Oscillating Magnetic Field Assisted Supercooling Preservation of Fresh and Unfrozen Ovaries at Subzero Temperatures

振荡磁场辅助在零下温度下过冷保存新鲜和未冷冻的卵巢

• 批准号: 10821622
• 负责人: Soojin Jun
• 金额: $ 27.5万
• 依托单位: JUN INNOVATIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-25 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10821622
• 关键词: 3-Dimensional; Affect; Assisted Reproductive Technology; Automobile Driving; Biocompatible Materials; Biological; Biological Preservation; Butylene Glycols; Cancer Patient; Cardiac Myocytes; Cells; Cellular Structures; Characteristics; Chemicals; Chills; Clinical; Complex; Computer software; Control Groups; Cryopreservation; Cryoprotective Agents; Dehydration; Deterioration; Devices; Dimethyl Sulfoxide; Elements; Engineering; Ensure; Equilibrium; Fertility; Foundations; Freezing; Gene Expression; Goals; Growth; Human; Ice; Injury; Legal patent; Life; Liquid substance; Membrane; Metabolic; Methods; Mus; Neuronal Differentiation; Oocytes; Organ; Organ Preservation; Osmosis; Outcome; Ovarian; Ovarian Tissue; Ovary; Phase; Preservation Technique; Process; Property; Protocols documentation; RNA; Radiation therapy; Recipe; Recovery; Sampling; Solid; Stress; Structure; System; Techniques; Technology; Temperature; Testing; Time; Tissue Preservation; Tissues; Toxic effect; Transplantation; Transportation; Water; biological systems; chemotherapy; cold temperature; commercialization; cytotoxicity; design; extracellular; fertility preservation; granulocyte; hazard; innovation; insight; invention; magnetic field; matrigel; melting; natural hypothermia; novel; organizational structure; phase 1 study; phase change; preservation; tissue culture; transcriptome sequencing

PROJECT SUMMARY/ABSTRACT When the internal temperature of a material during freezing is below its equilibrium freezing point before ice nucleation has occurred, the material is said to be in the supercooled state. The long-term objective of this project is to develop a novel commercially viable supercooling device to preserve biological materials far below their freezing points while retaining their functionalities live. Engineered magnetic fields in the oscillation mode has been proved to control the discharge and realignment of water molecules using diamagnetic properties of water molecules. Therefore, water in biological materials can remain unfrozen at subzero temperatures when magnetic fields are applied. Among many other materials for cryopreservation and organ banking, we have selected the whole mice ovary for our focused proof of concept. The developed supercooling technology will enable an extended storage duration as well as higher recovery rates of ovarian functionalities with zero toxicity, compared to classic cryopreservation methods. In the Phase I study, the supercooling technology will be tested for an extended supercooling status of mice ovaries at -5 to -10 °C for up to 4 weeks, and their preserved fertilities will be examined. We anticipate a robust and stabilized solution for hypothermic yet non-freezing preservation of diverse biological samples, i.e. cells, tissues, and organs, by extension. In the past several years, a number of studies have appeared questioning the functional qualities of biological materials stored using conventional cryopreservation methods. They indicated that (i) biological systems have highly organized structures that are extremely sensitive to freezing/thawing processes and (ii) high concentrations of cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO) are potentially toxic. When the biological sample is cooled below its melting point, or equilibrium freezing temperature, water within the cellular structure will undergo a phase change from liquid to solid. The formation of extracellular ice is known to be a hazard to structured tissues and organs. In addition, DMSO shows high cytotoxicity and affects the differentiation of neuron-like cells, cardiac myocytes, and granulocytes, and needs to be eliminated rapidly after thawing. The developed technique will offer the technical feasibility and solid foundations for any full-organ or complex tissue preservation efforts, providing insight into potential structural and functional effects of the preservation process on high-content, complex and human-derived organs.

项目摘要/摘要 当一种材料在冻结过程中的内部温度低于其平衡冻结时 在冰核发生之前，物质被认为处于过冷状态。这个 该项目的长期目标是开发一种新的商业上可行的过冷装置。 将生物材料保存在远低于冰点的水平，同时保留其 功能仍然存在。工程磁场在振荡模式下已被证明 利用水的抗磁性控制水分子的释放和重新排列 分子。因此，生物材料中的水可以在零下的温度下保持不冻结。 当施加磁场时。在许多其他冷冻保存和器官保存材料中 银行，我们选择了整个小鼠卵巢作为我们的重点概念证明。这个 开发的过冷技术将使储存时间更长，甚至更长 与经典冷冻保存相比，零毒性的卵巢功能恢复率 方法：研究方法。在第一阶段的研究中， 过冷技术将进行更长时间的测试 -5~-10℃下小鼠卵巢的过冷状态 °C保存长达4周，并保存其 将进行受精性检查。我们期待着一个强大而稳定的低温解决方案 通过以下方式非冷冻保存各种生物样本，即细胞、组织和器官 分机。在过去的几年里，出现了一些对功能性 使用常规超低温保存方法储存的生物材料的质量。他们 表明(I)生物系统具有高度组织的结构，这些结构极其敏感 对于冻结/解冻过程和(Ii)高浓度的低温保护剂(CPA)，如 因为二甲基亚砜(DMSO)具有潜在的毒性。当生物样本冷却到下面时 它的熔点，或平衡冰点，细胞结构内的水将 经历从液体到固体的相变。众所周知，细胞外冰的形成是一种 对结构化组织和器官的危害。此外，DMSO表现出很高的细胞毒性和影响 神经元样细胞、心肌细胞和粒细胞的分化，需要 解冻后迅速消除。所开发的技术将提供技术上的可行性和 为任何完整器官或复杂组织的保存工作奠定坚实的基础，提供对 保存过程对高含量、复合体的潜在结构和功能影响 和人类衍生的器官。