Early life metal exposure, the gut microbiome, and neurodevelopment in childhood

生命早期的金属暴露、肠道微生物组和儿童期的神经发育

• 批准号: 10883857
• 负责人: Shoshannah Iylene Eggers
• 金额: $ 24.89万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-26 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10883857
• 关键词: 11 year old; Address; Adolescence; Age; Area; Attention; Behavioral; Biological; Biological Markers; Biology; Biometry; Birth; Blood; Brain; Chemical Exposure; Chemicals; Childhood; Cities; Clinical; Collaborations; Copper; Cross-Sectional Studies; Data; Data Analyses; Development; Environment; Environmental Epidemiology; Environmental Exposure; Environmental Health; Epidemiologist; Faculty; Faith; Feces; Foundations; Funding; Future; Goals; Growth; Hair; Health; Human; Human Microbiome; Hydrocortisone; Individual; Infant; Intervention; Knowledge; Laboratories; Lead; Learning; Life; Link; Manganese; Measurement; Measures; Mediation; Mentors; Mentorship; Metabolic; Metagenomics; Metal exposure; Metals; Methodology; Mexico; Microbiology; Modeling; National Institute of Environmental Health Sciences; Neurologic; Neurological outcome; Obesity; Observational Study; Pathway interactions; Play; Positioning Attribute; Predisposition; Proteobacteria; Protocols documentation; Research; Research Activity; Research Personnel; Research Proposals; Resolution; Role; Rotation; Second Pregnancy Trimester; Secure; Shapes; Shotguns; Time; Tooth structure; Training; Training Activity; Work; Zinc; behavior measurement; career; career development; cohort; early detection biomarkers; early life exposure; epidemiology study; executive function; fetal; gut bacteria; gut microbiome; gut-brain axis; infancy; innovation; interest; meetings; metabolome; metabolomics; metagenomic sequencing; microbial; microbiome; microbiome analysis; microbiome composition; microbiome research; multidimensional data; multidisciplinary; multiple omics; neurobehavioral; neurodevelopment; neurotoxicology; novel; pilot test; prenatal; prenatal exposure; prospective; research and development; sample collection; skill acquisition; skills; social stressor; stool sample; success; symposium; tenure track; time use

PROJECT SUMMARY I am an environmental epidemiologist and microbiome researcher with a primary research interest in the role of the human microbiome between environmental exposures and human health, including neurological outcomes. The goal of this proposal is to obtain training and acquire the skills needed to continue to build my academic research career by linking early life metal exposure, the gut microbiome and metabolome in childhood, and neurodevelopmental measures of attention. For this proposal, I plan to train with an expert team of mentors, collaborators, and advisors with transdisciplinary expertise in exposomics, exposure biology, neurodevelopment, biostatistics, metabolomics, epidemiology, and microbiome research. The proposed training plan includes clinical and laboratory rotations, coursework, seminars and meetings, conferences, and 1-on-1 tutorials with mentors and advisors, covering research and career development topics. This training will provide skills and knowledge in the areas of neurodevelopment, and exposomics, including exposure biology and multi-omics analysis. Upon completion of this training plan, I will be able to achieve my long-term career goal of becoming an independent researcher focused on the inter-relationships among environmental exposures, the microbiome, and neurodevelopment. To that end, I propose leveraging the use of the established Programming Research in Obesity, GRowth, Environment and Social Stressors (PROGRESS) birth cohort, based in Mexico City, and adding microbiome analysis. Specifically, I will: 1) train with Dr. Arora in exposure biology and analysis of baby teeth as a biomarker of early life metal exposure; 2) train with Dr. Wright, in collaboration with Dr. Shamesh, in neurodevelopmental assessment, principals of neurotoxicology, and their use in epidemiologic studies; 3) train with Dr. Faith in metagenomic analysis, 4) integrate biostatistical assessment of mixtures and high dimensional data, guided by Dr. Gennings; 4) learn about stool metabolomic analysis methodology with Dr. Walker; 5) work with Dr. Tellez-Rojo to facilitate stool sample collection and data analysis in PROGRESS. I will use these training and research activities to integrate the microbiome into an exposomic analysis of neurodevelopment. I will use the research and career development training I receive to establish myself as an independent investigator with a tenure track faculty position, able to secure R01 funding. This research proposal combines innovative measures of exposure, novel data analysis approaches, and high resolution omics analysis to address gaps in the understanding of timing and potential mechanisms by which metal exposures, and the gut microbiome and metabolome, are associated with neurodevelopmental measures of attention. The proposed research and training plan builds the foundation for an independent research career that aims to clarify the links between environmental exposures and neurodevelopment, via the human microbiome.

项目摘要 我是一名环境流行病学家和微生物组研究员，主要研究兴趣是 环境暴露与人类健康之间的人类微生物组，包括神经学结果。 这个建议的目标是获得培训和获得所需的技能，继续建立我的学术 通过将早期生活中的金属暴露，儿童时期的肠道微生物组和代谢组联系起来， 注意力的神经发育指标 对于这个提议，我计划与一个由导师、合作者和顾问组成的专家团队一起进行培训， 跨学科的专业知识，在生物学，暴露生物学，神经发育，生物统计学，代谢组学， 流行病学和微生物组研究。拟议的培训计划包括临床和实验室轮换， 课程，研讨会和会议，会议，以及与导师和顾问的1对1教程，包括 研究和职业发展主题。这项培训将提供以下领域的技能和知识： 神经发育和神经组学，包括暴露生物学和多组学分析。完成后 通过这个培训计划，我将能够实现我成为一名独立研究人员的长期职业目标 重点关注环境暴露、微生物组和神经发育之间的相互关系。 为此，我建议利用现有的肥胖规划研究，增长， 环境和社会压力源（PROGRESS）出生队列，位于墨西哥城，并添加微生物组 分析.具体来说，我将：1）与阿罗拉博士一起培训暴露生物学和婴儿牙齿分析， 早期生命金属暴露生物标志物; 2）与Shamesh博士合作，与Wright博士一起培训， 神经发育评估，神经毒理学原理及其在流行病学研究中的应用; 3）培训 与Faith博士一起进行宏基因组分析，4）整合混合物和高维的生物统计评估 数据，由Gennings博士指导; 4）与步行者博士一起学习粪便代谢组学分析方法; 5）工作 与Tellez-Rojo博士合作，以促进PROGRESS中的粪便样本收集和数据分析。我会用这些 培训和研究活动，以将微生物组纳入神经发育的基因组学分析。我 我将利用我所接受的研究和职业发展培训来建立自己作为一个独立的 拥有终身教职职位的调查员，能够获得R 01资金。 这项研究提案结合了创新的暴露措施，新颖的数据分析方法， 和高分辨率组学分析，以解决对时间和潜在机制的理解方面的差距 金属暴露、肠道微生物组和代谢组与神经发育相关 注意力的措施。拟议的研究和培训计划为建立一个独立的 研究生涯，旨在澄清环境暴露和神经发育之间的联系，通过 人类微生物组。