Slo2 channels in Olfactory Adaptation

项目摘要 感觉适应是所有感觉系统的一个重要特征，因为它使它们能够保持敏感度 在不同刺激的大范围背景下。在这里，我们介绍了Na+激活的K+(KNA/Slo2) 将一个新的参与者引导到构成感觉适应的机制中。多年来的研究表明 提示在过度兴奋状态下，KNA/Slo2通道可能具有抑制活动的作用。KNA/SLO2 通道的独特之处在于，主要的去极化离子Na+激活了一个重新极化的K+选择性孔道；因此，通过 自然，这些频道似乎被设计成一种内置的超极化机制，以对抗过度的 激动人心。在拟议的研究中，我们使用果蝇作为模型系统，在其中进行基因操作 Dslo2表达，体内嗅觉适应测试，鉴定dslo2所在的细胞和亚细胞结构域 通道影响适应，电生理研究dslo2通道在兴奋性中的作用。 这些细胞。

Project Summary Sensory adaptation is an important feature of all sensory systems, as it allows them to maintain sensitivity over a wide range in backgrounds of varying stimulation. Here, we introduce Na+-activated K+ (KNa/Slo2) channels a new player to the mechanisms that underlie sensory adaptation. Studies over the years have suggested that KNa/Slo2 channels might function to dampen activity in states of excessive excitation. KNa/Slo2 channels are unique in that the major depolarizing ion, Na+, activates a repolarizing K+-selective pore; thus by nature, these channels seem designed to be a built-in hyperpolarizing mechanism against episodes of excessive excitation. In the proposed studies, we use Drosophila as a model system in which to genetically manipulate dSlo2 expression, test for olfactory adaptation in vivo, identify the cells and subcellular domains in which dSlo2 channels affect adaptation, and electrophysiologically examine the role of dSlo2 channels in the excitability of these cells.