MECHANISMS OF VISCERAL PAIN DRIVEN BY SMALL INTESTINAL MICROBIOTA

小肠微生物驱动内脏疼痛的机制

• 批准号: 10836298
• 负责人: Arthur Beyder
• 金额: $ 79.51万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-19 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10836298
• 关键词: 16S ribosomal RNA sequencing; Abdomen; Abdominal Pain; Acetates; Acute; Address; Affect; Afferent Neurons; Animal Model; Bacteria; Cell model; Central Nervous System; Chemicals; Chest; Chronic; Colon; Data; Dedications; Dependovirus; Development; Diagnostic; Diffuse; Disease; Disease remission; Economic Burden; Electrophysiology (science); Enterobacter; Enterobacteriaceae; Enterochromaffin Cells; Epithelial Cells; Exposure to; Feces; Frequencies; Functional disorder; Germ-Free; Growth; Health Care Costs; Human; Hypersensitivity; Image; Individual; Irritable Bowel Syndrome; Klebsiella pneumoniae; Knowledge; Mechanics; Modeling; Molecular; Mus; Nature; Neuroepithelial; Neurons; Neurotransmitters; Organoids; Outcome; Pain; Pathology; Pathway interactions; Patients; Physiological; Population; Preparation; Probiotics; Quality of life; Relapse; Role; Sampling; Sensory; Serotonin; Signal Transduction; Site; Small Intestines; Spinal Cord; Spinal Ganglia; Stimulus; Structure; Symptoms; Testing; Therapeutic; Transducers; Transgenic Mice; Tyrosine; Visceral; Visceral pain; colon bacteria; design; effective therapy; ganglion cell; gastrointestinal symptom; genome sequencing; gut microbiome; gut microbiota; mechanical stimulus; microbial; microbial composition; microbial products; microbiome; nerve supply; neural; neurotransmitter release; new therapeutic target; novel; novel strategies; optogenetics; productivity loss; response; sensory input; testing access; tool; transmission process; whole genome

PROJECT SUMMARY/ABSTRACT Irritable bowel syndrome is a globally prevalent disorder (~11%) characterized by an alteration in stool form/frequency and abdominal pain one or more days per week. Abdominal pain in IBS, like other forms of visceral pain, is often diffuse and poorly localized, making it difficult to delineate the site of pathology, and as a result there are few therapeutic options. Gut microbial products have been shown to be important luminal signals for abdominal pain, but these studies have largely focused on the colon. We recently found that small intestinal microbial composition is associated with gastrointestinal (GI) symptoms like abdominal pain, but the mechanisms underlying the role of small intestinal microbiota/microbial products in the pathophysiology of abdominal pain remains a critical knowledge gap. To address this gap, we will elucidate the sensory innervation of the proximal small intestine and identify the cellular and molecular pathways by which the small intestine detects and transduces luminal microbial signals that contribute to visceral hypersensitivity. In our extensive preliminary studies, we established a dedicated model to study the physiologic effects of human small intestinal microbiome in germ free (GF) mice, metabolite effects on isolated DRGs and epithelial cells, and a novel ex vivo spinal cord- small intestine preparation to study the transmission of luminal signals to the spinal cord via luminal metabolite signaling through (1) EC cells, that are epithelial cells which signal to neurons via serotonin, a neurotransmitter in the gut that modulates visceral pain, and (2) sensory neurons in dorsal root ganglia (DRG), the first-order afferent neurons of pain pathways. Using these models, we identified distinct bacteria and bacterial metabolites that activate EC cells and thoracic DRG neurons. Based on our preliminary findings, we hypothesize that small intestinal bacterial products contribute to visceral hypersensitivity by activating small intestine sensory afferents directly and through neuro-epithelial connections by activating EC cells. We will address the hypothesis in two Specific Aims using cutting-edge stimulation/acquisition approaches, including combination of Ca2+ imaging in organoids, electrophysiology of EC cells and DRG neurons, ex vivo preparations from novel transgenic mice, and adeno-associated viruses (AAVs) characterizing the sensory input from the small intestine, and optogenetics to study neuro-epithelial signaling. In Specific Aim 1, we will determine mechanisms underlying activation of EC cells and DRG neurons, and in Specific Aim 2, we will determine the sensory transduction pathways involved in responding to distinct microbial products. These studies will be the first to provide a functional and molecular characterization of sensory neurons in the DRG that innervate the small intestine, determine which subpopulations are activated and/or sensitized by microbial products in the lumen, and test whether EC cells are involved in the sensory transduction pathway. Our findings will allow the development of novel microbial therapies for abdominal pain that target distinct microbial pathways in the small intestine.

项目总结/文摘