Occurrence of antimicrobial-resistant bacteria from client-owned pets and their raw meat-based diets

客户拥有的宠物及其生肉饮食中出现抗菌药物耐药性细菌

• 批准号: 10213897
• 负责人: Chengming Wang
• 金额: $ 3.73万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-20 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10213897
• 关键词: Occurrence; antimicrobial; resistant; bacteria; client

Project Abstract/Summary Occurrence of antimicrobial-resistant bacteria from client-owned pets and their raw meat-based diets Advocates of raw‐meat diets claim various health benefits for dogs, and while none of the purported benefits have been validated, owners of raw meat‐fed animals stand by their convictions (1-4). In contrast, a 2-year study conducted by the US Food and Drug Administration found that raw pet food is more likely than other types of pet food to carry bacteria, including Salmonella and Listeria monocytogenes (5). The FDA issued a public health warning about the risks of raw pet food diets and both the American Animal Hospital Association and the American Veterinary Medical Association officially recommend against feeding raw meat-based diets to dogs. Numerous studies have validated the concerns associated with raw food diets (RFD), but none has determined the prevalence of drug resistant bacteria (6-8). To address this question, fecal specimens will be collected from animals fed a RFD. Samples will also be collected from animals on a commercial diet, but also receiving antibiotics and others considered antibiotic naïve. The survey focus will be on multidrug resistant bacteria including vancomycin-resistant enterococci (VRE), carbapenemase-producing bacteria and colistin resistant Enterobacteriaceae, but random enteric isolates will be selected for testing for antimicrobial resistance. Fecal samples will be submitted in Cary-Blair medium and inoculated onto imipenem containing MacConkey agar to select for carbapenem-resistant bacteria. Lucie Bardet- Jean Marc Rolain media will be utilized to select for colistin-resistant bacteria and VRE (9). Conventional biochemical testing including the Vitek 2 Gram-positive and Gram- negative ID cards (BioMerieux) will be utilized for bacterial identifications (10). Antimicrobial susceptibility profiles will be determined by micro broth dilution (Sensititre® automated system; Trek Diagnostics, Westlake, OH, USA) and will include antimicrobials utilized in the National Antimicrobial Monitoring System. Isolates that are ESBL positive will undergo multiplex qPCR for the extended spectrum beta lactamases blaTEM, blaSHV, blaCTX-M and blaCMY-2 and sequencing to determine the variant (11, 12). Multiplex qPCR platforms will be utilized to examine for plasmid mediated colistin resistance genes: mcr-1 to-mcr-9 and tet (M), tet (L), cat, cmlA which confer resistance to the tetracyclines and phenicols (13, 14).

项目摘要/总结 客户拥有的宠物及其宠物中出现抗菌药物耐药性细菌 以生肉为主的饮食 生肉饮食的倡导者声称对狗有多种健康益处，但没有一个 声称的好处已得到验证，生肉喂养动物的主人对此表示支持 他们的信念（1-4）。相比之下，美国食品和药物管理局进行的一项为期 2 年的研究 主管部门发现，生宠物食品比其他类型的宠物食品更有可能携带 细菌，包括沙门氏菌和单核细胞增生李斯特氏菌 (5)。 FDA 发布了 关于生宠物食品饮食风险的公共卫生警告以及美国 动物医院协会与美国兽医协会正式合作 建议不要给狗喂生肉为主的饮食。 大量研究证实了与生食饮食 (RFD) 相关的担忧， 但尚未确定耐药细菌的流行程度 (6-8)。致地址 对于这个问题，粪便样本将从饲喂 RFD 的动物身上采集。样品将 也可以从商业饮食的动物身上收集，但也接受抗生素和 其他人则认为没有使用抗生素。调查重点将是多重耐药性 细菌，包括耐万古霉素肠球菌 (VRE)、产碳青霉烯酶 细菌和粘菌素耐药肠杆菌科细菌，但随机肠道分离株将 选择用于测试抗菌药物耐药性。 粪便样本将在 Cary-Blair 培养基中提交并接种到亚胺培南上 含有麦康凯琼脂以选择碳青霉烯类耐药细菌。露西·巴代- Jean Marc Rolain 培养基将用于选择粘菌素耐药细菌和 VRE （9）。常规生化检测包括 Vitek 2 革兰氏阳性和革兰氏阳性 阴性 ID 卡 (BioMerieux) 将用于细菌鉴定 (10)。 抗菌药物敏感性曲线将通过微量肉汤稀释（Sensititre® 自动化系统； Trek Diagnostics（美国俄亥俄州西湖），并将包括 国家抗菌药物监测系统中使用的抗菌药物。分离物是 ESBL 阳性将接受多重 qPCR 检测广谱 β 内酰胺酶 blaTEM、blaSHV、blaCTX-M 和 blaCMY-2 以及测序以确定变异 (11, 12)。多重 qPCR 平台将用于检查质粒介导的 粘菌素抗性基因：mcr-1 至 mcr-9 和 tet (M)、tet (L)、cat、cmlA，这些基因赋予 对四环素和苯酚的耐药性 (13, 14)。