Johns Hopkins Human Temporal Bone Resource

项目摘要 对颞骨的研究对于完善我们对耳科疾病发病机制的理解是必不可少的； 然而，获取该组织的困难、通常较长的处理时间以及费用 导致从事这项工作的实验室数量不断减少。在20世纪20年代，人类第一块颞骨 美国的实验室是在约翰霍普金斯大学建立的。该集合包含大约1400个已处理的 来自患有一系列耳科疾病的捐赠者的样本，主要是在20世纪20年代至 20世纪70年代，但在过去的20年里，只有少数几项研究利用了这一收藏。我们打算做一件 科学界更容易获得这些收集品，并重建收割基础设施， 对新样本进行成像和处理，意在从人群中招募新的捐赠者 参与者已经同意进行尸检和脑捐赠的衰老研究 在约翰霍普金斯大学，以及巴尔的摩大都会地区正在进行的其他临床耳科研究。我们会 与专攻生物可视化、双语科学交流和辅导的教师合作 不同的科学家共同开发包容各方的外联工具。我们还将与总部位于巴尔的摩的转录基因组公司合作 建立在非人灵长类和人类中进行空间转录分析的方案的资源 颞骨。我们将通过三个具体目标来实现这些目标：1)目录、优先顺序和数字化 来自约翰·霍普金斯人类颞骨收藏的现有标本；2)开发有效的采集， 新标本的快速组织学和空间转录学工作流程，并建立收集合作伙伴关系 对流行病学研究参与者进行尸检时的标本；3)教育/外展 鼓励其他研究人员从事颞骨研究的活动。 约翰霍普金斯大学的大量颞骨收藏和Max Brödel的内耳插图对此有贡献 很大程度上有助于我们对许多耳部疾病的早期了解，例如球体的发现， 老年性耳聋和球囊黄斑神经支配的组织病理学相关性。收藏品还包括 显示出持久的价值，有助于我们了解上半圆形裂开的流行程度 管状和蛛网膜颗粒，以及黑色素在耳蜗和前庭保护中的作用。共享 这是与其他研究人员在数字处理和应用新技术的同时收集的宝贵资料 教育将加速耳科病理学的进步和知识。我们期待着重振这一局面。 通过开发新的集合、应用现代技术和整合耳科病理学来实现卓越的传统 以人群为基础的研究，包括全面的耳科测试和功能测量。

Project Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the difficulty in accessing this tissue, the typically long processing times, and the expense have contributed to a dwindling number of labs engaged in this work. In the 1920s, the first human temporal bone laboratory in the U.S. was established at Johns Hopkins. The collection contains approximately ~1400 processed specimens from donors with a range of otologic diseases that were obtained primarily in the 1920s through 1970s, but only a handful of studies have made use of this collection in the past 20 years. We propose to make the collection more accessible to the scientific community and to re-establish the infrastructure for harvesting, imaging, and processing new specimens with the intention of recruiting new donors from population-based studies of aging in which participants have already consented to autopsies and brain donations being performed at Johns Hopkins, as well as other clinical otological studies ongoing in the Baltimore Metro Region. We will partner with faculty specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop inclusive outreach tools. We will also partner with Baltimore-based transcriptomics resources to establish protocols for performing spatial transcriptomics analysis in nonhuman primate and human temporal bones. We will approach these objectives with three Specific Aims: 1) Catalog, prioritize, and digitize existing specimens from the Johns Hopkins Human Temporal Bone Collection; 2) Develop efficient harvesting, rapid histology, and spatial transcriptomics workflows for new specimens and establish partnerships for collecting specimens during autopsies performed on participants in epidemiological studies; 3) Education/outreach activities to encourage other investigators to engage in temporal bone research. The large temporal bone collection and the inner ear illustrations of Max Brödel at Johns Hopkins contributed greatly to our early understanding of many diseases of the ear, such as the discovery of glomus bodies, the histopathologic correlates of presbycusis, and innervation of the saccular maculae. The collection has also shown enduring value, contributing to our understanding of the prevalence of a dehiscent superior semicircular canal and arachnoid granulations as well as the role of melanin in cochlear and vestibular protection. Sharing this valuable collection with other researchers while applying novel techniques in digital processing and education will accelerate advances in and knowledge of otopathology. We look forward to reinvigorating this tradition of excellence by developing a new collection, applying modern techniques, and integrating otopathology with population-based studies that include comprehensive otologic testing and functional measures.