Supplement for Johns Hopkins Human Temporal Bone Resource

研究综述：对颞骨的研究对于提高我们对骨质疏松症发病机制的认识是必不可少的。 耳科疾病；然而，这一过程困难、昂贵，并且只需要一小部分 积极的调查人员已经。在家长奖中，我们将把约翰霍普金斯大学的人类颞骨收藏 科学界更容易访问，重新建立用于采集、成像和 处理新标本，并开展教学和推广活动，以增加对人类的参与 关于颞骨的研究。我们与擅长生物可视化、双语科学的科学家合作 交流，并指导不同的科学家开发包容性的、多样化的科学资源。在这里我们 建议对父母赠款(约翰·霍普金斯人类颞骨资源)进行补充，以支持 博士后，将与我们的团队合作，研究可视化和定量分析的方法 作为完整结构的相同的颞骨，作为大体组织学评估的厚切片，以及作为 适合用电子显微镜进行精细结构分析的半薄和超薄切片。在这份增刊中， 我们将1)优化颞骨处理流水线，以提高形态的质量和分辨率 数据获取和2)建立分析和关联来自整个器官的结构数据的工作流程 成像一直延伸到超微结构显微镜。将制定的组织处理方案和工作流程将 加强家长资助的目标2，有助耳科病理学研究和评估 人类身体耳潜在听力和平衡治疗的可行性/组织学副作用。 此外，作为本补充材料的一部分生成的分析方案将有助于进一步 理解耳朵的3D结构与听力测量得出的功能测量之间的关系 还有前庭测试。所有的方案和分析程序都将通过 网站将作为父母资助的目标3的一部分开发。共享这些工具将加快在和 耳科病理学知识。

Research Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the process is difficult, expensive, and requires expertise that only a small number of active investigators have. In the parent award, we will make the Johns Hopkins human temporal bone collection more accessible to the scientific community, re-establish the infrastructure for harvesting, imaging, and processing new specimens, and develop teaching and outreach activities to increase engagement in human temporal bone research. We partner with scientists specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop an inclusive, diverse scientific resource. Here we propose a supplement to the Parent grant (Johns Hopkins Human Temporal Bone Resource) to support a postdoctoral fellow who will work with our team to investigate methods for visualization and quantitative analysis of the same temporal bones as intact structures, as thick sections for gross histological assessment, and as semi- and ultrathin sections suitable for fine structure analysis using electron microscopy. For this supplement, we will 1) optimize the temporal bone processing pipeline to enhance quality and resolution of morphological data acquisition and 2) establish a workflow to analyze and correlate structural data derived from whole-organ imaging down to ultrastructural microscopy. The tissue processing protocols and workflows to be developed will enhance Aim 2 of the parent grant and will be useful for otopathology studies and assessing the feasibility/histological side effects of potential hearing and balance treatments in human cadaver ears. Additionally, the analysis protocols generated as part of this supplement will be useful in furthering our understanding of how the 3D structure of the ear relates to functional measurements derived from audiometric and vestibular tests. All protocols and analysis routines will be shared with the scientific community via the website to be developed as part of Aim 3 of the parent grant. Sharing these tools will accelerate advances in and knowledge of otopathology.