Johns Hopkins Human Temporal Bone Resource

项目摘要 颞骨的研究是必不可少的，以完善我们的理解机制的耳科疾病; 然而，进入该组织的困难、通常较长的处理时间和费用已经 导致从事这项工作的实验室数量不断减少。在20世纪20年代，第一个人类颞骨 美国的第一个实验室是在约翰霍普金斯大学建立的。该集合包含约1400个处理过的 来自患有一系列耳科疾病的捐赠者的标本，主要是在20世纪20年代获得的， 20世纪70年代，但在过去的20年里，只有少数研究利用了这一收集。我们建议 科学界更容易获得收集的资料，并重建收集的基础设施， 成像和处理新标本，目的是从基于人群的 参与者已经同意进行尸检和大脑捐赠的衰老研究 在约翰霍普金斯，以及其他临床耳科研究正在进行的巴尔的摩大都会地区。我们将 与专业从事生物可视化、双语科学交流和指导的教师合作 不同的科学家开发包容性的外联工具。我们还将与巴尔的摩的转录组学公司合作， 资源，以建立在非人灵长类动物和人类中进行空间转录组学分析的方案 颞骨我们将通过三个具体目标来实现这些目标：1）目录，优先级，以及 来自约翰霍普金斯人类颞骨收集的现有标本; 2）开发有效的采集， 新标本的快速组织学和空间转录组学工作流程，并建立收集 对流行病学研究参与者进行尸检时收集的样本; 3）教育/宣传 鼓励其他研究者参与颞骨研究的活动。 约翰霍普金斯大学的马克斯·布洛德尔的大量颞骨收藏和内耳插图也做出了贡献 这对我们早期了解耳朵的许多疾病有很大的帮助，比如发现了球体， 老年性耳聋的组织病理学相关性和囊状斑的神经支配。该系列还 显示出持久的价值，有助于我们了解开裂的上级半圆的患病率， 以及黑色素在耳蜗和前庭保护中的作用。共享 这一宝贵的收集与其他研究人员，同时应用新技术在数字处理， 教育将加速耳病理学的进步和知识。我们期待着重振这一 通过开发新的收藏，应用现代技术和整合耳病理学， 以人群为基础的研究，包括全面的耳科测试和功能措施。

Project Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the difficulty in accessing this tissue, the typically long processing times, and the expense have contributed to a dwindling number of labs engaged in this work. In the 1920s, the first human temporal bone laboratory in the U.S. was established at Johns Hopkins. The collection contains approximately ~1400 processed specimens from donors with a range of otologic diseases that were obtained primarily in the 1920s through 1970s, but only a handful of studies have made use of this collection in the past 20 years. We propose to make the collection more accessible to the scientific community and to re-establish the infrastructure for harvesting, imaging, and processing new specimens with the intention of recruiting new donors from population-based studies of aging in which participants have already consented to autopsies and brain donations being performed at Johns Hopkins, as well as other clinical otological studies ongoing in the Baltimore Metro Region. We will partner with faculty specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop inclusive outreach tools. We will also partner with Baltimore-based transcriptomics resources to establish protocols for performing spatial transcriptomics analysis in nonhuman primate and human temporal bones. We will approach these objectives with three Specific Aims: 1) Catalog, prioritize, and digitize existing specimens from the Johns Hopkins Human Temporal Bone Collection; 2) Develop efficient harvesting, rapid histology, and spatial transcriptomics workflows for new specimens and establish partnerships for collecting specimens during autopsies performed on participants in epidemiological studies; 3) Education/outreach activities to encourage other investigators to engage in temporal bone research. The large temporal bone collection and the inner ear illustrations of Max Brödel at Johns Hopkins contributed greatly to our early understanding of many diseases of the ear, such as the discovery of glomus bodies, the histopathologic correlates of presbycusis, and innervation of the saccular maculae. The collection has also shown enduring value, contributing to our understanding of the prevalence of a dehiscent superior semicircular canal and arachnoid granulations as well as the role of melanin in cochlear and vestibular protection. Sharing this valuable collection with other researchers while applying novel techniques in digital processing and education will accelerate advances in and knowledge of otopathology. We look forward to reinvigorating this tradition of excellence by developing a new collection, applying modern techniques, and integrating otopathology with population-based studies that include comprehensive otologic testing and functional measures.