Supplement for Johns Hopkins Human Temporal Bone Resource

约翰霍普金斯大学人类颞骨资源补充剂

• 批准号: 10837479
• 负责人: John Patrick Carey
• 金额: $ 12.09万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10837479
• 关键词: Acceleration; Award; Biological; Cadaver; Collection; Communication; Communities; Data; Disease; Ear; Educational process of instructing; Electron Microscopy; Equilibrium; Harvest; Hearing; Histologic; Histology; Human; Image; Infrastructure; Knowledge; Measurement; Mentors; Methods; Microscopy; Morphology; Organ; Parents; Postdoctoral Fellow; Process; Protocols documentation; Research; Research Personnel; Resolution; Resources; Scientist; Specimen; Stains; Structure; Temporal bone structure; Testing; Thick; Visualization; Work; analysis pipeline; bilingualism; data acquisition; outreach; parent grant; side effect; three dimensional structure; tissue processing; tool; web site

Research Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the process is difficult, expensive, and requires expertise that only a small number of active investigators have. In the parent award, we will make the Johns Hopkins human temporal bone collection more accessible to the scientific community, re-establish the infrastructure for harvesting, imaging, and processing new specimens, and develop teaching and outreach activities to increase engagement in human temporal bone research. We partner with scientists specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop an inclusive, diverse scientific resource. Here we propose a supplement to the Parent grant (Johns Hopkins Human Temporal Bone Resource) to support a postdoctoral fellow who will work with our team to investigate methods for visualization and quantitative analysis of the same temporal bones as intact structures, as thick sections for gross histological assessment, and as semi- and ultrathin sections suitable for fine structure analysis using electron microscopy. For this supplement, we will 1) optimize the temporal bone processing pipeline to enhance quality and resolution of morphological data acquisition and 2) establish a workflow to analyze and correlate structural data derived from whole-organ imaging down to ultrastructural microscopy. The tissue processing protocols and workflows to be developed will enhance Aim 2 of the parent grant and will be useful for otopathology studies and assessing the feasibility/histological side effects of potential hearing and balance treatments in human cadaver ears. Additionally, the analysis protocols generated as part of this supplement will be useful in furthering our understanding of how the 3D structure of the ear relates to functional measurements derived from audiometric and vestibular tests. All protocols and analysis routines will be shared with the scientific community via the website to be developed as part of Aim 3 of the parent grant. Sharing these tools will accelerate advances in and knowledge of otopathology.

颞骨的研究对于我们更好地理解 耳科疾病;然而，该过程是困难的，昂贵的，需要专业知识，只有少数 积极的调查人员。在家长奖中，我们将把约翰霍普金斯人类颞骨收藏 更容易接近科学界，重新建立基础设施的收获，成像， 处理新标本，并开展教学和外联活动，以增加人类参与 颞骨研究我们与专门从事生物可视化、双语科学 交流，并指导不同的科学家开发一个包容性的，多样化的科学资源。这里我们 建议补充父母补助金（约翰霍普金斯人类颞骨资源），以支持 博士后研究员，他将与我们的团队一起研究可视化和定量分析的方法 作为完整结构的相同颞骨，作为粗组织学评估的厚切片，以及作为 半切片和半切片，适合于使用电子显微镜进行精细结构分析。对于这种补充， 我们将1）优化颞骨处理流水线，以提高形态学的质量和分辨率， 数据采集和2）建立工作流程以分析和关联来自整个器官的结构数据 成像到超微结构显微镜。待开发的组织处理方案和工作流程将 加强目标2的父母补助金，将有助于耳病理学研究和评估 在人尸体耳中进行潜在听力和平衡治疗的可行性/组织学副作用。 此外，作为本补充的一部分生成的分析方案将有助于进一步促进我们的 了解耳朵的3D结构如何与来自听力测定的功能测量相关 和前庭测试所有协议和分析程序将通过 网站将作为母公司补助金目标3的一部分开发。共享这些工具将加速 耳科病理学知识。