Molecular and serological surveillance of SARS-CoV-2 in companion and food animals, USA

项目摘要/摘要 伴侣和食物中SARS-CoV-2的分子和血清学监测 animals，美国 监测动物中的SARS-CoV-2感染对于更好地了解 病毒及其在物种间传播的可能性。病毒已经 被证明感染了一系列动物，包括水貂、狗和猫。通过监测动物 感染，可以确定病毒的潜在储存库，并可能 可以防止更易传染或更致命的疾病。此外，监测动物感染 通过识别和控制，确保动物和人类群体的安全 动物感染。 在本研究中，我们将使用PCR和ELISA检测SARS-CoV-2病毒， 在全国范围内收集的犬样本（n=800）、猫样本（n=800）和 来自亚拉巴马的马（n=200）和牛（n=200）样品。SARS-CoV-2逆转录病毒- 将进行转录FRET-PCR检测病毒，然后进行DNA 测序和全基因组测序。ID Screen® SARS-CoV-2 Double 将使用抗原ELISA和SARS-CoV-2替代病毒中和试验（sVNT） 检测SARS-CoV-2感染的抗体，阳性和可疑 样品将被送往美国农业部（USDA）国家 兽医服务实验室（NVSL）进行SARS-CoV-2病毒中和测试， 确认结果。 监测动物群体的SARS-CoV-2感染对于预防 病毒向人类的传播，并确定病毒的潜在宿主。本研究 也将有助于了解SARS-CoV-2在狗、猫和马中的流行病学， 以及美国的食用动物。

Project Abstract/Summary Molecular and serological surveillance of SARS-CoV-2 in companion and food animals, USA Monitoring SARS-CoV-2 infections in animals is crucial for better understanding the virus and its potential for transmission between species. The virus has already been shown to infect a range of animals, including minks, dogs, and cats. By monitoring animal infections, potential reservoirs of the virus can be identified and new strains that may be more transmissible or virulent can be prevented. Additionally, monitoring animal infections ensures the safety of both animal and human populations by identifying and controlling infections in animals. In this study, we will perform PCR and ELISAs to detect SARS-CoV-2 virus and antibodies in nationwide collected dog samples (n=800), cat samples (n=800), and in horse (n=200) and cattle (n=200) samples from Alabama. SARS-CoV-2 Reverse- Transcription FRET-PCR will be performed to detect the virus, followed by DNA sequencing and whole-genome sequencing. The ID Screen® SARS-CoV-2 Double Antigen ELISA and SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) will be used to detect antibodies against SARS-CoV-2 infection, and positive and questionable samples will be sent to the United States Department of Agriculture (USDA) National Veterinary Services Laboratories (NVSL) for SARS-CoV-2 virus neutralization tests to confirm results. Monitoring animal populations for SARS-CoV-2 infections is essential to prevent the spread of the virus to humans and identify potential reservoirs of the virus. This study will also help to understand the epidemiology of SARS-CoV-2 in dogs, cats, and horses, as well as food animals in the USA.