Assessing the interplay between stress, health, behavior, and inflammatory gene expression response to wildfire smoke exposures using community engaged and remote sampling approaches

使用社区参与和远程采样方法评估压力、健康、行为和炎症基因表达对野火烟雾暴露的反应之间的相互作用

• 批准号: 10827549
• 负责人: Nicole Ann Errett
• 金额: $ 8.43万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10827549
• 关键词: Affect; Air; American; Area; Back; Behavior; Biological; Biometry; Blood; Blood specimen; Chemistry; Collaborations; Collection; Communication; Communities; Coupled; Data; Development; Distributional Activity; Enrollment; Event; Evidence based intervention; Exposure to; Fire - disasters; Frequencies; Future; Gene Expression; Generations; Genes; Goals; Health; Home; Household; Human; Immune response; Immune system; Individual; Inflammatory; Inflammatory Response; Intervention; Knowledge; Long-Term Effects; Longitudinal Surveys; Longitudinal cohort study; Measurement; Occupational; Pacific Northwest; Participant; Perception; Personal Satisfaction; Persons; Prospective Studies; RNA; Recommendation; Research; Sampling; Science; Seasons; Series; Smoke; Social Impacts; Stress; Surveys; Technology; Time; Toxicology; Translational Research; Whole Blood; Wildfire; air cleaner; behavior change; community engaged research; community engagement; evidence base; experience; exposure reduction interventions; falls; implementation science; improved; indexing; indoor air; insight; member; nano-string; novel; portability; protective effect; response; sample collection; socioeconomics; transcriptome

PROJECT SUMMARY/ABSTRACT As wildfire occurrence and intensity has increased in recent years, understanding the human impact of wildfire smoke (WFS) exposure has become paramount. The impact of WFS on a community can be examined by investigating the proximal deleterious biological effects of WFS exposure, health and well-being and perceived impact of evidence-based exposure reduction interventions at community scale. At the beginning of the 2021 WFS season, we commenced preliminary research to gauge the biological and social impact of WFS in the Methow Valley, WA; a region in the Pacific Northwest with historically high WFS exposure burden, where by early July 2021, had experienced several weeks of WFS exposure that was “nearly off the chart hazardous”[2]. We distributed a novel kit, homeRNA, for self-collection and stabilization of whole blood RNA that allows for the assessment of inflammatory gene response to WFS to 62 participants across the American West, including 18 in the Methow Valley, WA, collecting over 400 stabilized blood samples to date: a subset of which will be analyzed with a NanoString panel of ~800 inflammatory genes. Concomitantly, leveraging the donation and distribution of 2,000 HEPA portable air cleaners (PACs) to community members across Methow Valley, WA, we enrolled and surveyed nearly 1,000 participants to assess their perceived health and well-being during WFS events, and the perceived protective effect of the HEPA PACs. This unprecedented opportunity allows us to simultaneously probe into the inflammatory gene response effects of WFS exposure, and perceptions regarding the health and well-being impacts of WFS exposure and mitigation strategies. Here we seek to assess the biological effect of WFS in the inflammatory gene response by identifying key inflammatory response genes in blood before, during, and after exposure using our homeRNA blood sampling kits. We also aim to assess perceived WFS impacts on health, well-being, and behavior change across socio-economic, demographic, occupational, and household groups, as well as the perceived impacts of HEPA PACs, an evidence-based exposure reduction intervention increasingly used and recommended in WFS-affected communities. Together, these results will inform the development of a subsequent R01 application that will combine homeRNA and survey research to improve mechanistic understanding of the effects of WFS exposure and evidence-based exposure reduction interventions (like HEPA PACs) on inflammatory gene expression, behavior and perceived health and well-being. Our long-standing, genuine partnership with the Methow Valley community honed through years of collaboration enables this research, and uniquely allows for real-time science communication and research translation. As wildfires are expected to increase in frequency and magnitude, enhancing our scientific knowledge about health impacts and their underlying mechanisms, as well the impact of interventions, is of immediate and paramount importance.

项目总结/文摘