Monitoring antimicrobial resistance among veterinary pathogens at Iowa State University Veterinary Diagnostic Laboratory: A Vet-LIRN AMR Surveillance Project

项目总结/摘要 监测爱荷华州州立大学兽医病原体的耐药性 兽医诊断实验室：兽医-LIRN AMR监测项目 抗菌素耐药性（AMR）是影响人类健康的主要健康威胁， 动物和世界各地的环境。在美国，兽医实验室 美国食品和药物管理局下属的调查和反应网络（Vet-LIRN）， 兽医中心（FDA-CVM）在选定的兽医中进行AMR监测 细菌病原体（例如，沙门氏菌、大肠杆菌和假中间葡萄球菌） 通过与北美各地的诊断实验室合作，下 倡议，爱荷华州州立大学兽医诊断实验室（ISU VDL）成为兽医， LIRN源实验室，并已参加AMR监测计划，从非常 从2017年开始。FDA Vet-LIRN计划通过以下方式支持网络实验室： 研究和监测用品、设备、人员和 扩大实验室能力和容量所需的其他相关活动。的一部分 在这个项目中，ISU VDL收集细菌分离株，并将它们送到基因组测序中心。 实验室在另一个地方。在本申请中，我们建议继续合作 与Vet-LIRN合作，以实现AMR监测计划的动态目标， 部分内容见FOA PAR-18-604“Vet-LIRN网络能力建设项目”。 具体来说，作为源头实验室，我们将继续分离和鉴定细菌病原体 感兴趣的ISU VDL，进行抗菌药物敏感性测试，并分享分离株， Vet-LIRN网络的抗菌药物敏感性测试结果，每季度一次， Vet-LIRN协议。我们坚信ISU兽医诊断实验室 大大提高了AMR监测项目的能力和有效性， 兽医病原体在过去几年中，并希望继续我们现有的伙伴关系， 与FDA Vet-LIRN合作，以帮助全国/全球打击 抗生素耐药性的研究

Project Summary/Abstract Monitoring antimicrobial resistance among veterinary pathogens at Iowa State University Veterinary Diagnostic Laboratory: A Vet-LIRN AMR Surveillance Project Antimicrobial resistance (AMR) is a major health threat affecting the well-being of humans, animals, and the environment worldwide. In the United States, Veterinary Laboratory Investigation and Response Network (Vet-LIRN) under the Food and Drug Administration, Center for Veterinary Medicine (FDA-CVM) performs surveillance of AMR in select veterinary bacterial pathogens (e.g., Salmonella, Escherichia coli, and Staphylococcus pseudintermedius) from animals by partnering with diagnostic laboratories throughout North America. Under the initiative, Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) became a Vet- LIRN source laboratory and has participated the AMR surveillance program from the very beginning since 2017. The FDA Vet-LIRN program supports the network laboratories via cooperative agreements for research and surveillance for supplies, equipment, personnel and other related activities that are needed to expand laboratories’ capability and capacity. As part of this project, the ISU VDL collects bacterial isolates and sends them to a Genome Sequencing laboratory in another location. In this application, we propose to continue the collaborative partnership with Vet-LIRN to address the dynamic goals of the AMR Surveillance Program as partly described in FOA PAR-18-604 “Vet-LIRN Network Capacity-Building Projects”. Specifically, as a source laboratory, we will continue to isolate and identify bacterial pathogens of interests at ISU VDL, perform antimicrobial susceptibility testing, and share the isolates and antimicrobial susceptibility testing result with Vet-LIRN network on a quarterly basis following the Vet-LIRN protocol. We strongly believe that the ISU Veterinary Diagnostic Laboratory has substantially contributed to the capacity and effectiveness of the AMR surveillance project in veterinary pathogens over the past years, and would like to continue our existing partnership with the FDA Vet-LIRN in order to help the nationwide/global effort on combatting antimicrobial resistance in veterinary settings.