Investigating obesity-induced altered ovarian intracellular signaling

翻译后摘要：这项研究提出的是对气候变化的健康影响，特别是与女性 生殖和一般健康，在瘦和肥胖的女性。由于肥胖影响了约40%的女性， 不成比例地影响少数群体女孩和妇女，这项建议与《公约》的健康公平方面有关。 气候变化和健康倡议。这项工作解决了已确定的相关领域， 气候变化引起的压力对生殖和发育的影响， （DMBA）和非化学应激源（肥胖和热应激）。气候变化和 伴随的严重HS事件的频率是有增无减的，因此，了解HS对卵巢癌的影响， 功能是迫切需要的。Keating博士在这项研究中处于独特的地位，因为她在以下方面的科学记录： 卵巢生物学的这一领域（20篇关于热应激生物学的出版物，其中大部分与 复制）和进行这些研究的技术可行性。假设热应激会导致 高胰岛素血症，改变卵巢类固醇生成，DNA修复，氧化应激和化学 在DMBA暴露期间，生物转化蛋白丰富，加速卵巢毒性，这将 在肥胖小鼠中比瘦小鼠更明显。这一假设是基于我们的强有力的初步数据， 雌性猪生物医学模型和肥胖期间观察到的代谢变化的相关性。这 补充将产生数据，为未来探索热应力对 使用易处理的小鼠模型研究卵毒性的具体途径。

Abstract: The research proposed is on Health Effects of climate change, specifically related to female reproductive and general health, in lean and obese females. Since obesity affects ~40% of women, and disproportionately impacts minority girls and women, this proposal is relevant to the Health Equity aspects of the climate change and health initiative. This work addresses \ the identified relevant areas of understanding the effects of climate-change-induced stress on reproduction and development and assesses multiple chemical (DMBA) and non-chemical stressors (obesity and heat stress) across the lifespan. Climate change and accompanying frequency of severe HS events is unabating, thus, understanding impacts of HS on ovarian function is desperately needed. Dr. Keating is uniquely positioned for this study due to her scientific record in this area of ovarian biology (twenty publications on heat stress biology with the majority related to reproduction) and the technical feasibility to conduct these studies. The hypothesis is that heat stress will cause hyperinsulinemia, altering ovarian steroidogenic, DNA repair, oxidative stress and chemical biotransformation protein abundance, accelerating ovarian toxicity during DMBA exposure, and this will be heightened in obese relative to lean mice. This hypothesis is based upon our strong preliminary data in female pig biomedical models and the relevance to the metabolic changes observed during obesity. This supplement will generate data to pave the way for future exploration into the additive impact of heat stress on ovotoxicity using tractable mouse models to delve into specific pathways.