function in C. elegans Embryos

项目摘要 Alyssa将使用分子遗传学和高分辨率光学显微镜进一步推进她 发现微管是线虫卵母细胞皮质的组成部分，其中 它们的作用是使皮质变硬，从而限制膜的侵入范围。 卵母细胞减数分裂和极体排出。通过额外的培训，Alyssa将 获得广泛的分子遗传学新技能(光遗传学、基因替换和 基因组编辑)和显微镜(接近TIRF、Zeiss AiryScan和尼康旋转磁盘SORA 显微镜)。她还将在协作、跨学科研究方面获得丰富的经验 我们的成像设备Adam Fries博士和我实验室的高级研究助理，以及 她将在论文工作期间指导本科生。Alyssa还将更多地关注 建立联系，特别是在接下来的两年里参加多个科学会议。 最后，Alyssa将获得广泛的写作和演讲经验，因为这些是 她最需要改进的领域。通过将她所有的时间都投入到实验室工作中， 不需要教书，因为我现在的米拉剩余一年的预算不足 资金，Alyssa将更有可能凭借多份出版物和在 写作和口语将极大地提高她作为独立人士在职业生涯中取得成功的能力 调查员。

Project Summary Alyssa will use molecular genetics and high resolution light microscopy to further advance her discovery that microtubules are an integral component of the C. elegans oocyte cortex, where they serve to stiffen the cortex and thereby limit the extent of membrane ingressions during oocyte meiotic cell division and polar body extrusion. Through additional training, Alyssa will acquire extensive new skills in molecular genetics (optogenetics, gene replacement and genome editing) and microscopy (near TIRF, Zeiss Airyscan, and Nikon Spinning Disk SoRa microscopy). She also will gain extensive experience in collaborative, interdisciplinary research with our Imaging Facility Dr. Adam Fries and senior research associates in my lab, and with the undergraduates that she will mentor during her thesis work. Alyssa also will focus more on networking, particularly by attending multiple science conferences over the next two years. Finally, Alyssa will gain extensive additional writing and speaking experience, as these are the areas in which she most needs improvement. By being able to devote all of her time to lab work, without having to teach due to my insufficient budget for the remaining year of my current MIRA funding, Alyssa will be far more likely to excel with multiple publications and improvements in writing and speaking that will greatly improve her ability to succeed in career as an independent investigator.