Slo2 channels in Olfactory Adaptation

嗅觉适应中的 Slo2 通道

• 批准号: 10823705
• 负责人: SUSAN L TSUNODA
• 金额: $ 41.21万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10823705
• 关键词: Action Potentials; Adult; Affect; Afferent Neurons; Behavioral; Biological Models; Brain; Cations; Cells; Code; Down-Regulation; Drosophila genus; Electrophysiology (science); Genes; Hyperactivity; Image; Ions; Microscopy; Modeling; Nature; Neurons; Olfactory Receptor Neurons; Organ; Orthologous Gene; Output; Play; Potassium Channel; Proteins; Receptor Signaling; Role; Seizures; Sensory; Signal Transduction; Site; Study models; Synapses; System; Testing; biophysical properties; desensitization; design; genetic manipulation; in vivo; insight; knock-down; neuronal excitability; patch clamp; receptor; response; sensory system

Project Summary Sensory adaptation is an important feature of all sensory systems, as it allows them to maintain sensitivity over a wide range in backgrounds of varying stimulation. Here, we introduce Na+-activated K+ (KNa/Slo2) channels a new player to the mechanisms that underlie sensory adaptation. Studies over the years have suggested that KNa/Slo2 channels might function to dampen activity in states of excessive excitation. KNa/Slo2 channels are unique in that the major depolarizing ion, Na+, activates a repolarizing K+-selective pore; thus by nature, these channels seem designed to be a built-in hyperpolarizing mechanism against episodes of excessive excitation. In the proposed studies, we use Drosophila as a model system in which to genetically manipulate dSlo2 expression, test for olfactory adaptation in vivo, identify the cells and subcellular domains in which dSlo2 channels affect adaptation, and electrophysiologically examine the role of dSlo2 channels in the excitability of these cells.

项目摘要 感觉适应是所有感觉系统的一个重要特征，因为它允许它们保持敏感性 在各种刺激的背景下进行。本文介绍了Na+激活的K+（KNa/Slo 2） 引导一个新玩家进入感官适应的机制。多年来的研究表明， KNa/Slo 2通道可能在过度兴奋状态下起抑制活性的作用。KNa/Slo2 通道的独特之处在于，主要的去极化离子Na+激活复极化K+选择性孔;因此， 自然，这些通道似乎被设计成一个内置的超极化机制，以防止过度的事件发生。 激发在拟议的研究中，我们使用果蝇作为一个模型系统，在其中进行遗传操作， dSlo 2表达，体内嗅觉适应测试，鉴定其中dSlo 2表达的细胞和亚细胞结构域。 通道影响适应，并电生理学检查dSlo 2通道的兴奋性的作用， 这些细胞。