Antimicrobial Resistance Monitoring of Bacterial Pathogens of Veterinary Concern

兽医关注的细菌病原体的抗菌药物耐药性监测

• 批准号: 10829130
• 负责人: Claire R Burbick
• 金额: $ 7.5万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10829130
• 关键词: Antimicrobial; Resistance; Monitoring; Bacterial; Pathogens

1 Project Summary: Resistant bacterial infections impact animal health and welfare, but they also 2 have potential for causing negative human health consequences through transmission of resistant 3 bacteria or resistance genes through food or animal contact. The use of common classes of 4 antimicrobials in humans and animals increases the likelihood that drug resistance selected for in 5 animal species could impact humans and vice versa. Therefore, monitoring AMR in animals has 6 the potential to contribute to mitigation not only disease in animals but human infections as well. 7 Coordinated nationwide AMR monitoring in veterinary species for bacteria of importance in 8 major sectors of veterinary medicine is has been a recent phenomenon, and many data gaps exist. 9 Veterinary diagnostic laboratories are uniquely positioned to contribute to antimicrobial 10 resistance (AMR) monitoring through access to clinically relevant bacterial isolates and technical 11 expertise in laboratory testing. This project addresses these gaps by contributing to standardized 12 method development for whole genome sequencing to ensure the sensitivity and specificity is 13 appropriate for detecting the resistance of interest, improvement of the bioinformatics pipeline 14 with quality assurance and quality control criteria tailored to the veterinary diagnostic laboratory 15 and provide AMR data in agricultural and companion animal species. Each year of funding will 16 involve coordination with veterinary diagnostic source laboratories for acquisition of bacterial 17 isolates derived from clinically affected animals, including Salmonella, E. coli and 18 Staphylococcus pseudintermedius and other bacteria of interest, from across the US, performance 19 of whole genome sequencing of on a quarterly basis, and evaluation for the presence of 20 antimicrobial resistance genes. The data will be provided to FDA CVM Vet-LIRN for further 21 evaluation and future public availability. 22 23

耐药细菌感染影响动物健康和福利，但它们也 2.有可能通过耐药菌的传播对人类健康造成负面影响 3细菌或耐药基因通过食物或动物接触。使用通用类 人类和动物中的4种抗生素增加了耐药性选择的可能性， 5种动物可能影响人类，反之亦然。因此，在动物中监测AMR 6.不仅有助于减轻动物疾病，而且有助于减轻人类感染的潜力。 7.在全国范围内协调监测兽医种属中重要细菌的AMR， 8大兽医行业是最近才出现的现象，存在许多数据空白。 9个兽医诊断实验室具有独特的优势，可为抗菌药物 通过获得临床相关的细菌分离株和技术支持进行耐药性（AMR）监测 11实验室测试专业知识该项目通过促进标准化， 12全基因组测序的方法开发，以确保灵敏度和特异性， 13适合于检测感兴趣的抗性，改进生物信息学管道 14个兽医诊断实验室的质量保证和质量控制标准 15并提供农业和伴侣动物物种的AMR数据。每年的资金将 16涉及与兽医诊断源实验室协调，以获得细菌 17株来自临床感染动物，包括沙门氏菌、E.杆菌和 18假中间葡萄球菌和其他感兴趣的细菌，来自美国各地，性能 每季度进行19次全基因组测序， 20个抗菌素耐药基因。数据将提供给FDA CVM Vet-LIRN，以进一步 21评价和今后的公开提供。 22 23